##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568643_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1487102 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.70538604614882 34.0 31.0 34.0 31.0 34.0 2 32.80958333725595 34.0 31.0 34.0 31.0 34.0 3 32.870565704302734 34.0 31.0 34.0 31.0 34.0 4 36.25163304198367 37.0 37.0 37.0 35.0 37.0 5 36.299675476194636 37.0 37.0 37.0 35.0 37.0 6 36.32043733382108 37.0 37.0 37.0 35.0 37.0 7 36.25899904646756 37.0 37.0 37.0 35.0 37.0 8 36.25361004154389 37.0 37.0 37.0 35.0 37.0 9 38.083182592720604 39.0 39.0 39.0 37.0 39.0 10-11 38.011980348355394 39.0 39.0 39.0 35.0 39.0 12-13 37.994637893029534 39.0 39.0 39.0 35.0 39.0 14-15 39.478154491084 41.0 39.0 41.0 36.0 41.0 16-17 39.453123928284676 41.0 39.0 41.0 36.0 41.0 18-19 39.487087973790636 41.0 39.5 41.0 36.5 41.0 20-21 39.44364004621069 41.0 39.0 41.0 36.0 41.0 22-23 39.418847866521595 41.0 39.0 41.0 36.0 41.0 24-25 39.389835734199806 41.0 39.0 41.0 36.0 41.0 26-27 39.29490714154106 41.0 39.0 41.0 36.0 41.0 28-29 39.199962073885985 41.0 39.0 41.0 36.0 41.0 30-31 39.154447711051425 41.0 39.0 41.0 36.0 41.0 32-33 39.02346106723009 40.5 39.0 41.0 35.0 41.0 34-35 38.94123402429692 40.0 38.5 41.0 35.0 41.0 36-37 38.85981829087716 40.0 38.0 41.0 35.0 41.0 38-39 38.72488067395511 40.0 38.0 41.0 35.0 41.0 40-41 38.63931525880538 40.0 38.0 41.0 35.0 41.0 42-43 38.50267231165044 40.0 38.0 41.0 34.0 41.0 44-45 38.39326320588635 40.0 38.0 41.0 34.0 41.0 46-47 38.197063483204246 40.0 38.0 41.0 33.5 41.0 48-49 38.032445656047805 40.0 38.0 41.0 33.0 41.0 50-51 38.029483182727205 40.0 37.5 41.0 33.0 41.0 52-53 38.10217019410908 40.0 37.5 41.0 34.0 41.0 54-55 37.94254630818868 40.0 37.0 41.0 33.0 41.0 56-57 37.76749879967883 40.0 37.0 41.0 33.0 41.0 58-59 37.52717365722055 39.5 36.0 41.0 33.0 41.0 60-61 37.39689510201721 39.0 36.0 41.0 33.0 41.0 62-63 37.16280356021309 39.0 35.0 41.0 32.5 41.0 64-65 36.883698629952754 38.5 35.0 41.0 32.0 41.0 66-67 36.598906799937055 37.5 35.0 40.0 32.0 41.0 68-69 36.265397733309484 37.0 35.0 40.0 32.0 41.0 70-71 35.88374368402437 36.5 35.0 39.0 31.0 41.0 72-73 35.495984808036035 36.0 35.0 39.0 31.0 41.0 74-75 35.14113087064639 35.5 35.0 38.5 31.0 40.0 76-77 33.422210446896045 34.5 32.0 36.0 28.0 39.0 78-79 34.379988393533196 35.0 34.0 37.0 30.0 39.0 80-81 34.30918356642651 35.0 34.0 36.0 31.0 39.0 82-83 34.11814421606587 35.0 34.0 36.0 31.0 37.0 84-85 33.86891988579129 35.0 34.0 36.0 30.0 37.0 86-87 33.692836133634415 35.0 34.0 35.0 30.0 37.0 88-89 33.55127859420537 35.0 34.0 35.0 30.0 36.0 90-91 33.44141155078805 35.0 34.0 35.0 30.0 36.0 92-93 33.35017335730838 35.0 34.0 35.0 30.0 36.0 94-95 33.26315074554402 35.0 34.0 35.0 30.0 36.0 96-97 33.183020734287226 35.0 34.0 35.0 29.0 35.0 98-99 33.10963135010242 35.0 34.0 35.0 29.0 35.0 100-101 31.971572898160314 34.5 32.0 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2781820238163988 1101 2 0.13564892028276176 1101 3 0.12243852945272238 1101 4 0.1373441359000438 1101 5 0.04579476160354545 1101 6 0.08688580149301117 1101 7 0.15618542903506238 1101 8 0.09860845156359943 1101 9 0.08910665240708937 1101 10-11 0.1346064744321751 1101 12-13 0.16306485633785428 1101 14-15 0.15061956492950657 1101 16-17 0.22174480794041074 1101 18-19 0.14698278645659713 