##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568638_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 426083 Sequences flagged as poor quality 0 Sequence length 101 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.77583944912141 34.0 31.0 34.0 31.0 34.0 2 32.89583954299984 34.0 31.0 34.0 31.0 34.0 3 32.96529314710983 34.0 31.0 34.0 31.0 34.0 4 36.30617039403121 37.0 37.0 37.0 35.0 37.0 5 36.35321991255225 37.0 37.0 37.0 35.0 37.0 6 36.37316907738633 37.0 37.0 37.0 35.0 37.0 7 36.30602018855481 37.0 37.0 37.0 35.0 37.0 8 36.30369200367065 37.0 37.0 37.0 35.0 37.0 9 38.14474879307553 39.0 39.0 39.0 37.0 39.0 10-11 38.07182872820554 39.0 39.0 39.0 36.0 39.0 12-13 38.058886414149356 39.0 39.0 39.0 35.0 39.0 14-15 39.56684847787872 41.0 40.0 41.0 37.0 41.0 16-17 39.55456331278178 41.0 40.0 41.0 37.0 41.0 18-19 39.55845105296386 41.0 40.0 41.0 37.0 41.0 20-21 39.51487503608452 41.0 40.0 41.0 37.0 41.0 22-23 39.484889094378325 41.0 39.0 41.0 37.0 41.0 24-25 39.4517699603129 41.0 39.0 41.0 36.0 41.0 26-27 39.3538242548987 41.0 39.0 41.0 36.0 41.0 28-29 39.25853882928913 41.0 39.0 41.0 36.0 41.0 30-31 39.233384105913636 41.0 39.0 41.0 36.0 41.0 32-33 39.121640619315954 41.0 39.0 41.0 35.0 41.0 34-35 39.034726802055 40.5 39.0 41.0 35.0 41.0 36-37 38.951693684094415 40.0 38.5 41.0 35.0 41.0 38-39 38.80956761945443 40.0 38.0 41.0 35.0 41.0 40-41 38.727326600685785 40.0 38.0 41.0 35.0 41.0 42-43 38.587071063619064 40.0 38.0 41.0 34.5 41.0 44-45 38.46860236151172 40.0 38.0 41.0 34.0 41.0 46-47 38.28157776771192 40.0 38.0 41.0 34.0 41.0 48-49 38.12090719413823 40.0 37.0 41.0 33.0 41.0 50-51 38.0995815369306 40.0 37.0 41.0 33.5 41.0 52-53 38.15981862688725 40.0 37.0 41.0 34.0 41.0 54-55 37.9534421697181 40.0 36.5 41.0 34.0 41.0 56-57 37.73294053036615 39.0 36.0 41.0 33.0 41.0 58-59 37.44506586744836 39.0 35.5 41.0 33.0 41.0 60-61 37.25125503716412 39.0 35.0 41.0 33.0 41.0 62-63 36.9645080887996 38.0 35.0 40.5 33.0 41.0 64-65 36.63506992769014 37.0 35.0 40.0 33.0 41.0 66-67 36.31487409730029 37.0 35.0 39.5 32.0 41.0 68-69 35.9470654778529 36.0 35.0 39.0 32.0 41.0 70-71 35.561466897294665 36.0 35.0 38.5 32.0 40.5 72-73 35.175545609658215 35.0 35.0 37.0 31.0 39.5 74-75 34.83640041963655 35.0 35.0 37.0 31.0 39.0 76-77 33.175621885876694 34.5 32.0 35.5 28.0 37.0 78-79 34.14490251899278 35.0 34.0 36.0 30.5 37.0 80-81 34.10789212430442 35.0 34.0 35.5 31.0 37.0 82-83 33.99244865437016 35.0 34.0 35.0 31.0 36.5 84-85 33.81063548651319 35.0 34.0 35.0 31.0 36.0 86-87 33.69164341219903 35.0 34.0 35.0 31.0 36.0 88-89 33.60027271681808 35.0 34.0 35.0 31.0 36.0 90-91 33.54266070225754 35.0 34.0 35.0 31.0 35.0 92-93 33.48473184802022 35.0 34.0 35.0 30.0 35.0 94-95 33.43265044603986 35.0 34.0 35.0 30.0 35.0 96-97 33.37394592133458 35.0 34.0 35.0 30.0 35.0 98-99 33.31654748018578 35.0 34.0 35.0 30.0 35.0 100-101 32.09693416540909 34.5 32.0 35.0 26.