FastQCFastQC Report
Fri 10 Feb 2017
SRR2568646_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568646_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719510
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27000.038.0004271
GTACATG82350.034.4357261
TACATGG81400.033.8455922
GGTATCA17050.033.431461
TCAACGC29800.032.357154
CAACGCA30050.031.9298885
ATCAACG30350.031.926113
ACATGGG81900.031.839263
AACGCAG32200.029.9454366
TATCAAC35800.027.3327082
CATGGGG59300.026.9939064
ACGCAGA40400.023.6322567
GTATAGC11400.023.3335971
ATGGGGG34900.022.7290635
ACCGTAT2955.456968E-1222.5422128
CGCAGAG42650.022.0514268
GTATAAT12350.021.1540831
GAGTACA50750.020.9657521
GTACCGT4650.020.4299626
AGTACAT49050.019.7555732
Produced by FastQC (version 0.11.5)