##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568645_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1262064 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.73533909532322 34.0 31.0 34.0 31.0 34.0 2 32.842809080997476 34.0 31.0 34.0 31.0 34.0 3 32.88770775491576 34.0 31.0 34.0 31.0 34.0 4 36.29415306989186 37.0 37.0 37.0 35.0 37.0 5 36.35910857135613 37.0 37.0 37.0 35.0 37.0 6 36.3834448966138 37.0 37.0 37.0 35.0 37.0 7 36.30791227703191 37.0 37.0 37.0 35.0 37.0 8 36.30613661430799 37.0 37.0 37.0 35.0 37.0 9 38.12208255682754 39.0 39.0 39.0 37.0 39.0 10-11 38.04388525463051 39.0 39.0 39.0 36.0 39.0 12-13 38.074864269957786 39.0 39.0 39.0 36.0 39.0 14-15 39.508960322138975 41.0 39.0 41.0 37.0 41.0 16-17 39.48994226917177 41.0 39.0 41.0 37.0 41.0 18-19 39.60955823159523 41.0 40.0 41.0 37.0 41.0 20-21 39.6004303268297 41.0 40.0 41.0 37.0 41.0 22-23 39.52979959811864 41.0 39.5 41.0 37.0 41.0 24-25 39.51252353287947 41.0 39.0 41.0 37.0 41.0 26-27 39.41361888145134 41.0 39.0 41.0 36.0 41.0 28-29 39.31169417715742 41.0 39.0 41.0 36.0 41.0 30-31 39.23368783199584 41.0 39.0 41.0 36.0 41.0 32-33 39.070013882021826 41.0 39.0 41.0 35.0 41.0 34-35 38.98583312732159 41.0 39.0 41.0 35.0 41.0 36-37 38.88629300891239 41.0 39.0 41.0 35.0 41.0 38-39 38.721078328832775 40.0 38.0 41.0 35.0 41.0 40-41 38.68455165506663 40.0 38.0 41.0 35.0 41.0 42-43 38.56693638357484 40.0 38.0 41.0 35.0 41.0 44-45 38.44744798995931 40.0 38.0 41.0 34.5 41.0 46-47 38.229261749007975 40.0 38.0 41.0 34.0 41.0 48-49 38.09999057100116 40.0 38.0 41.0 33.5 41.0 50-51 38.073740713624666 40.0 38.0 41.0 33.5 41.0 52-53 38.18563083964046 40.0 38.0 41.0 34.0 41.0 54-55 38.081500621204626 40.0 38.0 41.0 34.0 41.0 56-57 37.95362279567439 40.0 38.0 41.0 33.0 41.0 58-59 37.79417129400728 40.0 38.0 41.0 33.0 41.0 60-61 37.76473657437341 40.0 38.0 41.0 33.0 41.0 62-63 37.59614409411884 40.0 37.5 41.0 33.0 41.0 64-65 37.36923285982327 40.0 37.0 41.0 32.5 41.0 66-67 37.12236978473358 39.0 37.0 41.0 32.0 41.0 68-69 36.765102245211025 39.0 36.0 41.0 32.0 41.0 70-71 36.374261130972755 39.0 36.0 40.5 31.0 41.0 72-73 35.975272648613696 37.5 35.0 40.0 31.0 41.0 74-75 35.54371727582753 37.0 35.0 39.0 31.0 41.0 76-77 33.73820662026648 35.5 33.0 38.0 28.0 39.0 78-79 34.63347104425766 36.0 35.0 38.5 30.0 40.0 80-81 34.451691831793 36.0 35.0 37.0 30.0 39.0 82-83 34.14303196985256 35.5 35.0 37.0 30.0 39.0 84-85 33.74030041265736 35.0 35.0 36.5 29.5 38.0 86-87 33.50523705612393 35.0 34.0 36.0 29.0 37.0 88-89 33.27048311337619 35.0 34.0 36.0 29.0 37.0 90-91 33.12942687534071 35.0 34.0 36.0 29.0 36.5 92-93 32.99542693555953 35.0 34.0 35.0 29.0 36.0 94-95 32.86892820015467 35.0 34.0 35.0 29.0 36.0 96-97 32.763779808314 35.0 34.0 35.0 29.0 36.0 98-99 32.6938273336376 35.0 34.0 35.0 29.0 36.0 100-101 31.721495502605258 34.5 32.5 35.0 24.5 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32403772749493953 1101 2 0.20834789635503626 1101 3 0.2484339059826297 1101 4 0.13878073408801583 1101 5 0.2785349336563385 1101 6 0.26102154133626243 1101 7 0.3260495799354928 1101 8 0.2205938753101151 1101 9 0.2670916017998479 1101 10-11 0.3345462298796136 1101 12-13 0.306775736361935 1101 14-15 0.3714089898867883 1101 16-17 0.4876629712235001 1101 18-19 0.4757197071015611 1101 20-21 0.4607913740422305 1101 22-23 0.44007904703741474 1101 24-25 0.5380216231749486 1101 26-27 0.44952741878398683 1101 28-29 0.44756627636296287 1101 30-31 0.3714558125294545 1101 32-33 0.4463770305613508 1101 34-35 0.5019341519119536 1101 36-37 0.4916593194878516 1101 38-39 0.4224397792820298 1101 40-41 0.34489673872503346 1101 42-43 0.47938205415425017 1101 44-45 0.40241110770515576 1101 46-47 0.24078302549440167 1101 48-49 0.3663776333213846 1101 50-51 0.3691268306862341 1101 52-53 0.4090696373017124 1101 54-55 0.535657054869553 1101 56-57 0.5142899479261018 1101 58-59 0.6305037482071612 