1101 20-21 0.20852261550950146 1101 22-23 0.2088296539621055 1101 24-25 0.1623658133531336 1101 26-27 0.054535575018398674 1101 28-29 0.14915748441317334 1101 30-31 0.09534377352868972 1101 32-33 0.0240629917771642 1101 34-35 0.024172431268787875 1101 36-37 -0.021684987044395143 1101 38-39 -0.005903238882723372 1101 40-41 -0.031099362622136084 1101 42-43 -0.10649639649287934 1101 44-45 -0.12241506929638746 1101 46-47 0.11861426948105702 1101 48-49 -0.055782429210729845 1101 50-51 0.012122139626150386 1101 52-53 0.09736433872409833 1101 54-55 -0.04265436623203556 1101 56-57 -0.01142322192261247 1101 58-59 0.07081005145235508 1101 60-61 -0.04246094645449716 1101 62-63 -0.04113556592927381 1101 64-65 -0.18581340309543748 1101 66-67 -0.18255158261368365 1101 68-69 -0.09376093078311953 1101 70-71 -0.17187245387429329 1101 72-73 -0.21274688367703476 1101 74-75 -0.10846962030531415 1101 76-77 -0.27984505985615726 1101 78-79 -0.09396760854556163 1101 80-81 -0.0978646824858771 1101 82-83 -0.15210118529036976 1101 84-85 -0.057842029198972966 1101 86-87 -0.08111246308340014 1101 88-89 -0.042240864967190817 1101 90-91 -0.013700805753657619 1101 92-93 -0.008411563474396644 1101 94-95 -0.12318220303729532 1101 96-97 -0.06183439623631415 1101 98-99 -0.02851798933868821 1101 100-101 -0.2151417868820964 1102 1 0.2103362036929184 1102 2 0.0931240554151671 1102 3 0.1342416937504609 1102 4 0.14694624745443008 1102 5 0.1161207479815971 1102 6 0.11545044003595706 1102 7 0.09958808566982214 1102 8 0.05967717315292731 1102 9 0.17972651699658115 1102 10-11 0.11256940087666578 1102 12-13 0.1634223441140037 1102 14-15 0.17969085517216143 1102 16-17 0.2541970628438577 1102 18-19 0.16680363805619436 1102 20-21 0.17815840271578764 1102 22-23 0.17468046981291963 1102 24-25 0.2156532345364326 1102 26-27 0.2185779679111164 1102 28-29 0.16568307740296717 1102 30-31 0.14869161874204195 1102 32-33 0.16500065136992248 1102 34-35 0.14711864629743587 1102 36-37 -0.14443728897240504 1102 38-39 -0.09976830368054834 1102 40-41 -0.03565720535785033 1102 42-43 -0.0033985904666522515 1102 44-45 0.08398279892361415 1102 46-47 0.0868898839605734 1102 48-49 -0.013226973097786754 1102 50-51 -0.07168219775736162 1102 52-53 0.03748842288288756 1102 54-55 0.006301991943409746 1102 56-57 0.00319661232845192 1102 58-59 0.03733817264425454 1102 60-61 -0.0019639524867898217 1102 62-63 0.13005000916085407 1102 64-65 0.06514478549833314 1102 66-67 0.11149601837534817 1102 68-69 0.07940523782772857 1102 70-71 0.14021336341760104 1102 72-73 0.20246859498747227 1102 74-75 -0.009133526175290285 1102 76-77 -0.05199590250592223 1102 78-79 0.06295132212607513 1102 80-81 0.25479422765676674 1102 82-83 0.1343168074182941 1102 84-85 0.13810526284376579 1102 86-87 0.108961057033504 1102 88-89 0.16269140449870179 1102 90-91 0.053269245164337065 1102 92-93 0.005620716609946896 1102 94-95 0.14518746056013043 1102 96-97 0.04725600721418033 1102 98-99 -0.02802954069405672 1102 100-101 -0.12495500331136355 1103 1 0.15531194866866826 1103 2 0.07918214147325386 1103 3 0.07917333868210363 1103 4 0.05238315289133766 1103 5 0.040766372627217606 1103 6 0.04549517008068449 1103 7 0.08083926692100363 1103 8 0.10237861585437003 1103 9 0.08632262359268594 1103 10-11 0.06020059850786197 1103 