FastQCFastQC Report
Fri 10 Feb 2017
SRR2568638_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568638_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426083
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGT250.00501543257.0105636
GTATCAA4350.049.2047961
GTATTAG608.21585E-539.63721
TCAACGC5550.036.8086244
TATCAAC6000.034.8766332
ATCAACG5900.034.625063
AACGCAG6050.034.5518576
CAACGCA5950.034.3340955
GGTATCA2500.032.3439521
GTGCTAT902.387127E-531.7097591
GTACATG14750.029.3449631
GTATAGT650.005801311729.2705461
ATACGGC650.005834711229.2361896
TACATGG14800.027.957012
ACATGGG15050.026.5165423
TTATGCT1109.450992E-525.9138934
GACTAGG950.001215610125.0340211
TTAGGAC950.001223964225.0046353
ACGCAGA8300.024.6129937
GAGTACA10550.023.8950331