##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568641_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 880100 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13005113055335 34.0 31.0 34.0 30.0 34.0 2 32.25591410067038 34.0 31.0 34.0 30.0 34.0 3 32.317829792069084 34.0 31.0 34.0 30.0 34.0 4 35.64350641972503 37.0 35.0 37.0 33.0 37.0 5 35.67476423133735 37.0 35.0 37.0 35.0 37.0 6 35.79253834791501 37.0 36.0 37.0 35.0 37.0 7 35.78104192705374 37.0 36.0 37.0 35.0 37.0 8 35.801061243040564 37.0 36.0 37.0 35.0 37.0 9 37.6124531303261 39.0 38.0 39.0 35.0 39.0 10-11 37.589847744574485 39.0 38.0 39.0 35.0 39.0 12-13 37.552205431201 39.0 38.0 39.0 35.0 39.0 14-15 38.98974093852971 41.0 39.0 41.0 36.0 41.0 16-17 38.94808601295307 41.0 39.0 41.0 35.5 41.0 18-19 38.90008919440972 41.0 39.0 41.0 35.0 41.0 20-21 38.88165492557664 41.0 39.0 41.0 35.0 41.0 22-23 38.81533121236223 41.0 39.0 41.0 35.0 41.0 24-25 38.74605499375071 41.0 39.0 41.0 35.0 41.0 26-27 38.64788489944324 40.0 38.5 41.0 34.0 41.0 28-29 38.51849789796614 40.0 38.0 41.0 34.0 41.0 30-31 38.43851153278037 40.0 38.0 41.0 34.0 41.0 32-33 38.336584479036475 40.0 38.0 41.0 34.0 41.0 34-35 38.186926485626635 40.0 38.0 41.0 33.0 41.0 36-37 38.07941881604363 40.0 38.0 41.0 33.0 41.0 38-39 37.9732842858766 40.0 38.0 41.0 33.0 41.0 40-41 37.82714350641973 40.0 38.0 41.0 32.5 41.0 42-43 37.65784626746961 40.0 37.0 41.0 32.0 41.0 44-45 37.46003692762186 40.0 37.0 41.0 31.0 41.0 46-47 37.53861379388706 40.0 37.0 41.0 31.5 41.0 48-49 37.771770253380296 40.0 37.0 41.0 32.0 41.0 50-51 36.61323542779229 38.5 35.5 40.0 30.5 40.5 52-53 36.74276332234973 39.0 36.0 40.0 31.0 40.5 54-55 37.31527610498807 40.0 36.0 41.0 32.0 41.0 56-57 37.24050278377457 39.5 36.0 41.0 31.0 41.0 58-59 37.021782183842745 39.0 35.5 41.0 31.0 41.0 60-61 36.751954323372345 39.0 35.0 41.0 31.0 41.0 62-63 36.42629246676514 38.5 35.0 40.5 30.5 41.0 64-65 36.183585388024085 38.0 35.0 40.0 30.5 41.0 66-67 36.00038234291558 37.0 35.0 40.0 30.0 41.0 68-69 35.646658902397455 37.0 35.0 39.0 30.0 41.0 70-71 35.24429155777752 36.0 35.0 39.0 30.0 41.0 72-73 34.86099534143847 35.5 34.0 38.5 29.0 40.5 74-75 34.498341665719806 35.0 34.0 37.0 29.0 39.0 76-77 34.15453584819907 35.0 34.0 37.0 29.0 39.0 78-79 33.83504033632542 35.0 34.0 36.5 29.0 39.0 80-81 33.529329621633906 35.0 34.0 36.0 29.0 37.0 82-83 33.27728439950006 35.0 34.0 35.5 28.5 37.0 84-85 33.05372287240086 35.0 34.0 35.0 28.0 36.5 86-87 32.87273093966594 35.0 33.0 35.0 27.0 36.0 88-89 32.728410407908186 35.0 33.0 35.0 27.0 36.0 90-91 32.604019429610275 35.0 33.0 35.0 27.0 36.0 92-93 32.49479490966935 35.0 33.0 35.0 26.5 35.0 94-95 32.38652709919327 35.0 33.0 35.0 26.0 35.0 96-97 32.30553403022384 35.0 33.0 35.0 25.5 35.0 98-99 32.18888705828883 35.0 33.0 35.0 25.0 35.0 100-101 30.734654584706284 34.0 30.0 35.0 21.