##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568645_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1262064 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04387891580776 34.0 31.0 34.0 30.0 34.0 2 32.19615328541183 34.0 31.0 34.0 30.0 34.0 3 32.19213843355012 34.0 31.0 34.0 30.0 34.0 4 35.57701986587051 37.0 35.0 37.0 35.0 37.0 5 35.634154052409386 37.0 35.0 37.0 35.0 37.0 6 35.76325368602543 37.0 37.0 37.0 35.0 37.0 7 35.73760997857478 37.0 37.0 37.0 35.0 37.0 8 35.75235724971159 37.0 37.0 37.0 35.0 37.0 9 37.533281196516185 39.0 38.0 39.0 35.0 39.0 10-11 37.49332006934672 39.0 38.0 39.0 35.0 39.0 12-13 37.50479413088401 39.0 38.0 39.0 35.0 39.0 14-15 38.88755047287618 41.0 39.0 41.0 36.0 41.0 16-17 38.86710895802432 41.0 39.0 41.0 36.0 41.0 18-19 38.924940811242536 41.0 39.0 41.0 36.0 41.0 20-21 38.95802867366473 41.0 39.0 41.0 36.0 41.0 22-23 38.849363423724945 41.0 39.0 41.0 35.0 41.0 24-25 38.792957013273494 41.0 39.0 41.0 35.0 41.0 26-27 38.70733813816098 41.0 39.0 41.0 35.0 41.0 28-29 38.59239468045995 41.0 39.0 41.0 34.0 41.0 30-31 38.46624299560086 40.0 38.0 41.0 34.0 41.0 32-33 38.343604207076666 40.0 38.0 41.0 34.0 41.0 34-35 38.23945140658477 40.0 38.0 41.0 34.0 41.0 36-37 38.11518948325917 40.0 38.0 41.0 33.5 41.0 38-39 38.00616490130453 40.0 38.0 41.0 33.0 41.0 40-41 37.91848630497344 40.0 38.0 41.0 33.0 41.0 42-43 37.752350514712404 40.0 38.0 41.0 33.0 41.0 44-45 37.563560564282 40.0 38.0 41.0 32.0 41.0 46-47 37.55711001977713 40.0 38.0 41.0 32.5 41.0 48-49 37.66888921639473 40.0 38.0 41.0 33.0 41.0 50-51 36.60914462341054 39.0 36.5 40.0 31.0 40.5 52-53 36.763805163605014 39.5 37.5 40.0 31.0 40.5 54-55 37.388508823641274 40.0 38.0 41.0 31.5 41.0 56-57 37.39796119689652 40.0 38.0 41.0 31.5 41.0 58-59 37.295935071438535 40.0 38.0 41.0 31.0 41.0 60-61 37.129633679433056 40.0 37.0 41.0 31.0 41.0 62-63 36.90163454468236 40.0 37.0 41.0 30.5 41.0 64-65 36.78426886433651 39.0 36.5 41.0 30.5 41.0 66-67 36.673977706360375 39.0 36.0 41.0 30.5 41.0 68-69 36.36367608932669 39.0 36.0 41.0 30.0 41.0 70-71 35.9998692617807 38.5 35.0 40.5 29.5 41.0 72-73 35.63720817644747 37.5 35.0 40.0 29.0 41.0 74-75 35.225676748564254 37.0 35.0 39.0 29.0 41.0 76-77 34.83844083976724 36.5 35.0 39.0 29.0 41.0 78-79 34.44365341218829 36.0 35.0 38.5 28.5 40.0 80-81 34.02198739525095 36.0 34.0 37.0 28.0 39.0 82-83 33.63888004094879 35.0 34.0 37.0 27.5 39.0 84-85 33.23326392322418 35.0 34.0 36.5 26.5 38.0 86-87 32.99200317891961 35.0 34.0 36.0 26.0 37.0 88-89 32.73600070994815 35.0 34.0 36.0 26.0 37.0 90-91 32.547328027738686 35.0 34.0 35.5 26.0 36.5 92-93 32.385742719862066 35.0 34.0 35.0 26.0 36.0 94-95 32.226515454049874 35.0 34.0 35.0 25.0 36.0 96-97 32.11062909646421 35.0 33.5 35.0 25.0 36.0 98-99 31.98170734606169 35.0 33.0 35.0 25.0 36.0 100-101 30.800980774350588 34.5 31.0 35.0 20.5 35.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.31191409168813067 1101 2 0.28293713318592495 1101 3 0.37233954763187427 1101 4 0.2648556985340633 1101 5 0.1944915590854066 1101 6 0.190925949215206 1101 7 0.12482884299103603 1101 8 0.21326981451178995 1101 9 0.3186513096832613 1101 10-11 0.23528550978576845 1101 12-13 0.35637110361441415 1101 14-15 0.4788099805405679 1101 16-17 0.44018325779286727 1101 18-19 0.3298590676766153 1101 20-21 0.40230767934754397 1101 22-23 0.3414437148637006 1101 24-25 0.2891214867634062 1101 26-27 0.38677313366962807 1101 28-29 0.41870817540521443 1101 30-31 0.42622204671856423 1101 32-33 0.3723013072365262 1101 34-35 0.29267680522213624 1101 36-37 0.3135991428264191 1101 38-39 0.31978414441463343 1101 40-41 0.3592423840471426 1101 42-43 0.40941565054887263 1101 44-45 0.46171122673548126 1101 46-47 0.43282074998418096 1101 48-49 0.5654174698975538 1101 50-51 0.49489499265037296 1101 52-53 0.4812841388779461 1101 54-55 0.5823330531753967 1101 56-57 0.5701542395726591 1101 58-59 0.4801273306516407 1101 60-61 0.5522618614071391 