12-13 0.1266996073912594 1103 14-15 0.20310797858928709 1103 16-17 0.2138549725017711 1103 18-19 0.08467360592616302 1103 20-21 0.18295900751638783 1103 22-23 0.11146305659550393 1103 24-25 0.06015647903967647 1103 26-27 0.08536433469748772 1103 28-29 0.14018380190369584 1103 30-31 0.05098797103838848 1103 32-33 0.06179394816322059 1103 34-35 -0.04945100027220661 1103 36-37 -0.0067065512416633055 1103 38-39 0.049018945106709566 1103 40-41 -0.05324682294747163 1103 42-43 0.08588194881388489 1103 44-45 0.01170912664994006 1103 46-47 0.0038054008760965985 1103 48-49 0.029578419707604553 1103 50-51 -0.05988228595744971 1103 52-53 0.04862053401500077 1103 54-55 0.051828987470408094 1103 56-57 0.13212939126123047 1103 58-59 0.10423793954402072 1103 60-61 -0.044007044529884354 1103 62-63 -0.07211089300005114 1103 64-65 -0.04112057076702769 1103 66-67 -0.01100760412827384 1103 68-69 -0.019334760912272486 1103 70-71 0.12154644475068466 1103 72-73 0.11853676105562982 1103 74-75 0.02603940899764723 1103 76-77 -0.04957040008041247 1103 78-79 0.036466095640854235 1103 80-81 -0.012830039967504092 1103 82-83 0.032055105156594266 1103 84-85 0.08366690840541224 1103 86-87 0.059534357606807475 1103 88-89 0.011727503534800121 1103 90-91 -0.03777844588335455 1103 92-93 -0.05086198987276447 1103 94-95 0.06193917731184939 1103 96-97 -0.0018462418880673681 1103 98-99 0.05722844456392551 1103 100-101 -0.02396905232541613 1104 1 0.2189985706374955 1104 2 0.028713551127360404 1104 3 0.015414440567376175 1104 4 0.05797250817393973 1104 5 0.06750004353266803 1104 6 0.09437226803753873 1104 7 0.14431472916947286 1104 8 0.15138042559237164 1104 9 0.258960575371745 1104 10-11 0.18762982102911252 1104 12-13 0.19028702680689946 1104 14-15 0.19420313226112995 1104 16-17 0.34066843060356433 1104 18-19 0.11727794221148002 1104 20-21 0.22083099937611195 1104 22-23 0.17781397570715995 1104 24-25 0.2440404574021784 1104 26-27 0.1916046004469507 1104 28-29 0.2372088590514565 1104 30-31 0.1730482096630439 1104 32-33 0.12285241106217626 1104 34-35 0.24684860277585585 1104 36-37 0.0871489843316624 1104 38-39 0.1452581763152594 1104 40-41 0.051012226096858626 1104 42-43 0.052564771324931314 1104 44-45 -0.03825849080234178 1104 46-47 0.15846643283773432 1104 48-49 0.2065529402404067 1104 50-51 0.18672108948027955 1104 52-53 0.06752641488406397 1104 54-55 0.18970778179092207 1104 56-57 0.08210437804603288 1104 58-59 0.1793519870507012 1104 60-61 0.21437379974052817 1104 62-63 0.2149132713247326 1104 64-65 0.17349706716349544 1104 66-67 0.21591681911516503 1104 68-69 0.16108279680589987 1104 70-71 0.2089488542389759 1104 72-73 0.18279450629497518 1104 74-75 0.10920941241305115 1104 76-77 -0.03437752905932712 1104 78-79 0.042707353415849525 1104 80-81 0.17445091725210204 1104 82-83 0.00674853383775087 1104 84-85 0.09953789973652505 1104 86-87 0.06751378583163614 1104 88-89 0.0941782884395721 1104 90-91 0.02492111068122682 1104 92-93 0.03408444783440956 1104 94-95 0.09473341562756588 1104 96-97 0.033249495661657136 1104 98-99 0.11976077396742113 1104 100-101 0.05561973891981964 1105 1 0.31381489061109846 1105 2 0.11716641433013564 1105 3 0.14395952369122256 1105 4 0.1795951272370644 1105 5 0.18441614685024632 1105 6 0.17562370626017554 1105 7 0.19803804335544584 