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22117325482435746 1101 2 0.25009408602642935 1101 3 0.3302553870778482 1101 4 0.29594769968473145 1101 5 0.16214224398400034 1101 6 0.22633078119531547 1101 7 0.35537784302661635 1101 8 0.3326447603661933 1101 9 0.3216919117466972 1101 10-11 0.4218874098447145 1101 12-13 0.3689996154049169 1101 14-15 0.5036911030749422 1101 16-17 0.5811893610214298 1101 18-19 0.5647898534909501 1101 20-21 0.35963712459932395 1101 22-23 0.24267569970199077 1101 24-25 0.4095715596800815 1101 26-27 0.37490482509504375 1101 28-29 0.556840357679043 1101 30-31 0.5897652459439442 1101 32-33 0.3626828082451752 1101 34-35 0.08758474110052106 1101 36-37 0.3143101159098052 1101 38-39 0.3153925055314559 1101 40-41 0.2176346804948892 1101 42-43 0.10717312839266668 1101 44-45 0.41073782550920157 1101 46-47 0.19487368748733047 1101 48-49 0.3914467595767661 1101 50-51 0.14093449180138862 1101 52-53 0.21957733813522395 1101 54-55 0.3020427411784965 1101 56-57 0.3535325055509233 1101 58-59 0.2161709226365076 1101 60-61 0.42914800040819046 1101 62-63 0.4447967704483702 1101 64-65 0.4092411518270396 1101 66-67 0.45860553989459873 1101 68-69 0.5246954851907333 1101 70-71 0.6150564586473664 1101 72-73 0.44829328763620424 1101 74-75 0.4651033626208232 1101 76-77 0.359722669604011 1101 78-79 0.3924701714977701 1101 80-81 0.4924979434254979 1101 82-83 0.29425980973875454 1101 84-85 0.431354670792615 1101 86-87 0.46686453245730064 1101 88-89 0.3363247581176836 1101 90-91 0.24331245179880057 1101 92-93 0.3829868697216128 1101 94-95 0.2715211455597242 1101 96-97 0.12057549605001583 1101 98-99 0.25958502814119555 1101 100-101 -0.20387429853719397 1102 1 0.4054248262663336 1102 2 0.3647448506384947 1102 3 0.5303738315848321 1102 4 0.546416786841128 1102 5 0.49040357243882937 1102 6 0.4357430149883541 1102 7 0.4421237854227158 1102 8 0.5245365888679316 1102 9 0.5148620027496023 1102 10-11 0.5090408717079313 1102 12-13 0.4182654940051549 1102 14-15 0.5277523424032111 1102 16-17 0.4924556618001148 1102 18-19 0.5495855660343452 1102 20-21 0.538385717337853 1102 22-23 0.5757363761256755 1102 24-25 0.5571405154920654 1102 26-27 0.6528272380793751 1102 28-29 0.6677765383714132 1102 30-31 0.6737347787331345 1102 32-33 0.6459410876334886 1102 34-35 0.6356984243728689 1102 36-37 0.5672184562794484 1102 38-39 0.5593999171085358 1102 40-41 0.5938631024856988 1102 42-43 0.5867561099106311 1102 44-45 0.39217663308965456 1102 46-47 0.4312144558520927 1102 48-49 0.6661031532347081 1102 50-51 0.3362359975711584 1102 52-53 0.4586006986756104 1102 54-55 0.43308810190652736 1102 56-57 0.5595028123339247 1102 58-59 0.42485313954241377 1102 60-61 0.5082126406183463 1102 62-63 0.5367056033595148 1102 64-65 0.3699844204263414 1102 66-67 0.42182910683333574 1102 68-69 0.40813025862163954 1102 70-71 0.4851369070946916 1102 72-73 0.41307950116467396 1102 74-75 0.4605502854090773 1102 76-77 0.2112741753737808 1102 78-79 0.3810596264184838 1102 80-81 0.3274466956536557 1102 82-83 0.37703023848441575 1102 84-85 0.44420179528834325 1102 86-87 0.30625495369756806 1102 88-89 0.41143733903521706 1102 90-91 0.2462149285789934 1102 92-93 0.2597049014319026 1102 94-95 0.33956516938935266 1102 96-97 0.18947560394006047 1102 98-99 0.1694939079610691 1102 100-101 -0.10916266986658485 1103 1 0.3431343398718738 1103 2 0.29617702840583604 1103 3 0.4145315208442355 1103 4 0.29837762871156315 1103 5 0.31414062002153287 1103 6 0.2877376138670371 1103 7 0.44025394070495594 1103 8 0.34881521627861645 1103 9 0.4185943537050747 1103 10-11 0.4305462875062105 1103 12-13 0.4714115004043151 1103 14-15 0.38414415853025474 1103 16-17 0.4003415924661482 