1101 62-63 0.6331734837050575 1101 64-65 0.49711376505857885 1101 66-67 0.32069629076663375 1101 68-69 0.3087518635641331 1101 70-71 0.3763922963894757 1101 72-73 0.3992385471390705 1101 74-75 0.36521079447236104 1101 76-77 0.2923390916427948 1101 78-79 0.30566036347075 1101 80-81 0.1739527121365967 1101 82-83 0.3055992590386367 1101 84-85 0.2266455439360513 1101 86-87 0.2559553571207758 1101 88-89 0.27255358399283125 1101 90-91 0.3421243470635673 1101 92-93 0.37583175281097425 1101 94-95 0.288121943703473 1101 96-97 0.19165665123832554 1101 98-99 0.18984666063003175 1101 100-101 -0.08303508989409636 1102 1 0.3002257150598808 1102 2 0.2011497491321208 1102 3 0.31919181155166854 1102 4 0.2739403084512375 1102 5 0.25236375664307786 1102 6 0.2466310199526518 1102 7 0.2468129918021944 1102 8 0.36763585625063655 1102 9 0.3751162588401584 1102 10-11 0.3796476762339722 1102 12-13 0.3192643027472073 1102 14-15 0.4672242901852144 1102 16-17 0.4178364924997524 1102 18-19 0.35211430824823253 1102 20-21 0.43872424987605285 1102 22-23 0.29377272882546634 1102 24-25 0.4891838128664361 1102 26-27 0.4902465191735317 1102 28-29 0.5085635752744935 1102 30-31 0.5307330100408194 1102 32-33 0.4035036474120446 1102 34-35 0.4003915802588338 1102 36-37 0.3845477675446958 1102 38-39 0.47949522988890436 1102 40-41 0.43843046601043056 1102 42-43 0.4150772358820589 1102 44-45 0.5283805126462084 1102 46-47 0.5971848630069374 1102 48-49 0.6597678497474746 1102 50-51 0.5651685667398567 1102 52-53 0.5388335443690764 1102 54-55 0.555831065526327 1102 56-57 0.7179470204597962 1102 58-59 0.6947786973612864 1102 60-61 0.6684902213912807 1102 62-63 0.7872631654311917 1102 64-65 0.6932235625791066 1102 66-67 0.5973156979364589 1102 68-69 0.7321425464367763 1102 70-71 0.6594420965530574 1102 72-73 0.6339699912830952 1102 74-75 0.6176614246981984 1102 76-77 0.6158606771902839 1102 78-79 0.6588489061828824 1102 80-81 0.5869265423167178 1102 82-83 0.5675555129005332 1102 84-85 0.5850309049809894 1102 86-87 0.44021783752108234 1102 88-89 0.46245755893380647 1102 90-91 0.5379153706760533 1102 92-93 0.45775352194365126 1102 94-95 0.2826951974117904 1102 96-97 0.3459972446399746 1102 98-99 0.4522739319610949 1102 100-101 0.2657870539095448 1103 1 0.4209231006971379 1103 2 0.3329371331859221 1103 3 0.5259431512354737 1103 4 0.3960011296794974 1103 5 0.4045301691240155 1103 6 0.298454906744162 1103 7 0.3428468610090505 1103 8 0.430193881435855 1103 9 0.45584564687759865 1103 10-11 0.43081318031344296 1103 12-13 0.387806110049425 1103 14-15 0.49734279907338674 1103 16-17 0.5523132449228569 1103 18-19 0.5018667896843354 1103 20-21 0.3910785931184577 1103 22-23 0.5414758898958709 1103 24-25 0.4557238033657285 1103 26-27 0.5388001606966455 1103 28-29 0.4650723967694361 1103 30-31 0.375546371042887 1103 32-33 0.48896797390319335 1103 34-35 0.5239187614640954 1103 36-37 0.5256004298277119 1103 38-39 0.4190119436424382 1103 40-41 0.4091136839184415 1103 42-43 0.6855096016428277 1103 44-45 0.6650574300816814 1103 46-47 0.5402210073844387 1103 48-49 0.6026439821240643 1103 50-51 0.5312206039759815 1103 52-53 0.5488838814776997 1103 54-55 0.6571078279501776 1103 56-57 0.5662610606794871 1103 58-59 0.5599535854778992 1103 60-61 0.49672775587303875 1103 62-63 0.5342996098311872 1103 64-65 0.6539026968475099 1103 66-67 0.5909343860047258 1103 68-69 0.5322396370519087 1103 70-71 0.5772288472260243 1103 72-73 0.5443157672162897 1103 74-75 0.4536599579215306 1103 76-77 0.44722969653339817 1103 78-79 0.5158598486702388 1103 80-81 0.4655228537067373 1103 82-83 0.4133856168249892 1103 84-85 0.5076300999206111 1103 86-87 0.5721393983048131 1103 88-89 0.3242588606981087 1103 90-91 0.49846282840204736 1103 92-93 0.6084894104686285 1103 94-95 0.5826843632658907 1103 96-97 0.37823966282133625 1103 98-99 0.4200590158423836 1103 100-101 0.5303818985228936 1104 1 0.31668007138814147 1104 2 0.36776800627536943 