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4614413448304404 1101 2 0.3523283522827043 1101 3 0.4172232620222829 1101 4 0.15465900009939304 1101 5 0.3209515276848265 1101 6 0.2644608727771143 1101 7 0.27299586125747766 1101 8 0.24850808512724143 1101 9 0.24984173166415502 1101 10-11 0.2663199631209423 1101 12-13 0.33499046972039537 1101 14-15 0.42359574781969656 1101 16-17 0.5273371322963456 1101 18-19 0.4115745376108393 1101 20-21 0.4857463898461205 1101 22-23 0.43454307709258444 1101 24-25 0.4823889046967764 1101 26-27 0.5127894219917124 1101 28-29 0.3965552307955278 1101 30-31 0.2725368751028654 1101 32-33 0.3321298469175886 1101 34-35 0.5388456049699997 1101 36-37 0.28019276867213705 1101 38-39 0.3627991660592613 1101 40-41 0.3501776685837612 1101 42-43 0.4176475479296684 1101 44-45 0.3136001906097263 1101 46-47 0.3706274456173304 1101 48-49 0.3256651903011729 1101 50-51 0.24806488389112502 1101 52-53 0.3090259225405063 1101 54-55 0.3887000382347736 1101 56-57 0.21688358923932327 1101 58-59 0.22597237713929275 1101 60-61 0.1706181358677057 1101 62-63 0.17120694856178176 1101 64-65 0.13208846897016713 1101 66-67 0.09631853351274344 1101 68-69 -0.05067345554861191 1101 70-71 0.10104659006879757 1101 72-73 0.010871438411982126 1101 74-75 0.05996175869100995 1101 76-77 0.0846557445626317 1101 78-79 0.5611615372032404 1101 80-81 0.4425591073308297 1101 82-83 0.25383206709874884 1101 84-85 0.3352666442785406 1101 86-87 0.4481838379239207 1101 88-89 0.38643611988871385 1101 90-91 0.28892115867447643 1101 92-93 0.15530335008066487 1101 94-95 0.12509860862154198 1101 96-97 0.07392193013829029 1101 98-99 0.09507567375533199 1101 100-101 8.061285065465995E-4 1102 1 0.4527612206068383 1102 2 0.220946364705064 1102 3 0.33362077755023734 1102 4 0.25018695040995453 1102 5 0.2736844469394839 1102 6 0.3056875808516466 1102 7 0.3218933767854324 1102 8 0.18195529009618383 1102 9 0.2939566384964536 1102 10-11 0.2416693420029361 1102 12-13 0.3894159355589082 1102 14-15 0.4775864310495095 1102 16-17 0.5249458279485211 1102 18-19 0.44201708419468844 1102 20-21 0.4706842780448781 1102 22-23 0.37897630690624595 1102 24-25 0.34575070593901813 1102 26-27 0.3528204778923296 1102 28-29 0.4335893922862084 1102 30-31 0.41494370740100095 1102 32-33 0.4939854369797061 1102 34-35 0.48050709565323046 1102 36-37 0.39048003575287993 1102 38-39 0.5609591039474608 1102 40-41 0.426940090944008 1102 42-43 0.48024071563153115 1102 44-45 0.4956498800507134 1102 46-47 0.5444395574185705 1102 48-49 0.5165114635930976 1102 50-51 0.4058987348228058 1102 52-53 0.5636221958324299 1102 54-55 0.5732186717751446 1102 56-57 0.3909674401710035 1102 58-59 0.4798776566423939 1102 60-61 0.5637004340043532 1102 62-63 0.3664476317915941 1102 64-65 0.4719953012683007 1102 66-67 0.4337331297860345 1102 68-69 0.41982343886132867 1102 70-71 0.3256894472116514 1102 72-73 0.3228512520765747 1102 74-75 0.3741387773245535 1102 76-77 0.2833281048110763 1102 78-79 0.1811071893771583 1102 80-81 0.32468643652337903 1102 82-83 0.5384904521919225 1102 84-85 0.5799095014213975 1102 86-87 0.4477412913400727 1102 88-89 0.31459605777691024 1102 90-91 0.44180935743224126 1102 92-93 0.5293716730620375 1102 94-95 0.46117003719297145 1102 96-97 0.4088598183370564 1102 98-99 0.5367294004634076 1102 100-101 