1103 18-19 0.47096842921571636 1103 20-21 0.5434448654870891 1103 22-23 0.5090905018191592 1103 24-25 0.6215121948298403 1103 26-27 0.7087794792511843 1103 28-29 0.2968868210496609 1103 30-31 0.26349539751377193 1103 32-33 0.35011815048022754 1103 34-35 0.2763440638480219 1103 36-37 0.3897130991890094 1103 38-39 0.39373666528125284 1103 40-41 0.3214367074406397 1103 42-43 0.38610371903383367 1103 44-45 0.28610697623697945 1103 46-47 0.3210036947049417 1103 48-49 0.3585870819633925 1103 50-51 0.26466013236436225 1103 52-53 0.21290104798725906 1103 54-55 0.26424802206386033 1103 56-57 0.41263120638095074 1103 58-59 0.38402623960992344 1103 60-61 0.29325241518024114 1103 62-63 0.28213813337389837 1103 64-65 0.36892327621053056 1103 66-67 0.30463284652277167 1103 68-69 0.3077904869951382 1103 70-71 0.39530877429755407 1103 72-73 0.29905639962946395 1103 74-75 0.07861909099806041 1103 76-77 0.25610935311176775 1103 78-79 0.3498652154048969 1103 80-81 0.2570060933112117 1103 82-83 0.3066710932705661 1103 84-85 0.30502091643197815 1103 86-87 0.2470957967421512 1103 88-89 0.26590794108412297 1103 90-91 0.2756789259958481 1103 92-93 0.290843539997077 1103 94-95 0.08217238458293963 1103 96-97 0.0506000058539442 1103 98-99 0.09392957884491437 1103 100-101 0.15445828296166297 1104 1 0.12242811160577816 1104 2 0.1337402864420838 1104 3 0.21663493889857222 1104 4 0.2533435612551713 1104 5 0.3099634692483022 1104 6 0.05879441180885436 1104 7 0.06039049324800061 1104 8 0.1272254958862078 1104 9 0.25173166958531823 1104 10-11 0.16904958116485602 1104 12-13 0.20112440080293936 1104 14-15 0.22477348764167004 1104 16-17 0.13801935108197227 1104 18-19 0.21243036130698556 1104 20-21 0.030982678950095988 1104 22-23 0.03627096038691491 1104 24-25 0.24823041031711313 1104 26-27 0.16049486756364217 1104 28-29 0.14477916223312093 1104 30-31 0.08609724648609784 1104 32-33 0.22960699727562428 1104 34-35 0.060372646585292955 1104 36-37 -0.0037525930447372957 1104 38-39 -0.09558156151471309 1104 40-41 -0.06304493005900014 1104 42-43 -0.14694948839598254 1104 44-45 -0.03240112361883973 1104 46-47 0.060208127715029036 1104 48-49 -0.06236299012957147 1104 50-51 -0.009978696054396607 1104 52-53 0.12043122807829576 1104 54-55 0.12317199933337264 1104 56-57 0.12415123691443597 1104 58-59 0.1468064833123961 1104 60-61 -0.19728644433744336 1104 62-63 -0.16871355303586455 1104 64-65 -0.23399436818741748 1104 66-67 -0.11758945423208189 1104 68-69 -0.12745448770164103 1104 70-71 0.011838558584116754 1104 72-73 0.06080401773469646 1104 74-75 0.06902436650624821 1104 76-77 0.11621155548094464 1104 78-79 -0.033684161203055396 1104 80-81 -0.05341840698563516 1104 82-83 -0.15964594611197924 1104 84-85 -0.005253036806557532 1104 86-87 -0.10076332513735053 1104 88-89 -0.007840327271310343 1104 90-91 -0.1497649404484207 1104 92-93 -0.00349433566376689 1104 94-95 0.06253553518654087 1104 96-97 -0.06310967156542091 1104 98-99 -0.16267540829144878 1104 100-101 -0.41255494821641747 1105 1 0.1430989148521462 1105 2 0.1662493276506396 1105 3 0.37486516012091897 1105 4 0.19744530660151582 1105 5 0.20866262378947198 1105 6 0.2899983742167791 1105 7 0.25616138478977746 1105 8 0.199654950268922 1105 9 0.21496811986774134 1105 10-11 0.37802547530711905 