FastQCFastQC Report
Fri 10 Feb 2017
SRR2568635_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568635_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24251
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT7272.997814523112449No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3191.3154096738278835No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT2741.1298503154509092RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT2310.9525380396684673RNA PCR Primer, Index 17 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1990.8205847181559524No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG1760.7257432683188322RNA PCR Primer, Index 17 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.6968784792379695No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT920.37936579934848047RNA PCR Primer, Index 17 (96% over 26bp)
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG620.2556595604304977TruSeq Adapter, Index 12 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.20205352356603853No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT480.19792998226877243No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG480.19792998226877243No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG470.19380644097150632No Hit
CCCTAGATGACACATGAGCAAAAGCCCACTTCGCCATCATATTCGTAGGA460.18968289967424024No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.18968289967424024No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA450.18555935837697415No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC400.1649416518906437No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT380.1566945692961115No Hit
GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG370.15257102799884542No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT360.1484474867015793No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG360.1484474867015793No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.1484474867015793No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA350.14432394540431323No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA350.14432394540431323No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA340.14020040410704712No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA330.13607686280978104RNA PCR Primer, Index 17 (96% over 28bp)
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC320.13195332151251496No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA300.12370623891798276No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT290.11958269762071666No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT290.11958269762071666No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT290.11958269762071666No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG280.11545915632345058No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT280.11545915632345058No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG270.11133561502618448No Hit
GCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAAT270.11133561502618448No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT270.11133561502618448No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC270.11133561502618448No Hit
GTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGG270.11133561502618448No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG270.11133561502618448No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.10721207372891839No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA250.10308853243165231No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCAAGC156.5394E-494.999997
CACCAAG200.002049603771.256
ATCACCA200.002049603771.254
CTATCAC200.002049603771.252
AGATGGA250.004962360557.06
TATCACC250.004962360557.03
AGTAATG459.43959E-442.2222253
TGGGAAG500.00158481538.06
GGTATCA1650.037.4242441
CATGGGA1152.2937456E-937.1739124
GTACATG2200.036.7045441
AGTACAT1404.0927262E-1033.9285742
TACATGG2400.033.645832
GTATCAA2700.033.4259261
ACATGGG2350.032.3404243
GAGTACA1656.730261E-1131.6666661
AACGCAG2900.029.4827586
ATCAACG3250.027.769233
TCAACGC3200.026.7187524
TATCAAC3400.026.5441192
Produced by FastQC (version 0.11.5)