0.4673123396866714 1103 1 0.2675774371503721 1103 2 0.16103507731254751 1103 3 0.20863591622045163 1103 4 0.2036204008156659 1103 5 0.041850851202532624 1103 6 0.13935144181809989 1103 7 0.21588483857542684 1103 8 0.10854787820324674 1103 9 0.22231288168405428 1103 10-11 0.25647117680976095 1103 12-13 0.17764864719751472 1103 14-15 0.31895587515754187 1103 16-17 0.3854429818785192 1103 18-19 0.3171495475591115 1103 20-21 0.30532458324046985 1103 22-23 0.3134447881948432 1103 24-25 0.2549754546370906 1103 26-27 0.27063263727225007 1103 28-29 0.23029975924757196 1103 30-31 0.18535820419160842 1103 32-33 0.09057393764580723 1103 34-35 0.0702264247073714 1103 36-37 0.4460503094600412 1103 38-39 0.3243908890994476 1103 40-41 0.3463455953645038 1103 42-43 0.2916507313757464 1103 44-45 0.47445574174382443 1103 46-47 0.33297125779401426 1103 48-49 0.1699986562780964 1103 50-51 0.2607151027530463 1103 52-53 0.39912540523051376 1103 54-55 0.4317402292415409 1103 56-57 0.39315497801768373 1103 58-59 0.3623750830684571 1103 60-61 0.441028004550553 1103 62-63 0.2622216719999031 1103 64-65 0.4051883495909365 1103 66-67 0.3451605550010015 1103 68-69 0.32765921456678626 1103 70-71 0.4313649346768287 1103 72-73 0.21394744318715198 1103 74-75 0.3240524869043 1103 76-77 0.40653795705765816 1103 78-79 0.27444844528123724 1103 80-81 0.3967692147721351 1103 82-83 0.24791881600443588 1103 84-85 0.3151114512821209 1103 86-87 0.1666199700369333 1103 88-89 0.27301391535230835 1103 90-91 0.13190563937482835 1103 92-93 0.16596789444994897 1103 94-95 0.3231129341289005 1103 96-97 0.26926614024574036 1103 98-99 0.34302633034108254 1103 100-101 0.40773410303897606 1104 1 0.3294355728246643 1104 2 0.23195317190752007 1104 3 0.14741085220987316 1104 4 0.15060416604455895 1104 5 0.1071275738608719 1104 6 0.17549983381607603 1104 7 0.040355168616784454 1104 8 0.15807518469434 1104 9 0.23760507942750309 1104 10-11 0.2585638303648139 1104 12-13 0.238092922822851 1104 14-15 0.2912941605181132 1104 16-17 0.10092299088220358 1104 18-19 0.12360195463826074 1104 20-21 0.20053715463688349 1104 22-23 0.13184466439416553 1104 24-25 0.16995744726531825 1104 26-27 0.15519201939430616 1104 28-29 0.2143041485444428 1104 30-31 -0.0609840484180566 1104 32-33 -0.15048920070145044 1104 34-35 0.04311688924128987 1104 36-37 0.06778295626232733 1104 38-39 0.11735183561192741 1104 40-41 0.0647447681508595 1104 42-43 0.08748160276372374 1104 44-45 0.1011615181710468 1104 46-47 0.05164476163464826 1104 48-49 0.1365021311381156 1104 50-51 0.19332462415086837 1104 52-53 0.2211759946905758 1104 54-55 0.26714159667633197 1104 56-57 0.23805242040815955 1104 58-59 0.18838940455631104 1104 60-61 0.32439158464115536 1104 62-63 0.3509255632803985 1104 64-65 0.13685037373207365 1104 66-67 0.3556836128778187 1104 68-69 0.39286911299395655 1104 70-71 0.44248237650457867 1104 72-73 0.3941759117164594 1104 74-75 0.34677272550197813 1104 76-77 0.23983611974300345 1104 78-79 0.17626254730425472 1104 80-81 0.3168087465804632 1104 82-83 0.23765602092270655 1104 84-85 0.3002377842496742 1104 86-87 