1101 60-61 0.5723423771610925 1101 62-63 0.4993214814347908 1101 64-65 0.37725556463951904 1101 66-67 0.4198810313599779 1101 68-69 0.45842479699776817 1101 70-71 0.31428158874255985 1101 72-73 0.3173236951999101 1101 74-75 0.33446726818652195 1101 76-77 0.24745265580172315 1101 78-79 0.29538173933044476 1101 80-81 0.42277122617716856 1101 82-83 0.23282435826140357 1101 84-85 0.3031905936968613 1101 86-87 0.46286850487137343 1101 88-89 0.20614301462051543 1101 90-91 0.22962768034544467 1101 92-93 0.2654307475278159 1101 94-95 0.2941795570932797 1101 96-97 0.25255110430240535 1101 98-99 0.2998844232996092 1101 100-101 0.2918000629052351 1102 1 0.4359354055350835 1102 2 0.2159940055275129 1102 3 0.22048806004418253 1102 4 0.14925651977193866 1102 5 0.1963109097116842 1102 6 0.1720005790498078 1102 7 0.15143916272563018 1102 8 0.21772044551716618 1102 9 0.13591033463338675 1102 10-11 0.26423827821180623 1102 12-13 0.2304950153078522 1102 14-15 0.34725449972859224 1102 16-17 0.36280048153677313 1102 18-19 0.33650256938766177 1102 20-21 0.29882833395707564 1102 22-23 0.3482569818110903 1102 24-25 0.3156473558191877 1102 26-27 0.3228705980938571 1102 28-29 0.32689785123108095 1102 30-31 0.3028667397775635 1102 32-33 0.41551176923817223 1102 34-35 0.49405365016003344 1102 36-37 0.33363184242707433 1102 38-39 0.37899053844610364 1102 40-41 0.3160829705495587 1102 42-43 0.436150686801696 1102 44-45 0.47858601725194916 1102 46-47 0.5444290132291982 1102 48-49 0.4641550559137926 1102 50-51 0.38250104803824314 1102 52-53 0.5356702037727672 1102 54-55 0.546444024606572 1102 56-57 0.6566524108251102 1102 58-59 0.6778836409848452 1102 60-61 0.6713618571935243 1102 62-63 0.6166316189808185 1102 64-65 0.5931311303069364 1102 66-67 0.474505663921704 1102 68-69 0.3258501217542644 1102 70-71 0.41146432387626675 1102 72-73 0.5583792743683418 1102 74-75 0.49514539047425643 1102 76-77 0.4390246129842268 1102 78-79 0.3920104150525603 1102 80-81 0.4030197448160635 1102 82-83 0.3063642487531908 1102 84-85 0.37518885070898733 1102 86-87 0.40147282876709056 1102 88-89 0.39150021998234763 1102 90-91 0.3383398516154372 1102 92-93 0.32205374425257105 1102 94-95 0.364574122399965 1102 96-97 0.40953430661401313 1102 98-99 0.4579927099925456 1102 100-101 0.3194936577819938 1103 1 0.3706271740843903 1103 2 0.23196436997151437 1103 3 0.29573120327992086 1103 4 0.22796992327720744 1103 5 0.20092875605016047 1103 6 0.20272038303509987 1103 7 0.28377158765750465 1103 8 0.26628242099865673 1103 9 0.2701804048886558 1103 10-11 0.33673413206751235 1103 12-13 0.2874063669925633 1103 14-15 0.45287617135396374 1103 16-17 0.4595098180703445 1103 18-19 0.3539372103190601 1103 20-21 0.31587502912587695 1103 22-23 0.3557804627388208 1103 24-25 0.3217732319265636 1103 26-27 0.28427645353302466 1103 28-29 0.4189626727593563 1103 30-31 0.38715722823086907 1103 32-33 0.3696395788239002 1103 34-35 0.3821465071243111 1103 36-37 0.44053319336172336 1103 38-39 0.5513651332073835 1103 40-41 0.4208941647224549 1103 42-43 0.42375142352361905 1103 44-45 0.5949143252083786 1103 46-47 0.6455449302563139 1103 48-49 0.6348782768220289 1103 50-51 0.6135283750877747 1103 52-53 0.7139602421923144 1103 54-55 0.6977870419995398 1103 56-57 0.7424109260470857 1103 58-59 0.6728782655816872 1103 60-61 0.6541634839821953 1103 62-63 0.6340383411516441 1103 64-65 0.6351384475224009 1103 66-67 0.6971011085800498 1103 68-69 0.6535663671393408 1103 70-71 0.6635415630025321 1103 72-73 0.7033597312359419 1103 74-75 0.632601116320366 1103 76-77 0.6452325785816413 1103 78-79 0.545896539845252 1103 80-81 0.6497982532041888 1103 82-83 0.580346880783928 1103 84-85 0.6375535280597902 1103 86-87 0.562482404485273 1103 88-89 0.685261418738925 1103 90-91 0.631526007243771 1103 92-93 0.6244397565368303 1103 94-95 0.49842666134038893 1103 96-97 0.5814442831955873 1103 98-99 0.5684172418324351 