1105 12-13 0.3462267267218806 1105 14-15 0.3701464450542531 1105 16-17 0.327763778189869 1105 18-19 0.4413241286183265 1105 20-21 0.3279818443769926 1105 22-23 0.31671171174460255 1105 24-25 0.382022154091068 1105 26-27 0.3451456773414492 1105 28-29 0.4708099809614268 1105 30-31 0.2621589081332232 1105 32-33 0.31219647197838185 1105 34-35 0.407529352803472 1105 36-37 0.18305722105107236 1105 38-39 0.24442986850814918 1105 40-41 0.46019006879194535 1105 42-43 0.33582837001687693 1105 44-45 0.4986595099310023 1105 46-47 0.6397314534283538 1105 48-49 0.5323202349511718 1105 50-51 0.2973195852088324 1105 52-53 0.46062257512765825 1105 54-55 0.48662858338939685 1105 56-57 0.6421595705501488 1105 58-59 0.6555574055761468 1105 60-61 0.4616090163106108 1105 62-63 0.45318703600340626 1105 64-65 0.5491496742911437 1105 66-67 0.5775842337343349 1105 68-69 0.5017440802192965 1105 70-71 0.46401237959842234 1105 72-73 0.5291129186382051 1105 74-75 0.5140891971120993 1105 76-77 0.47583545330017074 1105 78-79 0.4140658176302381 1105 80-81 0.35470749259949486 1105 82-83 0.4290637313073802 1105 84-85 0.36186841172205675 1105 86-87 0.5075179985681544 1105 88-89 0.3677629239351887 1105 90-91 0.1362895245176503 1105 92-93 0.1943675243456653 1105 94-95 0.19443588232057607 1105 96-97 0.3474503995547238 1105 98-99 0.3691699053991826 1105 100-101 0.09168595312252847 1106 1 0.3827940419292375 1106 2 0.28561585701240944 1106 3 0.4032134849077238 1106 4 0.3582601691565088 1106 5 0.2775345752937497 1106 6 0.31973842895192917 1106 7 0.34861802166035005 1106 8 0.275905872640287 1106 9 0.4921091889778708 1106 10-11 0.3886269081351301 1106 12-13 0.3232421665178862 1106 14-15 0.3526003731886789 1106 16-17 0.46618953546843045 1106 18-19 0.4290133898806019 1106 20-21 0.5420934256250689 1106 22-23 0.5677555092058668 1106 24-25 0.5806822637481801 1106 26-27 0.39458244703349976 1106 28-29 0.4859952491730084 1106 30-31 0.3377700083471211 1106 32-33 0.4777306067239948 1106 34-35 0.475308469381865 1106 36-37 0.4433223969788145 1106 38-39 0.489220221671296 1106 40-41 0.4143563851910059 1106 42-43 0.4531189800429374 1106 44-45 0.48250750313114565 1106 46-47 0.3639237363324952 1106 48-49 0.4217874545736251 1106 50-51 0.37986364887347435 1106 52-53 0.44723067705085384 1106 54-55 0.3912156879372688 1106 56-57 0.4348291701242246 1106 58-59 0.3452856913197877 1106 60-61 0.36495150330401316 1106 62-63 0.3330569232639462 1106 64-65 0.36202270928988156 1106 66-67 0.406018490840097 1106 68-69 0.40250626252657895 1106 70-71 0.3511748383836064 1106 72-73 0.3094530985356485 1106 74-75 0.24931023932168017 1106 76-77 0.44988996427988326 1106 78-79 0.12084746547585468 1106 80-81 0.22381248881660554 1106 82-83 0.2526105877723879 1106 84-85 0.10154952809497075 1106 86-87 0.13354428264918994 1106 88-89 0.18747785280751827 1106 90-91 0.0858696704157893 1106 92-93 0.04504377433398332 1106 94-95 0.12456917081965457 1106 96-97 0.1873734340864459 1106 98-99 0.12642745016338353 1106 100-101 0.1993185177352217 1107 1 0.15335180439273444 1107 2 0.08000950213905611 1107 3 0.21678871407925726 1107 4 0.09919386359701576 1107 5 0.16828816306207273 1107 6 0.04305601811462623 1107 7 0.16090613615226346 1107 8 0.2850471960444452 1107 9 0.1040058060880682 1107 10-11 