0.22322712796721333 1104 88-89 0.22326151671411765 1104 90-91 0.14443342418673666 1104 92-93 0.3269122966896134 1104 94-95 0.2509427644656981 1104 96-97 0.2788642100805774 1104 98-99 0.283531662211324 1104 100-101 0.16957957727999684 1105 1 0.45346693118438 1105 2 0.0999616145428277 1105 3 0.21863051148923773 1105 4 0.2512901301633619 1105 5 0.27118606926265443 1105 6 0.30881055294769055 1105 7 0.2824628122169699 1105 8 0.29550168853875647 1105 9 0.2661743542654378 1105 10-11 0.2764478948906728 1105 12-13 0.338401983579665 1105 14-15 0.37267890347001753 1105 16-17 0.3882326546844084 1105 18-19 0.3762440472057236 1105 20-21 0.4388380742810867 1105 22-23 0.49262410054673467 1105 24-25 0.3413014846541387 1105 26-27 0.5577361170876571 1105 28-29 0.5402652521174858 1105 30-31 0.38695052115830464 1105 32-33 0.6078441326318753 1105 34-35 0.6375236433495317 1105 36-37 0.7053526833842909 1105 38-39 0.6010507652063808 1105 40-41 0.3689623167713947 1105 42-43 0.6392040511279617 1105 44-45 0.6133656490318984 1105 46-47 0.7612671044659436 1105 48-49 0.6624029301732435 1105 50-51 0.7757194681128752 1105 52-53 0.7680024683827256 1105 54-55 0.59441432394906 1105 56-57 0.5925978749536114 1105 58-59 0.6539894347085848 1105 60-61 0.7448612062301763 1105 62-63 0.5116333877941912 1105 64-65 0.5169498762196341 1105 66-67 0.5886852712526149 1105 68-69 0.5119065018074664 1105 70-71 0.5449058651220966 1105 72-73 0.71383519107723 1105 74-75 0.47036687596180116 1105 76-77 0.453035275479472 1105 78-79 0.44340034317339416 1105 80-81 0.4651390646869089 1105 82-83 0.5400154359686908 1105 84-85 0.5662900984363262 1105 86-87 0.4993352238514248 1105 88-89 0.5218518981403193 1105 90-91 0.6385160414036832 1105 92-93 0.5591413031723533 1105 94-95 0.47441630584968664 1105 96-97 0.5329837638269979 1105 98-99 0.42833793388326313 1105 100-101 0.336690989913798 1106 1 0.23307934034278333 1106 2 0.16251533807178475 1106 3 0.20568997855183113 1106 4 0.22867545484883323 1106 5 0.08264187921810873 1106 6 0.2277368638017947 1106 7 0.2610437296194874 1106 8 0.12638392656329245 1106 9 0.15087389321988098 1106 10-11 0.2163947574365821 1106 12-13 0.10679301272712394 1106 14-15 0.2324663536536562 1106 16-17 0.3882795406733166 1106 18-19 0.2848280903408309 1106 20-21 0.32411587376534357 1106 22-23 0.22143163356228968 1106 24-25 0.3449468702913947 1106 26-27 0.2942329522834086 1106 28-29 0.2584101298231971 1106 30-31 0.4889392685209657 1106 32-33 0.39423156718684993 1106 34-35 0.1490849467800217 1106 36-37 0.3207312877446853 1106 38-39 0.22893229994108566 1106 40-41 0.1423467037370898 1106 42-43 0.21056452623931676 1106 44-45 0.18515591237486717 1106 46-47 0.2942474904939161 1106 48-49 0.2992553174515038 1106 50-51 0.3912660806001824 1106 52-53 0.4738202381725145 1106 54-55 0.3085504496035085 1106 56-57 0.29730991683842944 1106 58-59 0.12329474063966472 1106 60-61 0.037828307894635316 1106 62-63 0.17646498895071971 1106 64-65 0.13859557443014836 1106 66-67 0.2892205529592644 1106 68-69 0.22980522807142734 1106 70-71 0.18400096553625644 1106 72-73 0.12356403377473413 1106 74-75 0.2120485200971487 