1104 3 0.37904876344398986 1104 4 0.30273055039308616 1104 5 0.3963198479849339 1104 6 0.3452684749297674 1104 7 0.3261647435353794 1104 8 0.352854272697833 1104 9 0.3338745993128782 1104 10-11 0.36991085318420147 1104 12-13 0.29412430159188574 1104 14-15 0.3693613875827353 1104 16-17 0.36091340180797005 1104 18-19 0.4015066670287055 1104 20-21 0.3341946643347953 1104 22-23 0.2623903399659966 1104 24-25 0.26967469356991103 1104 26-27 0.32341591101160816 1104 28-29 0.41953886851823086 1104 30-31 0.4222078082056484 1104 32-33 0.40865824464887623 1104 34-35 0.3191872489489427 1104 36-37 0.35620134736793574 1104 38-39 0.39492175847045274 1104 40-41 0.45978638828507457 1104 42-43 0.21490060214200923 1104 44-45 0.29553355264224024 1104 46-47 0.4050466509699717 1104 48-49 0.5696541321157511 1104 50-51 0.3898825640891346 1104 52-53 0.41273731274537084 1104 54-55 0.4986134496668768 1104 56-57 0.4177681086245215 1104 58-59 0.4769875059418922 1104 60-61 0.4829064147429847 1104 62-63 0.43193563635902876 1104 64-65 0.3219444853378448 1104 66-67 0.30405568672497196 1104 68-69 0.2871886493887814 1104 70-71 0.34588629094943 1104 72-73 0.3254325040058532 1104 74-75 0.47103141909246204 1104 76-77 0.45026756289580305 1104 78-79 0.34303900672010457 1104 80-81 0.29479500184432084 1104 82-83 0.405113492457879 1104 84-85 0.41524853629893954 1104 86-87 0.3182921473599336 1104 88-89 0.07217062470486013 1104 90-91 0.3415313586737412 1104 92-93 0.495079298509701 1104 94-95 0.30566327885694733 1104 96-97 0.2502428005236865 1104 98-99 0.2292890902439595 1104 100-101 0.06185400387362705 1105 1 0.11727440784333965 1105 2 0.07768158322968333 1105 3 0.2280738393328008 1105 4 0.15769294907494213 1105 5 0.07859324102810916 1105 6 0.10546761130348159 1105 7 0.06021859977431632 1105 8 0.19270968477599837 1105 9 0.1705615403962355 1105 10-11 0.2266508236486331 1105 12-13 0.20281994626050448 1105 14-15 0.26052212167370925 1105 16-17 0.3652768464046119 1105 18-19 0.4266645511356799 1105 20-21 0.34953857419865386 1105 22-23 0.3162785139134172 1105 24-25 0.3146793241465673 1105 26-27 0.34231712221164656 1105 28-29 0.5043526485619552 1105 30-31 0.4647435171585812 1105 32-33 0.4202152663379266 1105 34-35 0.5359659902434188 1105 36-37 0.5981194159384486 1105 38-39 0.46722693979658203 1105 40-41 0.5766180381254387 1105 42-43 0.4074411013466559 1105 44-45 0.5757188274947964 1105 46-47 0.6197137975249234 1105 48-49 0.561658295750739 1105 50-51 0.47880258242155094 1105 52-53 0.4900817525578063 1105 54-55 0.5797603136055969 1105 56-57 0.5257209621904124 1105 58-59 0.5492223439321293 1105 60-61 0.5074700487056134 1105 62-63 0.6496012215508458 1105 64-65 0.7065585420941289 1105 66-67 0.5292354937857198 1105 68-69 0.48830369915129523 1105 70-71 0.4185336947624734 1105 72-73 0.3837078580313431 1105 74-75 0.3108048358711173 1105 76-77 0.3975325017920426 1105 78-79 0.2811800265517448 1105 80-81 0.5523254069960402 1105 82-83 0.4403507566458771 1105 84-85 0.4176160426837825 1105 86-87 0.4540975095072852 1105 88-89 0.5477282574746667 1105 90-91 0.5297472531604726 1105 92-93 0.3551802131456938 1105 94-95 0.3050716180378359 1105 96-97 0.23512373040217227 1105 98-99 0.35559464961158227 1105 100-101 0.11274934938887426 1106 1 0.1986832876623481 1106 2 0.1852610051750645 1106 3 0.22132179347604364 1106 4 0.26532903499066407 1106 5 0.2803867788718364 1106 6 0.13048023751426285 1106 7 0.1090875787142025 1106 8 0.206189961197623 1106 9 0.31179196976952284 1106 10-11 0.15413859778112027 1106 12-13 0.16451838718010947 1106 14-15 0.29408033158497204 1106 16-17 0.3197752676150003 1106 18-19 0.304088886257901 1106 20-21 0.24573387601641627 1106 22-23 0.3048348555560949 1106 24-25 0.1993402231327721 1106 26-27 0.3150186385887679 1106 28-29 0.3618928573974287 1106 30-31 0.2764171162650939 1106 32-33 0.2567183602728278 1106 34-35 0.307421720490062 1106 36-37 0.34555783493156156 1106 38-39 0.22264758765464876 