FastQCFastQC Report
Fri 10 Feb 2017
SRR2568635_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568635_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24251
Sequences flagged as poor quality0
Sequence length101
%GC39

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.9731557461547977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1530.6309018184817121No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.5979134881035834No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA730.30101851470042473No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC510.21030060616057067No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA470.19380644097150632No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT470.19380644097150632No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA450.18555935837697415No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA430.17731227578244196No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG430.17731227578244196No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC420.17318873448517588No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG420.17318873448517588No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC410.16906519318790977No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA380.1566945692961115No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG380.1566945692961115No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT380.1566945692961115No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.14020040410704712No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG330.13607686280978104No Hit
CCCTAGATGACACATGAGCAAAAGCCCACTTCGCCATCATATTCGTAGGA320.13195332151251496No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT320.13195332151251496No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT320.13195332151251496No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.13195332151251496No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG320.13195332151251496No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC310.12782978021524885No Hit
CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG290.11958269762071666No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT280.11545915632345058No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG280.11545915632345058No Hit
ACCTAAAACCTGGTGAACTACGACTGCTAGAAGTTGATAACCGAGTCGTT280.11545915632345058No Hit
CATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCCCAGGAAA270.11133561502618448No Hit
GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG260.10721207372891839No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA260.10721207372891839No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT260.10721207372891839No Hit
GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG250.10308853243165231No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT250.10308853243165231No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.10308853243165231No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGT201.7094793E-595.000014
ATGGGTA156.5394E-494.999995
GTATGGC156.5394E-494.999999
TGGGTAT156.5394E-494.999996
AGGCTTA200.002049603771.256
GGTATGG200.002049603771.258
GGGTATG250.004962360557.07
CTTACTA250.004962360557.09
AACGCAG2650.043.018876
GTATCAA2950.040.254241
GTACATG2500.038.01
TACATGG2500.038.02
ACATGGG2500.036.13
TCAACGC3200.035.6254
CAACGCA3200.035.6255
GGGAAGC958.2427505E-735.07
ATCAACG3400.034.926473
CATGGGG550.002530351534.5454564
TATCAAC3450.034.4202882
ACGCAGA3350.034.029857
Produced by FastQC (version 0.11.5)