FastQCFastQC Report
Fri 10 Feb 2017
SRR2568643_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568643_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1487102
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13700.047.2209741
GTATCAA22500.041.861
TCAACGC24750.037.2406274
CAACGCA26100.035.314395
ATCAACG26850.034.5048983
GTACATG46950.032.6239741
TATCAAC28350.032.545592
AACGCAG28500.032.5072486
TACATGG48550.030.6618672
ACATGGG47050.030.0917263
TATAGCG700.00834067727.1772442
ACGCAGA35500.026.0964897
CGCAGAG35550.025.9200448
GAGTACA32300.024.4468081
CATGGGG32250.023.7184684
AGTACAT31550.022.6118132
GCAGAGT44150.020.7634859
GGACCGT2401.4105717E-719.7960826
GTACTAT4850.018.634961
GAGTACT22600.018.5998112-13
Produced by FastQC (version 0.11.5)