1106 40-41 0.27416136758537135 1106 42-43 0.2316119801719836 1106 44-45 0.43187487514180845 1106 46-47 0.4780041880546477 1106 48-49 0.2687433450853547 1106 50-51 0.21942407941794784 1106 52-53 0.32957859292554303 1106 54-55 0.509620172931136 1106 56-57 0.4718832100254815 1106 58-59 0.334995991901053 1106 60-61 0.3046228895003864 1106 62-63 0.27965940477256623 1106 64-65 0.3413937443803974 1106 66-67 0.22107875631646579 1106 68-69 0.24034586043008943 1106 70-71 0.2841062266975456 1106 72-73 0.1525143423604689 1106 74-75 0.39188605012251543 1106 76-77 0.34586892153245685 1106 78-79 0.2157429954153045 1106 80-81 0.15958783919431596 1106 82-83 0.13663187563610535 1106 84-85 0.2039661849596186 1106 86-87 0.2500390552839704 1106 88-89 0.17496186495760213 1106 90-91 0.11082477193554041 1106 92-93 0.2795719993336405 1106 94-95 0.3116413537291649 1106 96-97 0.2449138684076715 1106 98-99 0.16896775721973967 1106 100-101 0.27627130257129195 1107 1 0.1936071054566142 1107 2 0.0645536553684849 1107 3 0.22396711859907015 1107 4 0.2031615920286356 1107 5 0.2740185254048271 1107 6 0.18238742416724563 1107 7 0.2840375800299597 1107 8 0.2190218603581755 1107 9 0.30797891221840956 1107 10-11 0.2948260139017478 1107 12-13 0.35235225997293185 1107 14-15 0.39271293335860946 1107 16-17 0.3331860989089108 1107 18-19 0.2033944966365695 1107 20-21 0.1929165159469406 1107 22-23 0.13627021252038674 1107 24-25 0.2527151343193239 1107 26-27 0.3757880920242016 1107 28-29 0.45278487339700746 1107 30-31 0.39388310400226345 1107 32-33 0.23159928445902978 1107 34-35 0.32409299229870214 1107 36-37 0.2770622690254001 1107 38-39 0.3633175700235327 1107 40-41 0.07239134837535488 1107 42-43 0.2936113354521126 1107 44-45 0.4611329867704441 1107 46-47 0.3066050801364355 1107 48-49 0.35574401612033313 1107 50-51 0.2594375001174072 1107 52-53 0.24362296017903162 1107 54-55 0.29920794693708075 1107 56-57 0.2981644263092633 1107 58-59 0.3708855079664204 1107 60-61 0.43085575179348723 1107 62-63 0.45857081499861607 1107 64-65 0.309076502728864 1107 66-67 0.42577205386126593 1107 68-69 0.374819953217127 1107 70-71 0.4610976216608407 1107 72-73 0.4215000269477187 1107 74-75 0.49218834479897566 1107 76-77 0.46430856785755026 1107 78-79 0.33584115072701337 1107 80-81 0.30301860417418425 1107 82-83 0.2487374781202547 1107 84-85 0.35100507867294795 1107 86-87 0.3302007615548561 1107 88-89 0.27964726797330997 1107 90-91 0.36081621952902054 1107 92-93 0.2832663100096724 1107 94-95 0.2600917234562772 1107 96-97 0.2868917110960254 1107 98-99 0.3811970406596501 1107 100-101 0.32024251385520586 1108 1 0.1630410677207621 1108 2 0.13549705159489633 1108 3 0.28487906828460297 1108 4 0.1677842335380717 1108 5 0.08483613546771807 1108 6 0.06895975121127407 1108 7 0.12350298883499278 1108 8 0.21188349557829866 1108 9 0.22458268580331264 1108 10-11 0.11157129692061574 1108 12-13 0.1918176687779578 1108 14-15 0.30515004027684967 1108 16-17 0.30667666494665013 1108 18-19 0.2061146224227386 1108 20-21 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11 1621.0 12 1869.0 13 1986.0 14 2138.0 15 2347.0 16 3100.0 17 4356.0 18 5098.0 19 6293.0 20 7687.0 21 9370.0 22 9310.0 23 8034.0 24 7166.0 25 6937.0 26 7472.0 27 8384.0 28 9680.0 29 10958.0 30 13118.0 31 16331.0 32 20636.0 33 26906.0 34 38023.0 35 57405.0 36 97462.0 37 232450.0 38 477041.0 39 164446.0 40 71.