FastQCFastQC Report
Fri 10 Feb 2017
SRR2568643_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568643_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1487102
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21450.041.640811
GGTATCA12550.037.8568881
TCAACGC23850.036.8442544
GTACATG53050.036.360451
ATCAACG25350.034.8514943
TACATGG54350.034.2657742
CAACGCA25900.034.1114045
AACGCAG26950.032.9586376
ACATGGG56300.032.5660673
TATCAAC27900.032.1833762
CATGGGG38250.028.5616154
GAGTACA36100.026.3215471
ACGCAGA34200.025.971797
CGCAGAG36050.024.507228
AGTACAT36850.023.335392
GGGCGTA1452.335241E-522.9306559
GTCCTAC4550.021.9278741
GTAATAC3550.021.4080943
GCAGAGT42200.020.8231129
ATGGGGG23400.020.7047845
Produced by FastQC (version 0.11.5)