FastQCFastQC Report
Fri 10 Feb 2017
SRR2568645_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568645_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1262064
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC79490.6298412758782439No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC54090.42858365344388244No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT46920.3717719545126079No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG44530.3528347215355164No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43840.34736748691033104No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG41330.3274794305201638No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT37730.2989547281278921No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT36880.29221972895193904No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32220.2552960864108318No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC31550.2499873223544923No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT30970.24539167585795968No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT29170.23112932466182382No Hit
TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC28510.22589979588990733No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG27870.22082873768683678No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC27520.21805550273203259No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT27470.21765932630991772No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC27250.21591615005261225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24220.1919078588724502No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT20570.1629869800580636No Hit
CTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT20450.16203615664498788No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG20430.16187768607614195No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA20390.16156074493845005No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT20140.15957986282787562No Hit
CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG20030.15870827469922286No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT19740.15641045145095653No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA19200.15213174609211577No Hit
CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA18730.14840768772423585No Hit
CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG18410.14587215862270062No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG18270.14476286464077892No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA17920.14198962968597473No Hit
CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC17820.14119727684174496No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC17750.14064262985078413No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA17750.14064262985078413No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC17420.13802786546482587No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT17190.1362054539230974No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT16980.13454151295021488RNA PCR Primer, Index 23 (95% over 23bp)
GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA16960.13438304238136892No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC16930.13414533652809998No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA16820.13327374839944725No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA16720.13248139555521748No Hit
ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA16440.13026280759137412No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC16280.1289950430406065No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA16050.12717263149887803No Hit
ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT15940.1263010433702253No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT15620.12376551426869001No Hit
GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG15520.12297316142446024No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT15390.12194310272696156No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC15360.12170539687369263No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA15360.12170539687369263No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC15340.12154692630484668No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT15130.11988298533196415No Hit
CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA15120.11980375004754117No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA15090.11956604419427223No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA15040.11916986777215735No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC14870.11782286793696674No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG14820.11742669151485186No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT14780.11710975037715995No Hit
CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT14760.116951279808314No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA14620.11584198582639232No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC14580.11552504468870042No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG14540.1152081035510085No Hit
ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC14410.1141780448535098No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC14300.11330645672485706No Hit
TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC14070.11148404518312859No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT13950.11053322177005287No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA13850.1097408689258231No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA13830.10958239835697715No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT13810.10942392778813118No Hit
ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA13730.10879004551274736No Hit
ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT13640.10807692795294058No Hit
GATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGC13580.10760151624640271No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG13140.10411516373179174No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT13130.10403592844736875No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC13090.10371898730967685No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC13020.10316434031871602No Hit
AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA12810.10150039934583349No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT12710.10070804650160373No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG12630.1000741642262199No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACGC250.005014612357.019453
ACTGTCC250.005022483456.9968649
CTTTAGG3350.049.693042
CATTCCG3550.049.5043149
GGCATTC3500.048.8718767
CCTTTAG3350.048.2828141
GGAGCGA405.3898443E-447.4973838
ATGTATT1250.045.669871
GATGGCC3300.043.179449
GTTATAT1006.2755134E-1042.81551
CTCGAGT459.6280413E-442.2199029
GTACTGG1051.0113581E-940.7766651
ACATATA4900.039.7978942
GGTATCA28150.039.20741
TACCTAT1002.9906005E-838.058221
GGGAGCG500.001613483638.011467
TGTGTAT500.001613483638.011467
TCTCGAG500.00161631337.997918
GGCGCTA500.00161631337.997918
GTATCAA49200.037.129971
Produced by FastQC (version 0.11.5)