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.33454623830413 26.18337474960063 14.302479270735605 22.179599741359638 2 12.589564338139153 23.522475676378154 44.553315445559306 19.33464453992339 3 13.074266824294556 35.05828639888942 32.83261122433132 19.0348355524847 4 8.856882964174389 21.215506735823553 46.92819188975467 22.99941841024739 5 8.785373901164641 42.74701311708708 39.04151921763398 9.426093764114299 6 21.772969169474386 46.75593266539672 21.505535675231936 9.965562489896957 7 22.7381429705688 34.701908249959985 29.935166094292047 12.62478268517917 8 22.904888046485055 38.06459127874314 23.988250368265536 15.042270306506275 9 26.282236688312977 16.119275251494706 26.126288179847933 31.472199880344387 10-11 20.83550752458371 30.02943429029445 33.30619308720102 15.828865097920819 12-13 21.635972342620605 29.199081785146596 31.74109003545127 17.423855836781527 14-15 21.106774299877028 29.728207127372304 28.89104672980134 20.27397184294933 16-17 18.357349548041938 32.357709276233216 30.100929905297992 19.184011270426858 18-19 18.32976509870355 31.59519896803802 32.5583846118495 17.516651321408933 20-21 19.249932252865584 31.348896949873463 31.745712913792367 17.65545788346859 22-23 19.67560971744608 30.68392152512559 30.95328272823796 18.68718602919037 24-25 19.497301610317823 31.45733644256285 30.726421874269537 18.318940072849788 26-27 18.82419454973271 31.46354054618361 31.355023310009457 18.35724159407422 28-29 18.785657008107613 29.293698059624855 33.28993743383644 18.630707498431093 30-31 19.338762158195618 31.279469310709356 31.498384190264566 17.883384340830457 32-33 18.49254827634568 31.592709362572375 31.713108045040755 18.201634316041194 34-35 18.291505026686444 31.59281145805601 31.720261413050366 18.395422102207178 36-37 19.07732095995132 30.4637482726708 31.049217789272177 19.409712978105706 38-39 18.395357442536543 32.30005158221103 31.803166719886413 17.501424255366015 40-41 17.810626085523396 31.219415180212728 32.98984837535973 17.980110358904145 42-43 18.320343343014585 31.472871963846455 30.945678954630974 19.26110573850799 44-45 19.144007533741583 30.7403675987308 31.140627333742454 18.974997533785164 46-47 18.56203608875401 30.553098090176324 31.671179906935464 19.213685914134203 48-49 18.0708132333458 31.113948704620515 31.691037571393256 19.12420049064043 50-51 18.88942726931642 30.035328409199412 30.326568889209366 20.7486754322748 52-53 18.85890889843938 30.57063667135739 30.939476920346355 19.63097750985687 54-55 19.099942633654077 30.325244995499435 30.931355303692996 19.643457067153488 56-57 19.043170552365016 30.606649108127638 30.601261108786876 19.74891923072047 58-59 18.53253876190114 30.2871724413342 30.890073720508624 20.29021507625604 60-61 19.438713092204516 32.35509451184726 29.656459577327297 18.549732818620924 62-63 18.403207799380215 31.31506779847692 31.851932848883045 18.429791553259818 64-65 18.6789585325829 29.921427988466178 31.707641754098763 19.691971724852163 66-67 19.413369972948175 30.308108733445216 30.078635713165063 20.199885580441546 68-69 18.847070169259034 30.97271149256919 30.57831920100892 19.601899137162857 70-71 19.13523510087524 29.66453891830302 32.06645674432314 19.133769236498598 72-73 19.09764481040581 30.577490523459982 30.45946956731196 19.865395098822248 74-75 18.484086385476488 30.84082899123975 30.706247860647323 19.968836762636442 76-77 18.055661202601453 30.76024670698158 32.10031345478518 19.08377863563179 78-79 18.08394819914046 30.854219754307234 30.82601199305265 20.235820053499666 80-81 18.579662389677534 31.324289605339384 29.822707428425616 20.273340576557473 82-83 19.642773653100797 30.140096377178534 30.18546386172783 20.03166610799284 84-85 17.825439009767834 30.23099896144179 30.835748116548338 21.10781391224203 86-87 17.94542612437518 31.158303070647275 31.156678494374408 19.739592310603136 88-89 19.0587399941199 29.726052256825714 31.04907507272861 20.166132676325777 90-91 18.370100345712277 30.1251498251627 32.06466503551228 19.440084793612744 92-93 19.582333880902514 30.78186698110292 30.361245097829453 19.27455404016511 94-95 18.36869185617979 29.232878036872833 31.45718379827255 20.94124630867483 96-97 19.817399325162803 29.46905305665748 31.04092607061859 19.672621547561125 98-99 18.511829524877466 29.879505660182982 31.402069126742926 20.20659568819663 100-101 18.502846741155963 29.19921561572937 31.720965875163397 20.576971767951267 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 