1103 100-101 0.6328875789927473 1104 1 0.2472489145583694 1104 2 0.07762982434409338 1104 3 0.16978335569618963 1104 4 0.14018319195485418 1104 5 0.12983365039040962 1104 6 0.05358681675114241 1104 7 0.11955803373418661 1104 8 0.08132437588230346 1104 9 0.09342792681691492 1104 10-11 0.23668064850479453 1104 12-13 0.2081163910007362 1104 14-15 0.24815654407497334 1104 16-17 0.3135769458841793 1104 18-19 0.09746569923013482 1104 20-21 0.2119731060946748 1104 22-23 0.2608114832052024 1104 24-25 0.30234205932915614 1104 26-27 0.27707518894389693 1104 28-29 0.28932921344252094 1104 30-31 0.32558219673499167 1104 32-33 0.220521463346941 1104 34-35 0.18858711659922278 1104 36-37 0.34764294163242226 1104 38-39 0.3482321849557053 1104 40-41 0.3801373128838108 1104 42-43 0.42354224336720137 1104 44-45 0.32975114091590996 1104 46-47 0.3748467914842948 1104 48-49 0.41514997332063786 1104 50-51 0.4475439209766705 1104 52-53 0.5404167778836779 1104 54-55 0.5388766804506631 1104 56-57 0.4269113895712948 1104 58-59 0.47295433109310636 1104 60-61 0.558222292130715 1104 62-63 0.5310919911076724 1104 64-65 0.4540074484494525 1104 66-67 0.5374217722516406 1104 68-69 0.4088374693761452 1104 70-71 0.47286671929338553 1104 72-73 0.4410252331494675 1104 74-75 0.40970420885881964 1104 76-77 0.4462998727887779 1104 78-79 0.5061511216037857 1104 80-81 0.4963764937138322 1104 82-83 0.5022596906914671 1104 84-85 0.5223119012086741 1104 86-87 0.5587789648979253 1104 88-89 0.4694183989381173 1104 90-91 0.40290635857135015 1104 92-93 0.4157611570930584 1104 94-95 0.5477171127416582 1104 96-97 0.5006520133531822 1104 98-99 0.5178443734495417 1104 100-101 0.6751502902528266 1105 1 0.29744643691935124 1105 2 0.11294908383159452 1105 3 0.20437242546383771 1105 4 0.1327069468562314 1105 5 0.13357867780705135 1105 6 0.10932463203385367 1105 7 0.14277219768853655 1105 8 0.1366729292694444 1105 9 0.2577516856885893 1105 10-11 0.23431832077044845 1105 12-13 0.22959354926806697 1105 14-15 0.3190022090697937 1105 16-17 0.34021117693657743 1105 18-19 0.30982489388093626 1105 20-21 0.25119361562208553 1105 22-23 0.2923615482404216 1105 24-25 0.2714328522927545 1105 26-27 0.25505729325399784 1105 28-29 0.2267184658439092 1105 30-31 0.22316143656088627 1105 32-33 0.4259149415271324 1105 34-35 0.3013850292578226 1105 36-37 0.40860558371085176 1105 38-39 0.5007184119031507 1105 40-41 0.592449036571729 1105 42-43 0.6200638301402464 1105 44-45 0.6152915719349821 1105 46-47 0.8309236357527752 1105 48-49 0.7522274709621897 1105 50-51 0.7478253806311059 1105 52-53 0.755377812854249 1105 54-55 0.7288261281907396 1105 56-57 0.780221798745103 1105 58-59 0.8185377621734631 1105 60-61 0.7398228921636871 1105 62-63 0.7903844320904767 1105 64-65 0.7210644127417893 1105 66-67 0.6266995490224545 1105 68-69 0.587501774578179 1105 70-71 0.6330488524313154 1105 72-73 0.5790535310219909 1105 74-75 0.6352353010448368 1105 76-77 0.42793422036081097 1105 78-79 0.5825642674354512 1105 80-81 0.5386903838038819 1105 82-83 0.36302951434978326 1105 84-85 0.511802261582325 1105 86-87 0.42786181726409467 1105 88-89 0.4776006667209529 1105 90-91 0.44992418952811164 1105 92-93 0.4831919336403274 1105 94-95 0.5323947805881986 1105 96-97 0.43336278658612315 1105 98-99 0.4355935938468676 1105 100-101 0.5629280709076276 1106 1 0.2011899544379645 1106 2 0.05878407670753916 1106 3 0.09696381664642928 1106 4 0.12199909889633886 1106 5 0.10816611893606165 1106 6 0.08376753897765354 1106 7 0.11004095325741048 1106 8 0.05765789689296241 1106 9 0.15831791478348833 1106 10-11 0.12248745861581511 1106 12-13 0.21241861773702908 1106 14-15 0.1801463285404381 1106 16-17 0.1734511652752957 1106 18-19 0.046477449302813056 1106 20-21 0.2202419483463558 1106 22-23 0.1631327941497318 1106 24-25 0.18633465527459236 1106 26-27 0.1389807394088507 1106 28-29 0.14377514213250464 1106 30-31 