##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568635_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 24251 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.57906890437508 34.0 31.0 34.0 31.0 34.0 2 32.684672796998065 34.0 31.0 34.0 31.0 34.0 3 32.7259494453837 34.0 31.0 34.0 31.0 34.0 4 36.169147664013856 37.0 35.0 37.0 35.0 37.0 5 36.23467073522741 37.0 37.0 37.0 35.0 37.0 6 36.319079625582454 37.0 37.0 37.0 35.0 37.0 7 36.23817574533009 37.0 37.0 37.0 35.0 37.0 8 36.20716671477465 37.0 37.0 37.0 35.0 37.0 9 37.974227866892086 39.0 39.0 39.0 35.0 39.0 10-11 37.94018803348315 39.0 38.5 39.0 35.0 39.0 12-13 37.94152818440477 39.0 39.0 39.0 35.0 39.0 14-15 39.26947342377633 41.0 39.0 41.0 36.0 41.0 16-17 39.32050224733001 41.0 39.0 41.0 36.0 41.0 18-19 39.49484557337841 41.0 39.5 41.0 36.5 41.0 20-21 39.4812997402169 41.0 39.0 41.0 37.0 41.0 22-23 39.38711805698734 41.0 39.0 41.0 35.5 41.0 24-25 39.30427611232527 41.0 39.0 41.0 35.0 41.0 26-27 39.00911302626696 41.0 38.5 41.0 35.0 41.0 28-29 38.822440311739726 40.5 38.0 41.0 35.0 41.0 30-31 38.47872252690611 40.0 37.5 41.0 35.0 41.0 32-33 37.9601872087749 40.0 35.5 41.0 34.0 41.0 34-35 37.593130180198756 40.0 35.0 41.0 33.0 41.0 36-37 37.26883427487526 40.0 35.0 41.0 33.0 41.0 38-39 36.66941569419818 40.0 35.0 41.0 31.0 41.0 40-41 36.27965857078058 40.0 35.0 41.0 29.0 41.0 42-43 35.845965114840624 40.0 35.0 41.0 24.5 41.0 44-45 35.298565007628554 40.0 35.0 41.0 19.5 41.0 46-47 34.603686445919756 40.0 35.0 41.0 12.5 41.0 48-49 34.03888499443322 39.5 35.0 41.0 9.0 41.0 50-51 33.575914395282666 39.0 33.5 41.0 8.0 41.0 52-53 33.56478083378005 39.0 34.0 41.0 7.5 41.0 54-55 33.283039874644345 39.0 34.0 41.0 7.0 41.0 56-57 32.902993690981816 39.0 33.0 41.0 7.0 41.0 58-59 32.50608222341347 38.5 31.5 41.0 7.0 41.0 60-61 32.12364438579853 38.5 30.5 41.0 7.0 41.0 62-63 31.626633953239043 38.0 28.0 41.0 7.0 41.0 64-65 31.145251742196198 37.0 26.0 40.5 7.0 41.0 66-67 30.532679064780837 37.0 22.0 40.0 7.0 41.0 68-69 29.764339614861242 36.5 19.5 39.5 7.0 41.0 70-71 29.21729000865944 36.0 16.5 39.0 7.0 41.0 72-73 28.773596965073605 35.0 15.0 39.0 7.0 41.0 74-75 28.420642447734114 35.0 15.0 37.0 7.0 40.0 76-77 27.049977320522864 33.5 12.5 36.0 7.0 39.0 78-79 27.774174260855226 35.0 15.0 36.0 7.0 39.0 80-81 27.637458249144366 35.0 15.0 36.0 7.0 38.0 82-83 27.440208651189643 35.0 15.0 36.0 7.0 37.0 84-85 27.138530369881654 35.0 15.0 35.0 7.0 37.0 86-87 26.987113933446043 35.0 13.5 35.0 7.0 36.0 88-89 26.831532720300196 35.0 12.0 35.0 7.0 36.0 90-91 26.718011628386456 35.0 11.0 35.0 7.0 36.0 92-93 26.635087212898437 35.0 11.0 35.0 7.0 36.0 94-95 26.551956620345553 35.0 10.5 35.0 7.0 35.0 96-97 26.481815182879053 34.5 10.0 35.0 7.0 35.0 98-99 26.444785782029605 34.0 10.0 35.0 7.0 35.0 100-101 25.665230299781452 32.5 10.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9298358864744927 1101 2 0.7923930584233787 1101 3 0.9875698248001754 1101 4 0.564878156590737 1101 5 0.6091072054997184 1101 6 0.5554696545274709 1101 7 0.6761338907871419 1101 8 0.7418529559891809 1101 9 0.8929002547140357 1101 10-11 0.9697278386772155 1101 12-13 0.961658434085642 1101 14-15 1.1696061639216282 1101 16-17 1.345319370505102 1101 18-19 1.08545404467133 1101 20-21 0.9465410293303478 1101 22-23 0.7952815670936602 1101 24-25 0.7990137670504609 1101 26-27 0.7954066546098701 1101 28-29 0.15465562643921515 1101 30-31 -0.40413700470080016 1101 32-33 0.2599443940694357 1101 34-35 0.15453498737802107 