0.08180200808444482 1107 12-13 0.18802808793493853 1107 14-15 0.2110924570503343 1107 16-17 0.2196987744746508 1107 18-19 0.06907840960231226 1107 20-21 -0.040011849629763674 1107 22-23 -0.027476292741916097 1107 24-25 0.14297005901522652 1107 26-27 0.034553614643158426 1107 28-29 0.20853137585164916 1107 30-31 0.16976706637071004 1107 32-33 -0.059123266175980405 1107 34-35 -0.05912120984821456 1107 36-37 -0.13858304350681294 1107 38-39 -0.12013536546623982 1107 40-41 -0.08822933626358775 1107 42-43 -0.0856285347914465 1107 44-45 0.18670990702210588 1107 46-47 -0.0456462422283721 1107 48-49 -0.08894935473547605 1107 50-51 -0.027001856580803008 1107 52-53 9.045084005308013E-4 1107 54-55 -0.035196594814244975 1107 56-57 0.12942609764868962 1107 58-59 -0.005718653969154275 1107 60-61 0.08717159736043101 1107 62-63 0.15475067935315678 1107 64-65 0.1536703820767329 1107 66-67 0.14118379186276542 1107 68-69 0.29311939135169496 1107 70-71 0.2890747473406705 1107 72-73 0.38582956425664605 1107 74-75 0.4126480290342158 1107 76-77 0.32825698860610686 1107 78-79 0.15479894584927223 1107 80-81 0.10188646471129204 1107 82-83 0.20328332226539914 1107 84-85 0.14793928633958586 1107 86-87 0.2823669174801964 1107 88-89 0.11784980085813856 1107 90-91 0.1396628918671894 1107 92-93 0.08690500855949068 1107 94-95 0.17256973776301976 1107 96-97 0.18504564632756626 1107 98-99 0.26931441774418374 1107 100-101 0.17487802316651724 1108 1 0.1035906954848329 1108 2 -0.025770207059224504 1108 3 0.11373061927599082 1108 4 0.1205091031935055 1108 5 0.049203647492774394 1108 6 -0.09165167494503379 1108 7 0.016239556132916277 1108 8 0.13927530732181737 1108 9 0.13773938336692026 1108 10-11 0.008864964024283495 1108 12-13 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-0.4728238894125525 2316 84-85 -0.6272676587362085 2316 86-87 -0.3706939984177424 2316 88-89 -0.28055408297098694 2316 90-91 -0.2626686719939926 2316 92-93 -0.19798693771181064 2316 94-95 -0.08307535722049408 2316 96-97 0.055779988294681004 2316 98-99 -0.03280412901420959 2316 100-101 0.29501910210665017 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 11.0 7 261.0 8 431.0 9 296.0 10 289.0 11 338.0 12 523.0 13 711.0 14 946.0 15 1150.0 16 1412.0 17 1674.0 18 2030.0 19 2333.0 20 2863.0 21 3203.0 22 3841.0 23 4325.0 24 4737.0 25 5269.0 26 6132.0 27 7225.0 28 8478.0 29 10197.0 30 12554.0 31 15472.0 32 19902.0 33 26641.0 34 39393.0 35 62075.0 36 115302.0 37 227117.0 38 250180.0 39 42769.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.924054919170004 15.5424023671194 10.51454621689277 26.01899649681782 2 16.813856611389003 18.366737042095718 38.1949952388636 26.62441110765168 3 21.36169545525819 21.68302476780889 26.743393319349977 30.211886457582942 4 13.254986086792508 14.200675151318205 35.40385797506872 37.140480786820575 5 15.177263704260897 36.472428991685916 33.53346824638411 14.816839057669078 6 36.29264922612967 33.50612517143776 16.45722947490901 13.743996127523559 7 32.08969893927084 29.507246706171774 19.102328831720744 19.30072552283664 8 28.30860588072872 34.204560820215875 18.53439704747739 18.952436251578014 9 27.43140897805201 14.610214366474061 17.23988250467311 40.71849415080082 10-11 26.876051088586884 24.59331394027544 26.963319849097765 