0.11119507360762526 1106 32-33 0.10083374370007192 1106 34-35 0.06790360313245714 1106 36-37 0.1380265025705114 1106 38-39 0.24066363673182423 1106 40-41 0.1031211615937977 1106 42-43 0.11660075821395566 1106 44-45 0.02170199153579233 1106 46-47 0.1642301426653674 1106 48-49 0.28740758437225367 1106 50-51 0.22294825521895234 1106 52-53 0.23918546741754199 1106 54-55 0.1501071445288531 1106 56-57 0.13712802808636582 1106 58-59 0.1610497006150453 1106 60-61 0.25017351074536265 1106 62-63 0.3265766954054854 1106 64-65 0.18273164741685122 1106 66-67 0.25123997548042354 1106 68-69 0.24834943472575333 1106 70-71 0.22520992592612998 1106 72-73 0.302668035355957 1106 74-75 0.21983584058437344 1106 76-77 0.1809105159332205 1106 78-79 0.3467427835785202 1106 80-81 0.45322469219548367 1106 82-83 0.2159663915080472 1106 84-85 0.19086122906623615 1106 86-87 0.17117854832785184 1106 88-89 0.16743564317255988 1106 90-91 0.10970837371066011 1106 92-93 0.18133598330753387 1106 94-95 0.17452074705790466 1106 96-97 0.23191169621529895 1106 98-99 0.2117113727834692 1106 100-101 0.333816750842427 1107 1 0.2002761626956442 1107 2 -0.0061411884170610165 1107 3 0.09744801443069662 1107 4 0.0849816519870501 1107 5 0.04368755258253998 1107 6 0.04282322172284836 1107 7 0.09567027880147805 1107 8 0.08170397462775725 1107 9 0.05571074655107111 1107 10-11 0.0898277917649466 1107 12-13 0.19234644858358507 1107 14-15 0.28259527725231237 1107 16-17 0.18942015293350778 1107 18-19 0.11089581987201313 1107 20-21 0.12969663374937568 1107 22-23 0.12042010237846057 1107 24-25 0.1460189398986813 1107 26-27 0.24329608637340527 1107 28-29 0.16199768890758293 1107 30-31 0.031367240129561935 1107 32-33 0.04433786201274614 1107 34-35 0.06928317209116841 1107 36-37 0.25012651309655354 1107 38-39 0.27677946263113995 1107 40-41 0.3520708602859415 1107 42-43 0.21940627648978506 1107 44-45 0.270666492469978 1107 46-47 0.24562142184223745 1107 48-49 0.3644486490810763 1107 50-51 0.31805200248876275 1107 52-53 0.32644961836093955 1107 54-55 0.37814357778060526 1107 56-57 0.3671453302626162 1107 58-59 0.3180189251091363 1107 60-61 0.3687776264265281 1107 62-63 0.3578590958686263 1107 64-65 0.36220159557611 1107 66-67 0.40517309566147475 1107 68-69 0.2903269484753892 1107 70-71 0.26945053801528474 1107 72-73 0.27517895612120213 1107 74-75 0.38369962048491146 1107 76-77 0.16518625867683312 1107 78-79 0.3622147448426958 1107 80-81 0.3639152003400099 1107 82-83 0.38167485970624426 1107 84-85 0.3764070041774161 1107 86-87 0.2474156868242119 1107 88-89 0.33307503424121165 1107 90-91 0.34642911790160014 1107 92-93 0.27923110448855937 1107 94-95 0.24352057600977872 1107 96-97 0.41779265987414504 1107 98-99 0.31526621344556105 1107 100-101 0.33242862947719587 1108 1 0.10640823816734013 1108 2 0.04124874868985273 1108 3 0.05317128487094891 1108 4 0.08083070859081687 1108 5 0.06953660918631499 1108 6 0.019395548766873105 1108 7 0.1154187064744292 1108 8 0.06947127022524313 1108 9 0.13335225598503087 1108 10-11 0.1728466596894762 1108 12-13 0.14075839826912073 1108 14-15 0.13959213259822434 1108 16-17 0.20412141079514612 1108 18-19 0.07896185760785812 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-0.4409363913152049 2316 94-95 -0.4425107390825431 2316 96-97 -0.4601434460127223 2316 98-99 -0.5318173979520466 2316 100-101 -0.44108044047366235 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 2.0 12 2.0 13 25.0 14 82.0 15 316.0 16 947.0 17 2129.0 18 3346.0 19 4346.0 20 5589.0 21 7800.0 22 8412.0 23 6627.0 24 5519.0 25 5861.0 26 7123.0 27 8813.0 28 10404.0 29 11804.0 30 13154.0 31 15698.0 32 19681.0 33 24861.0 34 33419.0 35 51026.0 36 87381.0 37 220131.0 38 513756.0 39 193695.0 40 114.