131.0 1 100.5 2 168.5 3 277.0 4 225.0 5 218.0 6 428.5 7 845.5 8 1222.5 9 1282.0 10 1054.0 11 838.0 12 850.0 13 918.5 14 865.5 15 777.5 16 734.5 17 744.0 18 770.0 19 804.5 20 878.5 21 1001.0 22 1188.0 23 1382.5 24 1649.5 25 2322.0 26 4011.0 27 5794.0 28 6623.0 29 11548.5 30 21312.5 31 28905.0 32 41584.0 33 57161.0 34 71760.5 35 83032.0 36 99947.5 37 113599.0 38 103513.0 39 96283.0 40 85033.0 41 68248.5 42 57041.5 43 49815.0 44 48323.0 45 45474.0 46 36421.0 47 23755.5 48 17114.5 49 13334.5 50 11422.5 51 8436.0 52 4847.0 53 3587.0 54 2776.0 55 2612.5 56 2180.0 57 1705.5 58 1740.0 59 1704.0 60 1862.5 61 1893.0 62 1468.0 63 1045.0 64 603.0 65 469.5 66 379.0 67 273.0 68 227.0 69 177.5 70 137.5 71 128.0 72 110.0 73 85.0 74 86.5 75 133.0 76 128.0 77 89.0 78 111.5 79 127.5 80 107.5 81 64.5 82 30.5 83 17.0 84 14.0 85 11.5 86 6.5 87 5.0 88 5.0 89 2.0 90 2.0 91 3.0 92 2.5 93 2.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006338822753838157 2 0.00966670469960319 3 0.0015847056884595392 4 4.754117065378618E-4 5 0.0036448230834569403 6 0.005387999340762433 7 0.005546469909608388 8 0.005308764056339456 9 0.00863664600210449 10-11 0.007963146084509185 12-13 0.003961764221148848 14-15 0.0 16-17 0.0 18-19 1.5847056884595392E-4 20-21 0.0014262351196135854 22-23 3.1694113769190784E-4 24-25 0.0011885292663446545 26-27 0.004159852432206291 28-29 0.0031694113769190784 30-31 0.012519174938830362 32-33 5.546469909608388E-4 34-35 0.0 36-37 0.0 38-39 7.923528442297696E-5 40-41 0.0 42-43 1.980882110574424E-4 44-45 6.734999175953041E-4 46-47 0.0028920878814386593 48-49 0.0031694113769190784 50-51 0.0038429112945143826 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0011885292663446545 64-65 0.0029317055236501477 66-67 0.0033278819457650327 68-69 0.009706322341814677 70-71 7.923528442297696E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.015450880462480507 82-83 0.011647586810177614 84-85 0.017312909646420467 86-87 0.015569733389114972 88-89 0.014341586480558832 90-91 0.013985027700655434 92-93 0.009745939984026166 94-95 0.02551376158419858 96-97 0.010300586974987007 98-99 0.014183115911712877 100-101 0.014460439407193297 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1262064.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 12.002039359245531 #Duplication Level Percentage of deduplicated Percentage of total 1 72.87243183425898 8.746177950787134 2 9.286523038499318 2.2291443003721847 3 3.765305296129981 1.3557402709118305 4 2.0447410791894978 0.9816425164759415 5 1.395030684294888 0.8371606590131238 6 0.987470748353006 0.7110997672701849 7 0.7804223329909351 0.6556661690173997 8 0.6108224723392724 0.5864892283622091 9 0.5249023973584838 0.5669909309572974 >10 5.098811244174084 13.519412376000545 >50 1.1234636417346522 9.580974079379127 >100 1.2716422995174 31.900391581425176 >500 0.17512023192506568 14.25796392359579 >1k 0.06196562052733093 12.960506667655569 >5k 0.0013470787071158897 1.110639578776492 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 8302 0.6578113312795547 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 5437 0.43080224140772577 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 4428 0.35085383942494197 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 4016 0.3182089022426755 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3975 0.31496025558133345 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 3770 0.29871702227462316 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 3607 0.2858016709136779 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 3233 0.2561676745394845 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 3046 0.24135067635238783 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 2904 0.2300992659643251 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 2888 0.22883150141355746 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2811 0.22273038451298827 