21.567315122039908 12-13 27.401575787325527 21.32698836908057 26.884229400455418 24.387206443138485 14-15 24.035507328712647 23.214634700602204 23.890410180661288 28.85944779002386 16-17 25.024826724235883 26.044824451766846 24.416543574593796 24.51380524940348 18-19 24.806087045938607 25.754446803397574 25.422040828384553 24.01742532227927 20-21 25.439885738504113 24.96515967464562 25.392219969287112 24.20273461756316 22-23 24.96496118887098 24.960813911672087 25.09824217939985 24.975982720057086 24-25 24.73998324030281 25.034669848168505 25.39446361867428 24.830883292854402 26-27 24.883957698675214 25.301425914163357 25.143540207734183 24.67107617942725 28-29 24.911470563003473 25.308652847003295 25.18957201918701 24.59030457080622 30-31 25.097754904222352 25.20741312285016 25.181220149385176 24.513611823542313 32-33 24.709193490933654 25.30787852000152 25.142044244062152 24.840883745002674 34-35 24.8825133507556 25.31081695261902 25.32280422679241 24.483865469832974 36-37 25.046003373486037 25.216666089079805 25.263592654685812 24.47373788274835 38-39 24.984277310184382 25.34151045764816 25.2177173551794 24.45649487698806 40-41 25.078456993523464 25.34501761163504 25.067435518691056 24.509089876150437 42-43 24.86946048591207 25.0920494604291 25.275097361371895 24.763392692286935 44-45 24.880623886130667 25.28046640340008 25.13167590144944 24.707233809019808 46-47 24.799946368298663 25.292346744856843 25.13054262896464 24.777164257879853 48-49 24.79302735697696 25.205665925814824 25.325372584845717 24.6759341323625 50-51 24.853932073585057 25.478200597679727 25.15879418682605 24.509073141909166 52-53 24.852800818088856 25.405010794227927 25.155891375979998 24.586297011703216 54-55 24.723554141574823 25.219861379388707 25.326156118622883 24.73042836041359 56-57 24.79343256448131 25.349278491080558 25.26036813998409 24.59692080445404 58-59 24.814396091353256 25.349562549710257 25.282127031019204 24.55391432791728 60-61 24.999091012384955 25.298772866719695 25.221054425633447 24.481081695261903 62-63 24.68609690957216 25.389946579236067 25.27910545359927 24.644851057592504 64-65 24.697199274380438 25.271468186536268 25.33953052891485 24.691802010168445 66-67 24.625641637478733 25.40188450383349 25.286212215470883 24.686261643216895 68-69 24.64637649540017 25.357657448820042 25.24368432057016 24.75228173520963 70-71 24.634272436498332 25.045989353422073 25.426516455609903 24.89322175446969 72-73 24.581808885353936 25.321554368821726 25.237018520622655 24.859618225201682 74-75 24.523065560731734 25.3781388478582 25.33024656289058 24.768549028519487 76-77 24.54101806612885 25.11583910919214 25.42818997841154 24.914952846267468 78-79 24.372230428360414 25.252471310078402 25.35853880240882 25.01675945915237 80-81 24.52107584485778 25.35709780542105 25.226918622953132 24.894907726768047 82-83 24.633401592305297 25.417224443227248 25.328813543913398 24.620560420554057 84-85 24.58826363855389 25.31888827773055 25.244052949546102 24.848795134169457 86-87 24.39492266317556 25.346227255086795 25.46254074807702 24.796309333660624 88-89 24.486312461575004 25.197423522178823 25.369419781103936 24.94684423514224 90-91 24.475590832151 25.294485642351155 25.384432248273086 24.845491277224756 92-93 