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.73515156702201 26.264220800010147 14.434528054876678 22.566099578091173 2 12.52966671743324 23.484697050461975 44.63540080211189 19.350235429992892 3 12.752443616171604 34.981744190468945 33.42857414520975 18.8372380481497 4 8.760173810519909 21.528781424713802 46.64985293931211 23.061191825454177 5 8.719288403757654 42.557984381140734 39.32336236514155 9.399364849960065 6 21.546682894824425 46.979407355285716 21.419187260896162 10.054722488993697 7 22.39116134196698 34.78018276493386 29.70025282746705 13.128403065632108 8 22.650277640436617 38.30035560795649 23.97493312541995 15.074433626186945 9 25.85352248380431 15.850464001825582 25.913582829396926 32.38243068497319 10-11 20.593488127384983 30.03738320719076 33.37303021082925 15.996098454595012 12-13 21.496136487531537 28.941638458905413 31.83986707488685 17.7223579786762 14-15 20.9339225269083 29.625755904613392 28.777304479012155 20.663017089466145 16-17 18.33987816782667 32.465231557195196 29.98663300751784 19.208257267460286 18-19 18.28631511555674 31.38010433702253 32.56150242776911 17.772078119651617 20-21 19.168164213542262 31.08693378465751 31.76566323102473 17.979238770775492 22-23 19.439862003828647 30.72692826988172 31.077900962233297 18.755308764056338 24-25 19.467911294514384 31.494916264151424 30.93183071539954 18.10534172593466 26-27 18.853124722676505 31.554342727468654 31.427368184180832 18.16516436567401 28-29 18.61729670666642 29.055278735105084 33.465763426870346 18.861661131358158 30-31 19.446042356013642 31.192831742288824 31.59304916390928 17.768076737788256 32-33 18.673022921183076 31.51218955615563 31.916487594923872 17.898299927737423 34-35 18.318207317536984 31.513219614853128 31.950717237794596 18.217855829815285 36-37 19.154345244910417 30.257471196972258 31.241098407489005 19.347085150628317 38-39 18.324704610859673 31.92524309385261 32.17023859328845 17.579813701999264 40-41 17.667368691286654 30.841581726441763 33.39949479582652 18.09155478644506 42-43 18.39625407269362 31.000250383498777 31.413026597700277 19.190468946107327 44-45 18.95755682754599 30.556215849592416 31.5496678456877 18.9365594771739 46-47 18.685740184332968 30.173271719976164 31.757422761444744 19.383565334246125 48-49 18.080310539049737 30.715372087062182 31.880329317819587 19.323988056068494 50-51 18.81120925721675 29.687123632398993 30.550312820902903 20.95135428948136 52-53 18.715215710138313 30.32643352476578 31.38501692465675 19.57333384043915 54-55 18.99222226448104 30.15207628139302 31.095055401310866 19.76064605281507 56-57 19.029819406939744 30.561326525437693 30.599161373749666 19.809692693872893 58-59 18.234931033608436 30.5089123847919 31.08336819685848 20.172788384741185 60-61 19.414189771675606 32.79576154616565 29.40211431432954 18.387934367829207 62-63 18.218298841540065 31.90287176516871 31.657995021645107 18.220834371646117 64-65 18.495799920685197 30.530295917110088 31.446615631038 19.527288531166718 66-67 19.19930368032049 30.876999898578838 29.855498611797813 20.068197809302855 68-69 18.596085460008368 31.431211095475348 30.3530565803319 19.619646864184386 70-71 18.980852001166344 30.09934519960953 31.854089808440776 19.06571299078335 72-73 18.950029475525806 30.952907301056047 30.142132253197936 19.95493097022021 74-75 18.430206392068865 31.12013336883074 30.37619328338341 20.073466955716984 76-77 18.014458854701505 31.020376145742212 31.863598042571535 19.10156695698475 78-79 18.00122656220287 31.07718784467349 30.597695520987845 20.323890072135804 80-81 18.4689524461517 31.5882950468439 29.587406026952674 20.355346480051725 82-83 19.529120551731133 30.438630687508716 29.936794013615792 20.09545474714436 84-85 17.72322956680485 30.49144100457663 30.549678938627515 21.235650489991 86-87 17.850837992368056 31.500621204629876 30.827358992887845 19.821181810114226 88-89 19.060800403149127 30.002321593833592 30.666352894940353 20.270525108076928 90-91 18.399581954639384 30.398933810012807 31.611867543959736 19.589616691388077 92-93 19.541441638458906 