1105 8 0.15732761188616706 1105 9 0.255855642648001 1105 10-11 0.28866957458831166 1105 12-13 0.24092223276038283 1105 14-15 0.3920795015576246 1105 16-17 0.2944146478066685 1105 18-19 0.32774635775687244 1105 20-21 0.4263611553771369 1105 22-23 0.4147744522635861 1105 24-25 0.35115276685125707 1105 26-27 0.37680588648935043 1105 28-29 0.3813499742004396 1105 30-31 0.3350357682231291 1105 32-33 0.38608378797854215 1105 34-35 0.40076881778200146 1105 36-37 0.4272672008053391 1105 38-39 0.489232186275359 1105 40-41 0.4776289511607885 1105 42-43 0.5160626807462094 1105 44-45 0.4971147306861141 1105 46-47 0.4708633149881436 1105 48-49 0.504125817423791 1105 50-51 0.5554157043405397 1105 52-53 0.6792955806932284 1105 54-55 0.5468146639847546 1105 56-57 0.6507835795014074 1105 58-59 0.6755469384007853 1105 60-61 0.6420379850660964 1105 62-63 0.6678356311821432 1105 64-65 0.7123347770354513 1105 66-67 0.5142063653532105 1105 68-69 0.4704718941013937 1105 70-71 0.42208666527498195 1105 72-73 0.4231491882795311 1105 74-75 0.5808780546006247 1105 76-77 0.37405137823881773 1105 78-79 0.4604258221483448 1105 80-81 0.5484392768750652 1105 82-83 0.456926602082234 1105 84-85 0.5218014233711372 1105 86-87 0.5148989885287634 1105 88-89 0.5131824739388193 1105 90-91 0.4566215457300089 1105 92-93 0.520337210307332 1105 94-95 0.4778458054000083 1105 96-97 0.45680959057349213 1105 98-99 0.5489703702230528 1105 100-101 0.4474510142379664 1106 1 0.29933732006100655 1106 2 0.06558649277048545 1106 3 0.14695330832800124 1106 4 0.1046982397606726 1106 5 0.1586215091416534 1106 6 0.10593086493241799 1106 7 0.1351030889802658 1106 8 0.19946288847422977 1106 9 0.189439402590601 1106 10-11 0.25168167077677595 1106 12-13 0.24721132638259036 1106 14-15 0.22976503807288395 1106 16-17 0.2803183845076802 1106 18-19 0.22189205344177765 1106 20-21 0.24539372440860063 1106 22-23 0.19247397554698864 1106 24-25 0.22984754057593193 1106 26-27 0.2559303874335441 1106 28-29 0.25764933505686116 1106 30-31 0.2700691009695575 1106 32-33 0.20312921251161953 1106 34-35 0.24583482300256065 1106 36-37 0.2548329639260487 1106 38-39 0.22469078940329013 1106 40-41 0.15510931906451475 1106 42-43 0.3028392974229277 1106 44-45 0.18841927468006503 1106 46-47 0.2422167961637811 1106 48-49 0.41700316304112306 1106 50-51 0.36234383150017635 1106 52-53 0.42402193084002704 1106 54-55 0.2006735153750654 1106 56-57 0.13507955316094922 1106 58-59 0.19012740138371953 1106 60-61 0.2731485300548542 1106 62-63 0.30039134715941174 1106 64-65 0.2601745311500565 1106 66-67 0.2629958661433349 1106 68-69 0.09148907658745031 1106 70-71 0.17184814190793674 1106 72-73 0.14298574887711624 1106 74-75 0.26077914581063766 1106 76-77 -0.004098395727972104 1106 78-79 0.1679053867207756 1106 80-81 0.20258589694118 1106 82-83 0.10379839701045768 1106 84-85 0.2343560058809615 1106 86-87 0.1883302365754531 1106 88-89 0.28337635189408417 1106 90-91 0.22051229927676985 1106 92-93 0.24366881225997616 1106 94-95 0.256895310415878 1106 96-97 0.2895595916739566 1106 98-99 0.2733347480924877 1106 100-101 0.3399743658681764 1107 1 0.05960895822883572 1107 2 -0.03332577805221604 1107 3 -0.04892101748242794 1107 4 0.08733983697082692 1107 5 0.030287607762488733 1107 6 0.02717860790447446 1107 7 0.07205045111464159 