24.572771100082104 25.098962787037536 25.60612043715785 24.722145675722512 94-95 24.502707236572924 25.054667539528836 25.527695189256523 24.914930034641717 96-97 24.27926696852633 25.05723079650382 25.644624168811102 25.018878066158745 98-99 24.300360068888676 25.010440690909526 25.641370932321028 25.047828307880778 100-101 24.538007455974125 24.974729122903113 25.522023306595543 24.96524011452722 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3.0 1 4.5 2 6.0 3 9.5 4 9.5 5 5.0 6 10.0 7 25.0 8 39.0 9 45.0 10 38.0 11 36.5 12 44.5 13 53.5 14 63.0 15 69.0 16 70.0 17 78.0 18 98.0 19 116.0 20 139.5 21 165.0 22 195.0 23 242.5 24 306.0 25 408.5 26 527.5 27 678.0 28 923.0 29 1207.5 30 1632.0 31 2283.0 32 3043.0 33 3966.0 34 5171.5 35 6608.5 36 8366.5 37 10512.0 38 13025.5 39 15655.5 40 18231.0 41 20919.5 42 23922.5 43 27608.0 44 31134.0 45 34301.0 46 37097.5 47 39591.0 48 42072.5 49 43892.0 50 44741.0 51 44903.0 52 44804.0 53 43956.5 54 42595.5 55 40489.0 56 37225.5 57 33666.5 58 30179.0 59 26161.5 60 22197.5 61 18531.5 62 14535.5 63 11146.5 64 8454.5 65 5990.5 66 4211.0 67 3139.0 68 2272.5 69 1661.0 70 1247.5 71 915.5 72 682.0 73 505.5 74 401.5 75 266.5 76 172.5 77 114.5 78 66.0 79 52.0 80 44.5 81 28.5 82 18.0 83 14.5 84 11.5 85 6.5 86 7.5 87 9.5 88 6.5 89 5.5 90 5.5 91 2.5 92 1.0 93 0.5 94 1.5 95 2.5 96 1.5 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00488580843086013 2 0.006590160209067151 3 6.817407112828088E-4 4 1.1362345188046814E-4 5 0.0030678332007726392 6 0.00488580843086013 7 0.005113055334621065 8 0.0044313146233382564 9 0.007385524372230428 10-11 0.006817407112828088 12-13 0.0036359504601749804 14-15 0.0 16-17 0.0 18-19 1.704351778207022E-4 20-21 0.0015339166003863196 22-23 5.113055334621066E-4 24-25 0.0014202931485058515 26-27 0.003749573912055448 28-29 0.002897398022951937 30-31 0.010566981024883536 32-33 5.113055334621066E-4 34-35 0.0 36-37 1.704351778207022E-4 38-39 2.8405862970117034E-4 40-41 0.0 42-43 2.8405862970117034E-4 44-45 3.976820815816384E-4 46-47 0.002670151119191001 48-49 0.0036359504601749804 50-51 0.003408703556414044 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0013066696966253836 64-65 0.0027837745710714693 66-67 0.0031246449267128733 68-69 0.007726394727871832 70-71 5.681172594023407E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.01806612884899443 82-83 0.01340756732189524 84-85 0.019088739915918647 86-87 0.017100329508010454 88-89 0.01579365981138507 90-91 0.015168730826042497 92-93 0.011078286558345644 94-95 0.02681513464379048 96-97 0.012555391432791729 98-99 0.014714237018520622 100-101 0.015282354277922964 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 880100.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.71746293422301 #Duplication Level Percentage of deduplicated Percentage of total 1 77.10223767511079 52.21167921909967 2 13.996675073912677 18.956386510400893 3 4.389191731536324 8.916747851745274 4 1.828550356908075 4.952991640691313 5 0.9108428059959643 3.08399819769677 6 0.5432684787997433 2.2073257845872 7 0.32486048655694566 1.5399109570049534 8 0.20853804233872492 1.1297333721958427 9 0.15686699050985353 0.9560371153908709 >10 0.5280089090997624 5.500270181571815 >50 0.009779583181727168 0.43876959403172794 >100 