FastQCFastQC Report
Fri 10 Feb 2017
SRR2568646_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568646_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719510
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16100.036.340411
GTATCAA25700.034.796481
GTACATG75000.032.345921
TACATGG74650.031.601412
TCAACGC28300.030.053684
ACATGGG76700.029.9214613
CAACGCA29600.028.573235
ATCAACG29650.028.5250473
AACGCAG30600.027.7947436
TATCAAC34300.024.9593352
CATGGGG53900.024.771294
GAGTACA50000.023.2129551
ACGCAGA37700.022.181277
AGTACAT49450.021.6406762
GTATAGG10650.021.4388851
CGCAGAG41050.020.3643638
GTATAGC10550.019.838591
GTATACG1707.872545E-519.586631
TACCGTA2502.1683627E-719.0053167
ACCGTAT3506.366463E-1118.9990258
Produced by FastQC (version 0.11.5)