1107 8 0.04360189041097584 1107 9 0.05580897904395954 1107 10-11 0.041847562610968225 1107 12-13 0.07090427089416806 1107 14-15 0.07591263209569377 1107 16-17 0.06222493490681558 1107 18-19 0.009184486577396456 1107 20-21 0.09246703105949905 1107 22-23 0.11948592426749371 1107 24-25 0.02119038744200452 1107 26-27 0.017409740427098086 1107 28-29 -0.06427166570966136 1107 30-31 -0.059311262067858195 1107 32-33 -0.01728918355149034 1107 34-35 -0.101961675589898 1107 36-37 -0.025066341531278624 1107 38-39 4.3662459455617864E-4 1107 40-41 0.05318079225207839 1107 42-43 0.02804881203338283 1107 44-45 0.03269029675391266 1107 46-47 0.06231802956416033 1107 48-49 0.0640075857157214 1107 50-51 -0.007206128018133029 1107 52-53 0.02908818114931222 1107 54-55 0.08111603430131709 1107 56-57 0.23866169954792582 1107 58-59 0.12780469118094118 1107 60-61 0.08151523888713541 1107 62-63 0.10089612211222487 1107 64-65 0.12416617346708847 1107 66-67 0.19795439430740203 1107 68-69 0.04836519275259832 1107 70-71 0.011005404385080908 1107 72-73 0.09486193913519969 1107 74-75 0.08876948049964994 1107 76-77 -0.07806177944898707 1107 78-79 0.09015271073096898 1107 80-81 0.061221069171324416 1107 82-83 0.018735442714124417 1107 84-85 0.14515552252560582 1107 86-87 0.1357678110332401 1107 88-89 0.046049274698681586 1107 90-91 0.02032486660592525 1107 92-93 -0.13632937358575958 1107 94-95 0.0037433296423188267 1107 96-97 0.17322964371413008 1107 98-99 0.14360902217257632 1107 100-101 0.09885552663951458 1108 1 0.17331075224972636 1108 2 0.03587920537918876 1108 3 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-0.5386972840438347 2316 52-53 -0.5057847769901045 2316 54-55 -0.7004159469286932 2316 56-57 -0.6501743567236105 2316 58-59 -0.5631056285387217 2316 60-61 -0.320237706188216 2316 62-63 -0.3744677539630388 2316 64-65 -0.3405160795078359 2316 66-67 -0.32267039599170744 2316 68-69 -0.3740332982929573 2316 70-71 -0.4954365727075256 2316 72-73 -0.5043069420415023 2316 74-75 -0.4447963849638086 2316 76-77 -0.5162555077370143 2316 78-79 -0.5587177690783918 2316 80-81 -0.34614654223859276 2316 82-83 -0.4996295524520278 2316 84-85 -0.5283641178283673 2316 86-87 -0.5981604381555741 2316 88-89 -0.5362234609948331 2316 90-91 -0.5030285225378677 2316 92-93 -0.44771813039310615 2316 94-95 -0.39802814115599716 2316 96-97 -0.5373435009861751 2316 98-99 -0.6228368453218494 2316 100-101 -0.4092801818287306 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 2.0 10 0.0 11 0.0 12 1.0 13 11.0 14 26.0 15 101.0 16 207.0 17 455.0 18 833.0 19 1496.0 20 2240.0 21 3367.0 22 4428.0 23 5110.0 24 5542.0 25 6109.0 26 7456.0 27 9106.0 28 11583.0 29 14863.0 30 18735.0 31 23501.0 32 30221.0 33 40010.0 34 57827.0 35 90115.0 36 172238.0 37 377276.0 38 474606.0 39 129562.0 40 73.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.34397971605163 17.159372919314812 11.533267423952006 24.963379940681556 2 16.070254095518912 18.691049603996053 39.65445163635629 25.58424466412874 3 19.924524343320098 23.247026767498127 28.69897290165705 28.129475987524728 4 12.633094434678993 14.803086809109262 36.98603054800545 35.57778820820629 5 13.807862540699967 37.53306767121556 34.79989940165503 13.859170386429446 6 34.1675817912951 35.668812081931506 16.657913300655594 