31.093946107328946 29.88053696167548 19.48407529253667 94-95 18.333024315724085 29.586019409475274 30.88056548637787 21.200390788422773 96-97 19.74258040796663 29.81802032226575 30.524957529887548 19.914441739880072 98-99 18.459721535516422 30.210987715361505 30.85794381267511 20.471346936446963 100-101 18.357066479352895 29.717242573314046 31.14150866029055 20.78418228704251 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 122.0 1 99.5 2 185.0 3 286.0 4 220.0 5 200.0 6 401.5 7 824.5 8 1221.0 9 1282.0 10 1018.0 11 796.0 12 796.0 13 810.5 14 768.5 15 732.0 16 687.5 17 674.0 18 724.0 19 766.5 20 838.5 21 983.0 22 1149.5 23 1352.5 24 1597.5 25 2189.5 26 3784.0 27 5499.5 28 6350.5 29 11094.0 30 20602.0 31 28149.0 32 41126.5 33 57048.0 34 71045.5 35 82129.0 36 99843.5 37 114198.5 38 104443.0 39 97269.5 40 86194.5 41 69178.0 42 57726.0 43 50791.5 44 49734.0 45 46551.5 46 37009.5 47 24403.0 48 17775.0 49 13674.0 50 11602.0 51 8693.0 52 4925.5 53 3506.5 54 2550.5 55 2281.0 56 1883.5 57 1420.5 58 1391.5 59 1425.5 60 1487.5 61 1434.0 62 1059.0 63 653.5 64 317.0 65 215.0 66 140.0 67 94.0 68 68.0 69 39.5 70 32.0 71 33.5 72 26.0 73 13.0 74 19.0 75 80.5 76 82.0 77 36.5 78 84.5 79 106.0 80 55.0 81 17.0 82 6.5 83 1.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014262351196135854 2 0.11029551591678394 3 0.0 4 0.0 5 0.0 6 0.004754117065378618 7 0.026464584997274304 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.001148911624133166 30-31 0.0 32-33 0.0 34-35 0.0 36-37 3.961764221148848E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 3.961764221148848E-5 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 3.961764221148848E-5 64-65 3.5655877990339635E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 8.319704864412582E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1262064.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 11.04346920516929 #Duplication Level Percentage of deduplicated Percentage of total 1 70.62946164158123 7.79994284616488 2 10.199717451282208 2.252805311493257 3 4.008544793257565 1.3280472294564485 4 2.1855911938767134 0.9654603617866665 5 1.4430015929111653 0.7967871827162344 6 1.0464293444721764 0.693372614463838 7 0.851336945138597 0.6581199335802695 8 0.6705395092784631 0.5924065937252827 9 0.5371867459703774 0.5339164757894065 >10 5.563065257323741 13.537363605002529 >50 1.198748830027104 9.435519042825149 >100 1.4043313588481776 32.132167259750524 >500 0.18820935737140523 14.120662349974634 >1k 0.0723882143736174 14.085567942961157 >5k 0.0014477642874723478 1.0678612503097378 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 7949 0.6298412758782439 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 5409 0.42858365344388244 No Hit CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 4692 0.3717719545126079 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 4453 0.3528347215355164 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4384 0.34736748691033104 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 4133 0.3274794305201638 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 3773 0.2989547281278921 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 3688 0.29221972895193904 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3222 0.2552960864108318 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 3155 0.2499873223544923 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 3097 0.24539167585795968 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 2917 0.23112932466182382 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 2851 0.22589979588990733 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 2787 0.22082873768683678 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 2752 0.21805550273203259 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 2747 0.21765932630991772 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 