FastQCFastQC Report
Fri 10 Feb 2017
SRR2568641_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568641_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880100
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACG554.961659E-543.1828544
GTACATG25000.034.960841
GTATCAA7850.034.4912721
TACATGG25000.033.820812
GGTATCA6050.030.6205671
ACATGGG26400.030.587853
TCAACGC8750.030.4007264
CAACGCA8900.029.8883535
ATCAACG8800.029.6882113
AACGCAG9300.028.6028376
TACACCG1658.403549E-825.909715
TATCAAC10700.024.8604092
CATGGGG19850.024.1693444
GTATTAG1601.8583451E-623.7505681
GTATAAA3100.022.984421
TTACACC3700.021.8248464
AGTACAT16150.020.8828532
TATGCCG1404.8497136E-420.357635
ACGCAGA13100.020.305831
GTATTAC2351.1308293E-720.213251
Produced by FastQC (version 0.11.5)