13.505692826117802 7 29.99819062717974 31.18282535247457 20.45183200679625 18.367152013549447 8 27.449697465271377 35.653102477166996 18.725682569184897 18.171517488376722 9 26.65392151984195 15.0107390078152 18.47102619726152 39.86431327508133 10-11 26.031267525697633 25.222076226109575 27.85790752752669 20.8887487206661 12-13 26.130655462772562 22.338985489899148 28.180582098605207 23.349776948723086 14-15 22.897958579841866 24.223254356459744 24.89503073763602 27.98375632606237 16-17 23.418400351825227 27.595013657435736 25.544111970799584 23.442474019939453 18-19 23.3830295433669 26.76363154645747 27.075244334282385 22.77809457589325 20-21 23.887298920988606 25.785655590537836 27.188518339696945 23.138527148776614 22-23 23.62874234585119 25.630252665923386 27.04595918773561 23.69504580048981 24-25 23.260240387007748 25.962005296206986 27.096056625571073 23.681697691214186 26-27 23.37186689278879 26.134858267960098 26.933424876034056 23.55984996321705 28-29 23.3739475200779 26.128702970849645 27.025593057011616 23.471756452060845 30-31 23.444558611312473 25.963013969451993 27.026021079925922 23.56640633930961 32-33 23.21737177409485 26.109641436834863 27.054432042993685 23.618554746076597 34-35 23.48053462371781 26.154998110418788 27.111993662842227 23.25247360302118 36-37 23.56426242593394 25.97388275111013 27.050339200115125 23.41151562284081 38-39 23.454544476438066 26.101605673316293 26.995592770368138 23.448257079877507 40-41 23.476869777594274 26.090846492036192 26.973469203860933 23.458814526508608 42-43 23.45619197607158 25.88070623265923 27.000602514151684 23.662499277117508 44-45 23.322441903783332 26.27620701202742 26.928650489341017 23.472700594848234 46-47 23.55588251512001 26.183913410109056 26.78498179681017 23.475222277960757 48-49 23.469977177086708 26.29096726384606 26.649147133148904 23.58990842591833 50-51 23.496269926339956 26.397012444338046 26.576993373689227 23.529724255632768 52-53 23.546669966148926 26.27240767613788 26.651231724521924 23.529690633191265 54-55 23.38864449109745 26.307610372388712 26.71514798581402 23.588597150699815 56-57 23.50396946544353 26.47094819319724 26.479959007519323 23.54512333383991 58-59 23.54703981300543 26.258454362915256 26.619693874394628 23.574811949684687 60-61 23.580569315544366 26.262464263535474 26.790370395026837 23.366596025893323 62-63 23.375261878288082 26.403854229744127 26.71725079828538 23.503633093682406 64-65 23.397382015173175 26.307748379730185 26.675242653812997 23.61962695128364 66-67 23.333806289010436 26.399937596748575 26.640842390098324 23.62541372414266 68-69 23.515905432176137 26.29722103796512 26.665420394835053 23.52145313502369 70-71 23.38857724621445 26.163033873937362 26.71955252565056 23.72883635419763 72-73 23.296350889179088 26.33548337639247 26.687543961342264 23.680621773086177 74-75 23.355963477959143 26.421590449074777 26.636135248288284 23.5863108246778 76-77 23.32846032081189 26.315478023699786 26.60355510247448 23.75250655301385 78-79 23.344027511226535 26.322740471063856 26.557929449358554 23.775302568351062 80-81 23.450207181484526 26.51711180537717 26.36275117644923 23.66992983668908 82-83 23.400076121207558 26.464223704897176 26.50003160509501 23.63566856880026 84-85 23.41006198633315 26.506957828044076 26.39644086283254 