0.0011798660493661955 0.10614957558365698 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 5.681172594023406E-5 0.0 22-23 0.0 0.0 0.0 2.2724690376093625E-4 1.1362345188046812E-4 24-25 0.0 0.0 0.0 5.681172594023407E-4 1.1362345188046812E-4 26-27 0.0 0.0 0.0 7.385524372230429E-4 1.1362345188046812E-4 28-29 0.0 0.0 0.0 9.657993409839792E-4 1.1362345188046812E-4 30-31 0.0 0.0 0.0 0.0020452221338484265 1.1362345188046812E-4 32-33 0.0 0.0 0.0 0.004317691171457789 2.8405862970117034E-4 34-35 0.0 0.0 0.0 0.007953641631632768 3.408703556414044E-4 36-37 0.0 0.0 0.0 0.014998295648221792 3.408703556414044E-4 38-39 0.0 1.1362345188046812E-4 0.0 0.029712532666742415 3.408703556414044E-4 40-41 0.0 2.2724690376093625E-4 0.0 0.04556300420406772 3.408703556414044E-4 42-43 0.0 2.2724690376093625E-4 0.0 0.06368594477900238 3.408703556414044E-4 44-45 0.0 2.2724690376093625E-4 0.0 0.08516077718441087 3.976820815816385E-4 46-47 0.0 2.2724690376093625E-4 0.0 0.11004431314623339 4.544938075218725E-4 48-49 0.0 2.2724690376093625E-4 0.0 0.13737075332348597 4.544938075218725E-4 50-51 0.0 2.2724690376093625E-4 0.0 0.1722531530507897 4.544938075218725E-4 52-53 0.0 2.2724690376093625E-4 0.0 0.21111237359390977 5.681172594023407E-4 54-55 0.0 2.2724690376093625E-4 0.0 0.25372116804908534 5.681172594023407E-4 56-57 0.0 2.2724690376093625E-4 0.0 0.30195432337234407 6.249289853425747E-4 58-59 0.0 2.2724690376093625E-4 0.0 0.3575730030678332 6.817407112828088E-4 60-61 0.0 2.2724690376093625E-4 0.0 0.42052039540961256 6.817407112828088E-4 62-63 0.0 2.2724690376093625E-4 0.0 0.48914896034541533 9.08987615043745E-4 64-65 0.0 2.2724690376093625E-4 0.0 0.5593114418816043 9.08987615043745E-4 66-67 0.0 2.2724690376093625E-4 0.0 0.6412907624133621 9.08987615043745E-4 68-69 0.0 2.2724690376093625E-4 0.0 0.7364504033632542 9.08987615043745E-4 70-71 0.0 2.2724690376093625E-4 0.0 0.8563799568230883 9.08987615043745E-4 72-73 0.0 2.2724690376093625E-4 0.0 0.9865924326781047 0.0010794227928644473 74-75 0.0 2.2724690376093625E-4 0.0 1.1197591182820135 0.0012498579706851495 76-77 0.0 2.2724690376093625E-4 0.0 1.2595727758209294 0.0013634814225656176 78-79 0.0 2.2724690376093625E-4 0.0 1.420179525053971 0.0013634814225656176 80-81 0.0 2.2724690376093625E-4 0.0 1.6044767640040902 0.0014771048744460858 82-83 0.0 2.2724690376093625E-4 0.0 1.8057607090103396 0.001590728326326554 84-85 0.0 2.2724690376093625E-4 0.0 2.0246562890580617 0.001590728326326554 86-87 0.0 2.2724690376093625E-4 0.0 2.2625269855698216 0.001647540052266788 88-89 0.0 2.2724690376093625E-4 0.0 2.534143847290081 0.0017043517782070218 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGACG 55 4.961659E-5 43.182854 4 GTACATG 2500 0.0 34.96084 1 GTATCAA 785 0.0 34.491272 1 TACATGG 2500 0.0 33.82081 2 GGTATCA 605 0.0 30.620567 1 ACATGGG 2640 0.0 30.58785 3 TCAACGC 875 0.0 30.400726 4 CAACGCA 890 0.0 29.888353 5 ATCAACG 880 0.0 29.688211 3 AACGCAG 930 0.0 28.602837 6 TACACCG 165 8.403549E-8 25.90971 5 TATCAAC 1070 0.0 24.860409 2 CATGGGG 1985 0.0 24.169344 4 GTATTAG 160 1.8583451E-6 23.750568 1 GTATAAA 310 0.0 22.98442 1 TTACACC 370 0.0 21.824846 4 AGTACAT 1615 0.0 20.882853 2 TATGCCG 140 4.8497136E-4 20.35763 5 ACGCAGA 1310 0.0 20.30583 1 GTATTAC 235 1.1308293E-7 20.21325 1 >>END_MODULE