1106 76-77 -0.01604732200431158 1106 78-79 0.047706039821044044 1106 80-81 0.11445140351631267 1106 82-83 0.21240349567018058 1106 84-85 0.22748803545280794 1106 86-87 0.26461614906827435 1106 88-89 0.03885197628363102 1106 90-91 0.18557785276572503 1106 92-93 0.15283513766480894 1106 94-95 0.15979569164323948 1106 96-97 0.27276261824599857 1106 98-99 0.2569305727830056 1106 100-101 0.4949272952978703 1107 1 0.19105173444082624 1107 2 0.24154913150348278 1107 3 0.12215832695734719 1107 4 0.07913951957991117 1107 5 0.13010737184067267 1107 6 0.12322710654334657 1107 7 0.1615672898289091 1107 8 0.14065094227009922 1107 9 0.27295861478103944 1107 10-11 0.23823554753653298 1107 12-13 0.25236059959052426 1107 14-15 0.1857386049625589 1107 16-17 0.31392804138726405 1107 18-19 0.2786524596887574 1107 20-21 0.15912301322274658 1107 22-23 0.18399112904063486 1107 24-25 0.1723564371643107 1107 26-27 0.31201020121248746 1107 28-29 0.05710717884747396 1107 30-31 0.1510109010768943 1107 32-33 0.15898049626824218 1107 34-35 0.15624820237259485 1107 36-37 0.13684861282798266 1107 38-39 0.301695269955367 1107 40-41 0.15186598027207054 1107 42-43 -0.04219011440799392 1107 44-45 0.041944346453881565 1107 46-47 0.0531599131497984 1107 48-49 0.08738596952195365 1107 50-51 0.05809735142359784 1107 52-53 0.17029215630673633 1107 54-55 0.19782341485814925 1107 56-57 0.3085069658627049 1107 58-59 0.11048536415227517 1107 60-61 0.02464410989367849 1107 62-63 0.20584980570463784 1107 64-65 0.11488067676237534 1107 66-67 0.15846139065559584 1107 68-69 0.20208628471112888 1107 70-71 0.056371265393472925 1107 72-73 0.15869611373665293 1107 74-75 0.28174747297672553 1107 76-77 0.13933106923795435 1107 78-79 -0.03952028097857152 1107 80-81 0.18145521122693253 1107 82-83 0.1260398593065446 1107 84-85 0.2003640468759471 1107 86-87 0.16337864311872607 1107 88-89 0.19055949651209403 1107 90-91 0.17031726257057045 1107 92-93 0.05708906436638728 1107 94-95 0.1691245826475125 1107 96-97 0.3507076444240127 1107 98-99 0.2647185308981861 1107 100-101 0.3550593752597919 1108 1 0.1793076844993564 1108 2 0.04230995865916043 1108 3 0.060901986731053626 1108 4 0.1482089694433526 1108 5 0.08087795319065094 1108 6 -0.0729394950953548 1108 7 -0.07877605781057895 1108 8 -0.05108725515479762 1108 9 0.007254361952327315 1108 10-11 0.07625251983461823 1108 12-13 0.08204748995337496 1108 14-15 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90-91 -0.3077671530138417 2316 92-93 -0.42407976680244985 2316 94-95 -0.38136243033949313 2316 96-97 -0.4069222257058627 2316 98-99 -0.5945347158550547 2316 100-101 -0.2938042611038405 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 0.0 11 0.0 12 0.0 13 0.0 14 7.0 15 19.0 16 45.0 17 101.0 18 157.0 19 257.0 20 332.0 21 498.0 22 693.0 23 890.0 24 1080.0 25 1443.0 26 1834.0 27 2481.0 28 3269.0 29 4015.0 30 5022.0 31 6634.0 32 8418.0 33 11449.0 34 17152.0 35 28541.0 36 58036.0 37 125195.0 38 130068.0 39 18444.