2725 0.21591615005261225 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2422 0.1919078588724502 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 2057 0.1629869800580636 No Hit CTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT 2045 0.16203615664498788 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 2043 0.16187768607614195 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 2039 0.16156074493845005 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 2014 0.15957986282787562 No Hit CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG 2003 0.15870827469922286 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 1974 0.15641045145095653 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1920 0.15213174609211577 No Hit CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA 1873 0.14840768772423585 No Hit CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG 1841 0.14587215862270062 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 1827 0.14476286464077892 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 1792 0.14198962968597473 No Hit CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC 1782 0.14119727684174496 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 1775 0.14064262985078413 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 1775 0.14064262985078413 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 1742 0.13802786546482587 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 1719 0.1362054539230974 No Hit ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT 1698 0.13454151295021488 RNA PCR Primer, Index 23 (95% over 23bp) GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA 1696 0.13438304238136892 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 1693 0.13414533652809998 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 1682 0.13327374839944725 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1672 0.13248139555521748 No Hit ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA 1644 0.13026280759137412 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 1628 0.1289950430406065 No Hit GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA 1605 0.12717263149887803 No Hit ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT 1594 0.1263010433702253 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 1562 0.12376551426869001 No Hit GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG 1552 0.12297316142446024 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 1539 0.12194310272696156 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 1536 0.12170539687369263 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1536 0.12170539687369263 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 1534 0.12154692630484668 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 1513 0.11988298533196415 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 1512 0.11980375004754117 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1509 0.11956604419427223 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 1504 0.11916986777215735 No Hit ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC 1487 0.11782286793696674 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 1482 0.11742669151485186 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 1478 0.11710975037715995 No Hit CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT 1476 0.116951279808314 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 1462 0.11584198582639232 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 1458 0.11552504468870042 No Hit ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG 1454 0.1152081035510085 No Hit ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC 1441 0.1141780448535098 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 1430 0.11330645672485706 No Hit TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC 1407 0.11148404518312859 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1395 0.11053322177005287 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 1385 0.1097408689258231 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 1383 0.10958239835697715 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 1381 0.10942392778813118 No Hit ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA 1373 0.10879004551274736 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 1364 0.10807692795294058 No Hit GATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGC 1358 0.10760151624640271 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 1314 0.10411516373179174 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 1313 0.10403592844736875 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 1309 0.10371898730967685 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 1302 0.10316434031871602 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 1281 0.10150039934583349 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1271 0.10070804650160373 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 1263 0.1000741642262199 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.5847056884595392E-4 0.0 3 0.0 0.0 0.0 1.5847056884595392E-4 0.0 4 0.0 0.0 0.0 1.5847056884595392E-4 0.0 5 0.0 0.0 0.0 1.5847056884595392E-4 0.0 6 0.0 0.0 0.0 1.5847056884595392E-4 0.0 7 0.0 0.0 0.0 2.3770585326893088E-4 0.0 8 0.0 0.0 0.0 2.3770585326893088E-4 0.0 9 0.0 0.0 0.0 2.3770585326893088E-4 0.0 10-11 0.0 0.0 0.0 2.3770585326893088E-4 0.0 12-13 0.0 0.0 0.0 2.773234954804194E-4 0.0 14-15 0.0 0.0 0.0 8.715881286527466E-4 0.0 16-17 0.0 0.0 0.0 0.0016243233306710279 0.0 18-19 0.0 0.0 0.0 0.0021789703216318667 0.0 20-21 0.0 0.0 0.0 0.002654382028169728 0.0 22-23 0.0 0.0 0.0 0.005031440560859037 0.0 24-25 0.0 0.0 0.0 0.009310145919699794 0.0 26-27 0.0 0.0 0.0 0.012954969003156734 0.0 28-29 0.0 0.0 0.0 0.017194056719786 0.0 30-31 0.0 0.0 0.0 0.024721408739968812 0.0 32-33 0.0 0.0 0.0 0.036804789614472797 0.0 34-35 0.0 0.0 0.0 0.05697016950012044 0.0 36-37 0.0 0.0 0.0 0.08177081352451224 0.0 38-39 0.0 0.0 0.0 0.1269349256456091 0.0 40-41 0.0 0.0 0.0 0.18937232977091495 0.0 42-43 0.0 0.0 0.0 0.24721408739968814 0.0 44-45 0.0 0.0 0.0 0.3037088451932707 0.0 46-47 0.0 0.0 0.0 0.3709003663839552 0.0 48-49 0.0 0.0 0.0 0.4473227982099165 0.0 50-51 0.0 0.0 0.0 0.5314310526249064 0.0 52-53 0.0 0.0 0.0 0.6179559832147974 0.0 54-55 0.0 0.0 0.0 0.7047582373001686 0.0 56-57 0.0 0.0 0.0 0.8010291078740857 0.0 58-59 0.0 0.0 0.0 0.906491271441068 0.0 60-61 0.0 0.0 0.0 1.0308114327007187 0.0 62-63 0.0 0.0 0.0 1.1595291522458449 0.0 64-65 0.0 0.0 0.0 1.2964873413709606 0.0 66-67 0.0 0.0 0.0 1.444142293893178 0.0 68-69 0.0 0.0 0.0 1.60867436199749 0.0 70-71 0.0 0.0 0.0 1.80910001394541 0.0 72-73 0.0 0.0 0.0 2.0257292815578287 0.0 74-75 0.0 0.0 0.0 2.2339992266636237 0.0 76-77 0.0 0.0 0.0 2.4678621686380406 0.0 78-79 0.0 0.0 0.0 2.7241486961041597 0.0 80-81 0.0 0.0 0.0 3.0220337478923414 0.0 82-83 0.0 0.0 0.0 3.3492358549170245 0.0 84-85 0.0 0.0 0.0 3.6556386997806767 0.0 86-87 0.0 0.0 0.0 3.978562101446519 0.0 88-89 0.0 0.0 0.0 4.316777913005996 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCACGC 25 0.0050146123 57.01945 3 ACTGTCC 25 0.0050224834 56.996864 9 CTTTAGG 335 0.0 49.69304 2 CATTCCG 355 0.0 49.504314 9 GGCATTC 350 0.0 48.871876 7 CCTTTAG 335 0.0 48.282814 1 GGAGCGA 40 5.3898443E-4 47.497383 8 ATGTATT 125 0.0 45.66987 1 GATGGCC 330 0.0 43.17944 9 GTTATAT 100 6.2755134E-10 42.8155 1 CTCGAGT 45 9.6280413E-4 42.219902 9 GTACTGG 105 1.0113581E-9 40.776665 1 ACATATA 490 0.0 39.797894 2 GGTATCA 2815 0.0 39.2074 1 TACCTAT 100 2.9906005E-8 38.05822 1 GGGAGCG 50 0.0016134836 38.01146 7 TGTGTAT 50 0.0016134836 38.01146 7 TCTCGAG 50 0.001616313 37.99791 8 GGCGCTA 50 0.001616313 37.99791 8 GTATCAA 4920 0.0 37.12997 1 >>END_MODULE