FastQCFastQC Report
Fri 10 Feb 2017
SRR2568638_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568638_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426083
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG13000.035.0783542
GTACATG13200.034.5468641
ACATGGG13250.032.261683
GGTATCA2700.031.6679571
GTATCAA3550.030.7759041
GTCTTAC804.4878747E-429.688711
CATGGGG10400.028.7718334
TGTAAGG1806.670234E-926.3899632
TACCGTG908.9422695E-426.3868687
GTATAGC1457.8652374E-726.2079641
ACCGTGC1109.46784E-525.9071068
CTAATAC1151.2805694E-424.780713
TACACTG2950.024.1506945
CCGGTAC1201.7092425E-423.7481823
ATGGGGG5500.023.3163975
AGTACAT7550.023.2790952
TCAACGC4500.023.2204444
CTAGAAC1852.5357258E-723.1063373
ATCAACG4350.022.929283
TAATACC1050.002194946922.6173134
Produced by FastQC (version 0.11.5)