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 2766 0.2191647967139543 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 2624 0.20791338632589157 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 2616 0.2072795040505077 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 2583 0.2046647396645495 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2282 0.18081491905323344 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 2102 0.16655256785709757 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 2079 0.1647301563153691 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 2067 0.16377933290229338 No Hit CTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT 2050 0.16243233306710278 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 2044 0.16195692136056491 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 1993 0.1579159218549931 No Hit CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG 1948 0.15435033405595913 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 1923 0.1523694519453847 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 1880 0.14896233471519668 No Hit CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA 1866 0.14785304073327502 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 1820 0.1442082176498181 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 1817 0.14397051179654916 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 1776 0.14072186513520707 No Hit CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG 1753 0.13889945359347863 No Hit CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC 1723 0.1365223950607893 No Hit GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA 1721 0.13636392449194334 No Hit ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA 1706 0.1351753952255987 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 1678 0.13295680726175535 No Hit GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA 1666 0.13200598384867962 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 1658 0.1313721015732958 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 1634 0.12947045474714436 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1624 0.1286781019029146 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 1610 0.1275688079209929 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 1582 0.12535021995714954 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1554 0.1231316319933062 No Hit CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT 1551 0.12289392614003727 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 1526 0.12091304402946286 No Hit GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG 1524 0.12075457346061688 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 1513 0.11988298533196415 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1503 0.11909063248773438 No Hit ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT 1494 0.11837751492792757 No Hit ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC 1494 0.11837751492792757 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 1490 0.11806057379023568 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 1490 0.11806057379023568 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 1478 0.11710975037715995 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 1478 0.11710975037715995 No Hit TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC 1439 0.11401957428466385 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 1431 0.11338569200928003 No Hit ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG 1431 0.11338569200928003 No Hit ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC 1423 0.11275180973389623 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 1418 0.11235563331178135 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 1408 0.11156328046755158 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 1406 0.1114048098987056 