23.68653932279023 86-87 23.31296037528024 26.489541403346912 26.50211619646803 23.695382024904816 88-89 23.31272501818974 26.48056421146633 26.408679431538655 23.798031338805274 90-91 23.36295694579121 26.44213376083147 26.428651161789844 23.766258131587474 92-93 23.37358163730531 26.358548371261687 26.548212563764963 23.719657427668043 94-95 23.25368401091519 26.228463145096974 26.680348758861193 23.837504085126643 96-97 23.28898757449052 26.08536603407164 26.703615488379413 23.92203090305843 98-99 23.31224976430605 26.16629838786684 26.46076452258022 24.060687325246892 100-101 23.374295980765073 26.262830037122924 26.502795903264538 23.86007807884746 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1.0 1 2.5 2 18.5 3 29.0 4 17.0 5 18.0 6 43.0 7 85.0 8 166.5 9 224.5 10 210.0 11 208.0 12 233.0 13 268.0 14 311.0 15 356.5 16 418.5 17 465.0 18 491.0 19 526.0 20 588.0 21 653.0 22 769.5 23 954.0 24 1136.5 25 1460.5 26 2086.5 27 3082.0 28 4718.5 29 7313.5 30 10863.5 31 15291.0 32 19822.5 33 23447.0 34 26871.0 35 29551.5 36 32983.5 37 35771.5 38 36345.0 39 37520.5 40 38321.0 41 39337.0 42 41227.0 43 44028.0 44 47494.0 45 51217.0 46 54855.5 47 57739.0 48 59961.5 49 62105.5 50 63555.0 51 64249.0 52 64316.5 53 64516.0 54 64277.0 55 60906.5 56 56118.5 57 51305.5 58 46152.0 59 39434.0 60 31863.0 61 25408.5 62 19518.0 63 13909.0 64 9206.5 65 5924.5 66 3950.5 67 2733.5 68 1991.0 69 1505.5 70 1152.0 71 852.0 72 643.0 73 530.0 74 418.5 75 347.5 76 255.0 77 155.0 78 98.0 79 62.0 80 49.0 81 35.5 82 21.0 83 14.5 84 8.0 85 6.0 86 4.5 87 2.5 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01425591519613315 2 0.11007987347202815 3 0.0 4 0.0 5 0.0 6 0.003698468565034544 7 0.026830708317250603 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0012776527770119333 30-31 0.0 32-33 0.0 34-35 0.0 36-37 3.3622441500314034E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 3.3622441500314034E-5 62-63 5.715815055053386E-4 64-65 2.689795320025123E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 2.689795320025123E-4 100-101 0.001042295686509735 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1487102.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.15409810811962 #Duplication Level Percentage of deduplicated Percentage of total 1 66.51264159569523 34.023941936163915 2 17.290491758589877 17.689590235130805 3 6.6709256350170625 10.23735553216899 4 3.2215964629607163 6.591914461242545 5 1.8365182935961202 4.697271848398618 6 1.1508325938537642 3.532188204721033 7 0.7436350195766854 2.6627985123641453 8 0.5210060802462853 2.132127691507625 9 0.36686888745914176 1.689016235571147 >10 1.6228918793759577 13.67405115063277 >50 0.04857935633306615 1.632677103247853 >100 0.013219324584801028 1.099178564972811 >500 5.287418075328456E-4 0.15302015800188123 >1k 2.643709037664228E-4 0.18486836587589187 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 6.724488300062807E-5 0.0 0.0 0.0 0.0 10-11 6.724488300062807E-5 0.0 0.0 0.0 0.0 12-13 6.724488300062807E-5 0.0 0.0 0.0 0.0 14-15 6.724488300062807E-5 0.0 0.0 0.0 3.3622441500314034E-5 16-17 6.724488300062807E-5 0.0 0.0 3.3622441500314034E-5 6.724488300062807E-5 18-19 6.724488300062807E-5 0.0 0.0 6.724488300062807E-5 6.724488300062807E-5 20-21 6.724488300062807E-5 0.0 0.0 