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.80272532012535 15.259210572645863 10.514665320829568 26.423398786399222 2 17.68811684635137 18.014438991385003 36.340164406218214 27.957279756045416 3 20.947561860013188 20.996848031956215 26.927382693043374 31.12820741498722 4 13.922874181790872 13.519901052142421 33.56364839714328 38.99357636892343 5 15.864984052402937 35.8739963809868 32.732824355817996 15.528195210792264 6 38.037332507164436 31.921570284206798 15.586192652260348 14.454904556368426 7 33.1315967396612 29.038331517860495 18.46758441948691 19.3624873229914 8 29.740919022819494 32.98817366569424 17.956360615185304 19.314546696300955 9 28.03491338542021 13.828995759042254 16.756594372457947 41.379496483079585 10-11 27.7524566809753 23.779756526310603 26.1192068212062 22.3485799715079 12-13 27.884121168880245 20.49718012687669 26.305086098248466 25.313612605994606 14-15 24.58065682038476 22.274533365564924 23.03870372673869 30.10610608731163 16-17 25.782065935510218 25.216683134506656 23.58648432347688 25.414766606506245 18-19 25.677274146117067 24.745061408223282 24.65259116181589 24.92507328384376 20-21 26.16990116949045 23.852160259855474 24.688030266403494 25.28990830425058 22-23 25.868434084438945 23.83314987924888 24.446880067967978 25.851535968344198 24-25 25.6914732575578 23.969390940262812 24.523860374621844 25.815275427557545 26-27 25.637962556591088 24.247975159769343 24.520339933768774 25.593722349870802 28-29 25.639497086207424 24.277771388739595 24.463887982688576 25.618843542364406 30-31 25.89448534675169 24.09025940955166 24.328593255304718 25.686661988391933 32-33 25.543262697643414 24.24199041031912 24.488069225948937 25.72667766608853 34-35 25.734070591879988 24.269567197001525 24.487247789749883 25.509114421368608 36-37 25.877352534600067 24.078524606708083 24.457558738555633 25.586564120136217 38-39 25.80964272219262 24.207490089959 24.493232539200108 25.48963464864827 40-41 25.84508182678023 24.140836409807477 24.441364710631497 25.572717052780796 42-43 25.650636143662148 24.075121513883445 24.552845337645483 25.72139700480892 44-45 25.596538702553257 24.087560404897637 24.514707228403857 25.80119366414525 46-47 25.694406958268694 24.11220349086915 24.442890235001162 25.750499315860992 48-49 25.65098818774746 24.08556548841423 24.389262186006015 25.8741841378323 50-51 25.625875709662203 24.333873916584327 24.362858879607963 25.677391494145507 52-53 25.74122882161457 24.191178714006426 24.408976654783224 25.65861580959578 54-55 25.534930987624477 24.10551465324831 24.549676940877717 25.809877418249492 56-57 25.489282604562963 24.30019503242326 24.521044021939385 25.68947834107439 58-59 25.747565615150098 24.224740250139057 24.42153289382585 25.60616124088499 60-61 25.83287763182291 24.288812273664988 24.319205413029856 25.559104681482246 62-63 25.635353799683863 24.418685157651765 24.272234302592345 25.673726740072027 64-65 25.52531091506075 24.303712204956334 24.51376616183308 25.657210718149837 66-67 25.48893056047765 24.285174484783482 24.360746615096122 25.865148339642747 68-69 25.580931414771303 24.172168333399828 24.497691764280667 25.749208487548202 70-71 25.458068028999044 23.935007967931128 24.538411530147883 26.068512472921938 