FastQCFastQC Report
Fri 10 Feb 2017
SRR2568645_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568645_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1262064
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC83020.6578113312795547No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC54370.43080224140772577No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG44280.35085383942494197No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG40160.3182089022426755No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39750.31496025558133345No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT37700.29871702227462316No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT36070.2858016709136779No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC32330.2561676745394845No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT30460.24135067635238783No Hit
TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC29040.2300992659643251No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC28880.22883150141355746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28110.22273038451298827No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT27660.2191647967139543No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG26240.20791338632589157No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT26160.2072795040505077No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC25830.2046647396645495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22820.18081491905323344No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT21020.16655256785709757No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA20790.1647301563153691No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA20670.16377933290229338No Hit
CTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT20500.16243233306710278No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG20440.16195692136056491No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT19930.1579159218549931No Hit
CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG19480.15435033405595913No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC19230.1523694519453847No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG18800.14896233471519668No Hit
CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA18660.14785304073327502No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA18200.1442082176498181No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC18170.14397051179654916No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA17760.14072186513520707No Hit
CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG17530.13889945359347863No Hit
CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC17230.1365223950607893No Hit
GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA17210.13636392449194334No Hit
ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA17060.1351753952255987No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC16780.13295680726175535No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA16660.13200598384867962No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC16580.1313721015732958No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT16340.12947045474714436No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA16240.1286781019029146No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA16100.1275688079209929No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT15820.12535021995714954No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA15540.1231316319933062No Hit
CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT15510.12289392614003727No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT15260.12091304402946286No Hit
GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG15240.12075457346061688No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA15130.11988298533196415No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA15030.11909063248773438No Hit
ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT14940.11837751492792757No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC14940.11837751492792757No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG14900.11806057379023568No Hit
CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA14900.11806057379023568No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC14780.11710975037715995No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC14780.11710975037715995No Hit
TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC14390.11401957428466385No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT14310.11338569200928003No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG14310.11338569200928003No Hit
ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC14230.11275180973389623No Hit
ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT14180.11235563331178135No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT14080.11156328046755158No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT14060.1114048098987056No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT14040.11124633932985965No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG13830.10958239835697715No Hit
GATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGC13780.10918622193486224No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT13690.10847310437505546No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA13490.10688839868659593No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA13330.10562063413582828No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC13300.10538292828255938No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT13210.10466981072275257No Hit
ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA13200.1045905754383296No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC13150.10419439901621472No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC12920.10237198747448624No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA12790.10134192877698754No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT12790.10134192877698754No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC12680.1004703406483348No Hit
AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA12650.10023263479506585No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG250.00501959257.0051572
CCTTTAG2600.056.6397441
CTTTAGG2850.051.671342
CATTCCG3000.049.081949
GGCATTC3050.048.2773177
CCGGGTC502.8276289E-547.498659
ACCTGGG1900.044.9987263
GGGATGC1800.042.2210277
TAGGCAT3650.040.341325
GTTATAT608.286796E-539.5869141
ATATGAC500.00161515838.0034372
GGTATAG756.9185226E-638.0034371
CATGGAT1003.0318915E-837.998924
TGGGTAT4750.037.998926
ATGTATT1401.0913936E-1137.3248061
GGTATCA27700.037.0430641
TACCTGG2450.034.901122
CATGGGT8050.034.812684
GTATCAA46100.034.6235241
GACATAT4850.034.2814521
Produced by FastQC (version 0.11.5)