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1404 0.11124633932985965 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 1383 0.10958239835697715 No Hit GATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGC 1378 0.10918622193486224 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 1369 0.10847310437505546 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 1349 0.10688839868659593 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 1333 0.10562063413582828 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 1330 0.10538292828255938 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 1321 0.10466981072275257 No Hit ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA 1320 0.1045905754383296 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 1315 0.10419439901621472 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 1292 0.10237198747448624 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 1279 0.10134192877698754 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1279 0.10134192877698754 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 1268 0.1004703406483348 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 1265 0.10023263479506585 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 7.923528442297696E-5 0.0 2 0.0 0.0 0.0 2.3770585326893088E-4 0.0 3 0.0 0.0 0.0 2.3770585326893088E-4 0.0 4 0.0 0.0 0.0 2.3770585326893088E-4 0.0 5 0.0 0.0 0.0 2.3770585326893088E-4 0.0 6 0.0 0.0 0.0 2.3770585326893088E-4 0.0 7 0.0 0.0 0.0 3.1694113769190784E-4 0.0 8 0.0 0.0 0.0 3.1694113769190784E-4 0.0 9 0.0 0.0 0.0 3.1694113769190784E-4 0.0 10-11 0.0 0.0 0.0 3.1694113769190784E-4 0.0 12-13 0.0 0.0 0.0 3.565587799033963E-4 0.0 14-15 0.0 0.0 0.0 9.508234130757235E-4 0.0 16-17 0.0 0.0 0.0 0.0017035586150940048 0.0 18-19 0.0 0.0 0.0 0.002218587963843355 0.0 20-21 0.0 0.0 0.0 0.0027732349548041935 0.0 22-23 0.0 0.0 0.0 0.005150293487493502 0.0 24-25 0.0 0.0 0.0 0.00938938120412277 0.0 26-27 0.0 0.0 0.0 0.01303420428757971 0.0 28-29 0.0 0.0 0.0 0.017233674361997492 0.0 30-31 0.0 0.0 0.0 0.025077967519872207 0.0 32-33 0.0 0.0 0.0 0.03692364254110726 0.0 34-35 0.0 0.0 0.0 0.0570890224267549 0.0 36-37 0.0 0.0 0.0 0.08196890173556967 0.0 38-39 0.0 0.0 0.0 0.12610295515916783 0.0 40-41 0.0 0.0 0.0 0.1881441828623588 0.0 42-43 0.0 0.0 0.0 0.24606517577555495 0.0 44-45 0.0 0.0 0.0 0.3024410806425031 0.0 46-47 0.0 0.0 0.0 0.36990992532866795 0.0 48-49 0.0 0.0 0.0 0.4459361807325144 0.0 50-51 0.0 0.0 0.0 0.5302821410007733 0.0 52-53 0.0 0.0 0.0 0.6170447774439332 0.0 54-55 0.0 0.0 0.0 0.7034508551071894 0.0 56-57 0.0 0.0 0.0 0.7997613433233179 0.0 58-59 0.0 0.0 0.0 0.9045103893304935 0.0 60-61 0.0 0.0 0.0 1.028473991810241 0.0 62-63 0.0 0.0 0.0 1.1574294172086361 0.0 64-65 0.0 0.0 0.0 1.293674488773945 0.0 66-67 0.0 0.0 0.0 1.4410917354428934 0.0 68-69 0.0 0.0 0.0 1.605029538914033 0.0 70-71 0.0 0.0 0.0 1.804702455659935 0.0 72-73 0.0 0.0 0.0 2.021569429125623 0.0 74-75 0.0 0.0 0.0 2.229205491956034 0.0 76-77 0.0 0.0 0.0 2.4627118751505472 0.0 78-79 0.0 0.0 0.0 2.718166432130225 0.0 80-81 0.0 0.0 0.0 3.0149025722942735 0.0 82-83 0.0 0.0 0.0 3.3419462087501106 0.0 84-85 0.0 0.0 0.0 3.647398230200687 0.0 86-87 0.0 0.0 0.0 3.9694104260956653 0.0 88-89 0.0 0.0 0.0 4.305249179122454 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 25 0.005019592 57.005157 2 CCTTTAG 260 0.0 56.639744 1 CTTTAGG 285 0.0 51.67134 2 CATTCCG 300 0.0 49.08194 9 GGCATTC 305 0.0 48.277317 7 CCGGGTC 50 2.8276289E-5 47.49865 9 ACCTGGG 190 0.0 44.998726 3 GGGATGC 180 0.0 42.221027 7 TAGGCAT 365 0.0 40.34132 5 GTTATAT 60 8.286796E-5 39.586914 1 ATATGAC 50 0.001615158 38.003437 2 GGTATAG 75 6.9185226E-6 38.003437 1 CATGGAT 100 3.0318915E-8 37.99892 4 TGGGTAT 475 0.0 37.99892 6 ATGTATT 140 1.0913936E-11 37.324806 1 GGTATCA 2770 0.0 37.043064 1 TACCTGG 245 0.0 34.90112 2 CATGGGT 805 0.0 34.81268 4 GTATCAA 4610 0.0 34.623524 1 GACATAT 485 0.0 34.281452 1 >>END_MODULE