72-73 25.522726792667154 24.27660807870767 24.292802106631804 25.907863021993364 74-75 25.307627856544386 24.298200115939853 24.559651523294757 25.834520504221008 76-77 25.50629806868615 24.022666945172656 24.559182131181014 25.91185285496018 78-79 25.41523599861999 24.13496900838569 24.438078965835295 26.011716027159025 80-81 25.470272223956364 24.32354729008198 24.249265988082133 25.95691449787952 82-83 25.477899845804693 24.158673310129718 24.56927406162649 25.7941527824391 84-85 25.48118559060089 24.13966292952312 24.408272566612606 25.97087891326338 86-87 25.271367315757725 24.385389701067634 24.470232325626696 25.87301065754794 88-89 25.35151601917936 24.201505340508774 24.40885930675479 26.038119333557074 90-91 25.205652419833697 24.11912702454686 24.510013307266423 26.165207248353024 92-93 25.272423448013647 24.098121727456856 24.665616792972262 25.96383803155723 94-95 25.13888139165374 24.034049703930922 24.777214768014684 26.04985413640065 96-97 24.98468608228913 24.021610812916734 24.765832009256414 26.227871095537726 98-99 25.031273249578135 23.89581372643358 24.67312706679215 26.39978595719613 100-101 25.029425190779676 24.0955641011499 24.810217787483822 26.064792920586605 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.5 3 1.5 4 2.0 5 2.0 6 2.5 7 5.5 8 13.0 9 15.0 10 13.5 11 13.5 12 16.0 13 18.5 14 20.0 15 24.5 16 25.0 17 22.5 18 35.0 19 46.5 20 48.0 21 57.0 22 70.5 23 86.0 24 103.5 25 129.0 26 150.0 27 183.5 28 260.0 29 343.5 30 441.0 31 565.5 32 810.5 33 1130.0 34 1406.5 35 1792.0 36 2377.0 37 3085.0 38 3909.5 39 4775.0 40 5695.0 41 6803.5 42 8176.5 43 9992.5 44 11817.5 45 13463.5 46 15069.5 47 16712.0 48 18542.0 49 19974.0 50 20628.0 51 21461.0 52 22710.5 53 23707.0 54 24101.0 55 23380.0 56 21786.0 57 20113.5 58 18635.5 59 16776.0 60 14298.0 61 12028.0 62 9696.5 63 7279.0 64 5379.5 65 3912.5 66 2886.0 67 2196.0 68 1691.5 69 1277.0 70 977.5 71 749.5 72 555.0 73 421.0 74 337.0 75 267.0 76 210.5 77 144.0 78 92.0 79 51.0 80 28.5 81 22.5 82 16.5 83 8.5 84 3.0 85 3.0 86 3.0 87 3.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018306292435980785 2 0.10091930445476585 3 0.0 4 0.0 5 0.0 6 0.0046939211374309705 7 0.026990046540228078 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0014081763412292908 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 3.520440853073227E-4 64-65 4.6939211374309703E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 5.867401421788713E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 426083.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.77399444086704 #Duplication Level Percentage of deduplicated Percentage of total 1 81.56732668843011 59.35980178980199 2 12.02878048799352 17.507648087273004 3 3.1522032618575384 6.881952679647103 4 1.2758807712740272 3.7140376062362077 5 0.6285368161318308 2.2870567381529057 6 0.3743952198297909 1.6347741387948236 7 0.22675492183679372 1.1551302994833046 8 0.16666047647369514 0.9702838870727154 9 0.1095015220322742 0.7171976840078882 >10 0.46086998437571103 5.302212518810089 >50 0.008440574787825932 0.4091878920973072 >100 