FastQCFastQC Report
Fri 10 Feb 2017
SRR2568641_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568641_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880100
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8000.033.880971
TCAACGC8000.032.66434
CAACGCA8050.032.4614145
AACGCAG8500.030.7428686
GTACATG22800.030.2415561
ATCAACG8650.030.2097553
GGTATCA5300.029.6080021
TACATGG22500.029.3733772
ACATGGG24150.027.1495483
TATCAAC10400.025.602142
ATACCGT950.001224565725.0061636
GAGTACA15750.024.7573571
GTGTAGC7300.023.450421
ACGCAGA10900.023.102027
ACCGGTC1452.334219E-522.930178
AGTACAT15600.022.2494772
GTATAGC3900.020.72791
CGCAGAG12250.020.5502458
GTATAAG2108.512652E-720.3795321
TGCACCG2108.582647E-720.362165
Produced by FastQC (version 0.11.5)