6.724488300062807E-5 6.724488300062807E-5 22-23 6.724488300062807E-5 0.0 0.0 3.026019735028263E-4 1.6811220750157017E-4 24-25 6.724488300062807E-5 0.0 0.0 6.724488300062807E-4 2.689795320025123E-4 26-27 6.724488300062807E-5 0.0 0.0 0.001075918128010049 3.3622441500314034E-4 28-29 6.724488300062807E-5 0.0 0.0 0.0013112752185122473 3.3622441500314034E-4 30-31 6.724488300062807E-5 0.0 0.0 0.0016811220750157017 3.3622441500314034E-4 32-33 6.724488300062807E-5 0.0 0.0 0.0026225504370244947 3.3622441500314034E-4 34-35 6.724488300062807E-5 0.0 0.0 0.004101937863038313 4.707141810043965E-4 36-37 6.724488300062807E-5 0.0 0.0 0.007027090273565633 4.707141810043965E-4 38-39 6.724488300062807E-5 0.0 0.0 0.012204946264613995 4.707141810043965E-4 40-41 6.724488300062807E-5 0.0 0.0 0.02000535269268685 4.707141810043965E-4 42-43 6.724488300062807E-5 0.0 0.0 0.028545452833766614 4.707141810043965E-4 44-45 6.724488300062807E-5 0.0 0.0 0.039237389230866476 4.707141810043965E-4 46-47 6.724488300062807E-5 0.0 0.0 0.05157682526148173 5.043366225047105E-4 48-49 6.724488300062807E-5 0.0 0.0 0.06566462825011331 6.724488300062807E-4 50-51 6.724488300062807E-5 0.0 0.0 0.08076110448375431 6.724488300062807E-4 52-53 6.724488300062807E-5 0.0 0.0 0.09683263152090442 7.060712715065947E-4 54-55 6.724488300062807E-5 0.0 0.0 0.11599742317608341 7.396937130069088E-4 56-57 6.724488300062807E-5 0.0 0.0 0.13765027550228565 7.396937130069088E-4 58-59 6.724488300062807E-5 0.0 0.0 0.1638757798725306 7.396937130069088E-4 60-61 6.724488300062807E-5 0.0 0.0 0.1962205685958327 7.396937130069088E-4 62-63 6.724488300062807E-5 0.0 0.0 0.23223020344266904 7.396937130069088E-4 64-65 6.724488300062807E-5 0.0 0.0 0.26958473594951793 7.396937130069088E-4 66-67 6.724488300062807E-5 0.0 0.0 0.31524401150694437 7.733161545072228E-4 68-69 6.724488300062807E-5 0.0 0.0 0.37166246834447136 8.069385960075368E-4 70-71 6.724488300062807E-5 0.0 0.0 0.4365874028815777 8.069385960075368E-4 72-73 6.724488300062807E-5 0.0 0.0 0.5085394276922498 8.069385960075368E-4 74-75 6.724488300062807E-5 0.0 0.0 0.5861064002334743 8.069385960075368E-4 76-77 6.724488300062807E-5 0.0 0.0 0.6732221461607879 8.069385960075368E-4 78-79 6.724488300062807E-5 0.0 0.0 0.7757369702952455 8.069385960075368E-4 80-81 6.724488300062807E-5 0.0 0.0 0.8893808225663068 8.741834790081649E-4 82-83 6.724488300062807E-5 0.0 0.0 1.015397733309484 0.001008673245009421 84-85 6.724488300062807E-5 0.0 0.0 1.1529135190457682 0.001008673245009421 86-87 6.724488300062807E-5 0.0 0.0 1.3043153731216823 0.001042295686509735 88-89 6.724488300062807E-5 0.0 0.0 1.4789503342743133 0.001075918128010049 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1370 0.0 47.220974 1 GTATCAA 2250 0.0 41.86 1 TCAACGC 2475 0.0 37.240627 4 CAACGCA 2610 0.0 35.31439 5 ATCAACG 2685 0.0 34.504898 3 GTACATG 4695 0.0 32.623974 1 TATCAAC 2835 0.0 32.54559 2 AACGCAG 2850 0.0 32.507248 6 TACATGG 4855 0.0 30.661867 2 ACATGGG 4705 0.0 30.091726 3 TATAGCG 70 0.008340677 27.177244 2 ACGCAGA 3550 0.0 26.096489 7 CGCAGAG 3555 0.0 25.920044 8 GAGTACA 3230 0.0 24.446808 1 CATGGGG 3225 0.0 23.718468 4 AGTACAT 3155 0.0 22.611813 2 GCAGAGT 4415 0.0 20.763485 9 GGACCGT 240 1.4105717E-7 19.796082 6 GTACTAT 485 0.0 18.63496 1 GAGTACT 2260 0.0 18.59981 12-13 >>END_MODULE