6.4927497688607E-4 0.060716678622673693 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.346960568715485E-4 0.0 12-13 0.0 0.0 0.0 2.346960568715485E-4 0.0 14-15 0.0 0.0 0.0 2.346960568715485E-4 2.346960568715485E-4 16-17 0.0 0.0 0.0 2.346960568715485E-4 4.69392113743097E-4 18-19 0.0 0.0 0.0 2.346960568715485E-4 4.69392113743097E-4 20-21 0.0 0.0 0.0 2.346960568715485E-4 4.69392113743097E-4 22-23 0.0 0.0 0.0 3.5204408530732276E-4 4.69392113743097E-4 24-25 0.0 0.0 0.0 4.69392113743097E-4 4.69392113743097E-4 26-27 0.0 0.0 0.0 9.38784227486194E-4 7.040881706146455E-4 28-29 0.0 0.0 0.0 0.001408176341229291 7.040881706146455E-4 30-31 0.0 0.0 0.0 0.004107180995252099 7.040881706146455E-4 32-33 0.0 0.0 0.0 0.0057500533933529385 7.040881706146455E-4 34-35 0.0 0.0 0.0 0.009857234388605036 0.0011734802843577426 36-37 0.0 0.0 0.0 0.015020547639779103 0.0011734802843577426 38-39 0.0 0.0 0.0 0.032505403876709464 0.0011734802843577426 40-41 0.0 0.0 0.0 0.05773522999040093 0.0011734802843577426 42-43 0.0 0.0 0.0 0.08237831596191353 0.0011734802843577426 44-45 0.0 0.0 0.0 0.10608261770593992 0.0011734802843577426 46-47 0.0 0.0 0.0 0.1370624972129843 0.0011734802843577426 48-49 0.0 0.0 0.0 0.17027198926030845 0.0011734802843577426 50-51 0.0 0.0 0.0 0.20711927018914156 0.0011734802843577426 52-53 0.0 0.0 0.0 0.25311969733596507 0.0015255243696650654 54-55 0.0 0.0 0.0 0.2986507323690455 0.001877568454972388 56-57 0.0 0.0 0.0 0.3540390017907309 0.001877568454972388 58-59 0.0 0.0 0.0 0.427264171534654 0.001877568454972388 60-61 0.0 0.0 0.0 0.5024842577619854 0.001877568454972388 62-63 0.0 0.0 0.0 0.5854493138660777 0.0021122645118439366 64-65 0.0 0.0 0.0 0.6763940359038028 0.0021122645118439366 66-67 0.0 0.0 0.0 0.7895175353158892 0.0021122645118439366 68-69 0.0 0.0 0.0 0.9272841206994882 0.0021122645118439366 70-71 0.0 0.0 0.0 1.0763161168129214 0.0021122645118439366 72-73 0.0 0.0 0.0 1.2300420340637856 0.0023469605687154852 74-75 0.0 0.0 0.0 1.407589601087112 0.0023469605687154852 76-77 0.0 0.0 0.0 1.5946423584137364 0.0023469605687154852 78-79 0.0 0.0 0.0 1.8167821762426568 0.0023469605687154852 80-81 0.0 0.0 0.0 2.076238667114154 0.0023469605687154852 82-83 0.0 0.0 0.0 2.367261777634874 0.0030510487393301303 84-85 0.0 0.0 0.0 2.6726013476247585 0.0030510487393301303 86-87 0.0 0.0 0.0 2.995895165965317 0.0030510487393301303 88-89 0.0 0.0 0.0 3.3770415623247114 0.0030510487393301303 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACCGT 25 0.005015432 57.010563 6 GTATCAA 435 0.0 49.204796 1 GTATTAG 60 8.21585E-5 39.6372 1 TCAACGC 555 0.0 36.808624 4 TATCAAC 600 0.0 34.876633 2 ATCAACG 590 0.0 34.62506 3 AACGCAG 605 0.0 34.551857 6 CAACGCA 595 0.0 34.334095 5 GGTATCA 250 0.0 32.343952 1 GTGCTAT 90 2.387127E-5 31.709759 1 GTACATG 1475 0.0 29.344963 1 GTATAGT 65 0.0058013117 29.270546 1 ATACGGC 65 0.0058347112 29.236189 6 TACATGG 1480 0.0 27.95701 2 ACATGGG 1505 0.0 26.516542 3 TTATGCT 110 9.450992E-5 25.913893 4 GACTAGG 95 0.0012156101 25.034021 1 TTAGGAC 95 0.0012239642 25.004635 3 ACGCAGA 830 0.0 24.612993 7 GAGTACA 1055 0.0 23.895033 1 >>END_MODULE