1101 36-37 0.49797672031019147 1101 38-39 0.8871414342326673 1101 40-41 0.9836495007353108 1101 42-43 1.0749936600316943 1101 44-45 1.5159374688689553 1101 46-47 1.100372578659595 1101 48-49 0.269362817014283 1101 50-51 1.2904936837261474 1101 52-53 1.558234021544905 1101 54-55 1.581374664036069 1101 56-57 2.7013191515656914 1101 58-59 2.47400501064525 1101 60-61 2.219517238796975 1101 62-63 3.074560702915708 1101 64-65 2.4763339068610755 1101 66-67 2.2672871902459413 1101 68-69 2.5086931993404598 1101 70-71 2.666611503988463 1101 72-73 3.36299730125938 1101 74-75 2.8882738589779002 1101 76-77 2.612636203913592 1101 78-79 2.788510635636296 1101 80-81 3.659645433110999 1101 82-83 3.2854890389785183 1101 84-85 2.7680469855215364 1101 86-87 1.9459118480964825 1101 88-89 2.207002214401548 1101 90-91 2.5052697781328632 1101 92-93 2.859582444765749 1101 94-95 1.8584088051629095 1101 96-97 2.8669871411320216 1101 98-99 3.7897043771974452 1101 100-101 2.832738238543218 1102 1 0.39160059235684486 1102 2 0.31298129371749184 1102 3 0.622863942447232 1102 4 -0.2998277257622064 1102 5 0.07675426432324883 1102 6 0.36429318393923893 1102 7 0.3261338907871405 1102 8 0.39185295598917946 1102 9 0.2517237841258009 1102 10-11 0.4491396033831023 1102 12-13 0.5616584340856363 1102 14-15 0.8857826345098587 1102 16-17 0.9806134881521587 1102 18-19 0.9133952211419185 1102 20-21 0.7009527940362332 1102 22-23 0.8393992141524862 1102 24-25 0.6269549435210493 1102 26-27 0.8145243016686976 1102 28-29 1.0590673911450992 1102 30-31 1.2870394658874318 1102 32-33 1.595238511716488 1102 34-35 1.9898291050250805 1102 36-37 2.1376826026631335 1102 38-39 2.940082610703257 1102 40-41 2.7807083242647224 1102 42-43 3.4205818953258103 1102 44-45 4.167408057104254 1102 46-47 3.076843166894882 1102 48-49 2.9796569346613353 1102 50-51 2.696376036667324 1102 52-53 1.6449987274272502 1102 54-55 2.066668781683127 1102 56-57 2.5807309162715697 1102 58-59 2.732828540057021 1102 60-61 3.2371642976205095 1102 62-63 4.177501879386302 1102 64-65 3.2763339068610726 1102 66-67 4.6702283667165325 1102 68-69 5.866046140516932 1102 70-71 5.847493856929635 1102 72-73 4.799762007141734 1102 74-75 5.152979741330842 1102 76-77 4.2405773803841775 1102 78-79 3.6017459297539425 1102 80-81 3.2743513154639423 1102 82-83 3.372253744860867 1102 84-85 4.019517573756829 1102 86-87 4.0562059657435405 1102 88-89 4.915825743813311 1102 90-91 4.528799189897569 1102 92-93 4.363994209471635 1102 94-95 4.614291158104084 1102 96-97 3.5405165528967295 1102 98-99 3.113233788962148 1102 100-101 2.4415617679549797 1103 1 0.5416005923568505 1103 2 0.7129812937174975 1103 3 0.17286394244723624 1103 4 0.5001722742377979 1103 5 -0.22324573567675543 1103 6 -0.13570681606076107 1103 7 -0.12386610921285524 1103 8 0.04185295598917804 1103 9 -0.24827621587419912 1103 10-11 -0.6758603966168977 1103 12-13 -0.6883415659143566 1103 14-15 0.11078263450986015 1103 16-17 0.38061348815215723 1103 18-19 0.08839522114192278 1103 20-21 0.42595279403623465 1103 22-23 -0.13560078584751523 1103 24-25 -0.1230450564789507 1103 26-27 0.414524301668699 1103 28-29 -0.9409326088549008 1103 30-31 -0.9629605341125682 1103 32-33 -0.8047614882835035 1103 34-35 -1.2601708949749266 1103 36-37 -1.4123173973368637 1103 38-39 -0.7599173892967457 1103 40-41 -1.194291675735279 1103 42-43 -1.2294181046741883 1103 44-45 -0.4825919428957448 1103 46-47 -1.1231568331051136 1103 48-49 -2.120343065338659 1103 50-51 -1.578623963332678 1103 52-53 -1.1050012725727498 1103 54-55 -0.3833312183168758 1103 56-57 0.4557309162715697 1103 58-59 -0.19217145994297624 1103 60-61 -1.2878357023794962 1103 62-63 -0.5224981206137009 1103 64-65 -0.8486660931389274 1103 66-67 -1.6797716332834618 1103 68-69 -2.058953859483072 1103 70-71 -1.402506143070358 1103 72-73 -0.4502379928582734 1103 74-75 -0.6970202586691592 1103 76-77 -2.0094226196158225 1103 78-79 -1.9732540702460604 1103 80-81 -2.450648684536059 1103 82-83 -1.627746255139133 1103 84-85 -1.3554824262431673 1103 86-87 -1.2437940342564566 1103 88-89 -2.0341742561866845 1103 90-91 -2.821200810102429 1103 92-93 -2.0610057905283696 1103 94-95 -0.43570884189591297 1103 96-97 0.2405165528967288 1103 98-99 0.4132337889621489 1103 100-101 0.49156176795498396 1104 1 0.5688733196295743 1104 2 0.5811631118993148 1104 3 0.1319548515381399 1104 4 0.7092631833287015 1104 5 0.4994815370505208 1104 6 0.5233840930301454 1104 7 0.6761338907871419 1104 8 0.4691256832619075 1104 9 0.9517237841258037 1104 10-11 0.7945941488376462 1104 12-13 0.711658434085642 1104 14-15 1.1107826345098601 1104 16-17 0.8533407608794263 1104 18-19 0.7793043120510106 1104 20-21 0.862316430399872 1104 22-23 0.7712173959706732 1104 24-25 0.7655913071574076 1104 26-27 1.3622515743959696 1104 28-29 1.479521936599646 1104 30-31 1.1961303749783383 1104 32-33 1.2906930571710404 1104 34-35 1.5261927413887193 1104 36-37 1.23995532993586 1104 38-39 1.8082644288850744 1104 40-41 2.2897992333556303 1104 42-43 3.022854622598537 1104 44-45 3.919680784376979 1104 46-47 3.913206803258525 1104 48-49 3.2092023892067942 1104 50-51 3.587285127576415 1104 52-53 3.1290896365181595 1104 54-55 2.748486963501314 1104 56-57 2.8670945526352156 1104 58-59 2.828283085511565 1104 60-61 3.2689824794386837 1104 62-63 4.132047333931755 1104 64-65 2.6808793614065287 1104 66-67 2.8020465485347223 1104 68-69 2.3910461405169308 1104 70-71 2.6224938569296405 1104 72-73 2.0474892798690014 1104 74-75 1.3120706504217523 1104 76-77 1.0792137440205423 1104 78-79 1.308564111572121 1104 80-81 0.6516240427366675 1104 82-83 1.310890108497233 1104 84-85 1.8536084828477364 1104 86-87 1.8630241475617204 1104 88-89 1.9930984710860393 1104 90-91 2.2378900989884762 1104 92-93 1.7071760276534533 1104 94-95 1.0870184308313604 1104 96-97 2.122334734714908 1104 98-99 1.9541428798712381 1104 100-101 2.1415617679549825 1105 1 0.6357182394156737 1105 2 0.7923930584233787 1105 3 0.6346286483295884 1105 4 0.44723109776720804 1105 5 0.6091072054997184 1105 6 0.37899906629218094 1105 7 0.5584868319636129 1105 8 0.5065588383421229 1105 9 0.7752531958905067 1105 10-11 0.4109043092654545 1105 12-13 0.579305492909171 1105 14-15 0.8754885168628022 1105 16-17 0.5806134881521601 1105 18-19 0.5854540446713301 1105 20-21 0.7700704410950578 1105 22-23 1.2070462729760152 1105 24-25 0.2696020023445769 1105 26-27 1.2365831251981092 1105 28-29 1.2134791558509832 1105 30-31 1.5370394658874318 1105 32-33 1.8187679234812038 1105 34-35 2.507476163848608 1105 36-37 2.8803296614866625 1105 38-39 2.0930237871738484 1105 40-41 2.689531853676492 1105 42-43 3.1044054247375783 1105 44-45 3.9571139394571944 1105 46-47 4.423901990424298 1105 48-49 4.945833405249573 1105 50-51 4.231670154314379 1105 52-53 4.705292845074311 1105 54-55 4.228433487565482 1105 56-57 4.171907386859807 1105 58-59 4.915181481233489 1105 60-61 3.866576062326388 1105 62-63 3.603972467621599 1105 64-65 3.7998633186257784 1105 66-67 3.9437577784812454 1105 68-69 2.979281434634583 1105 70-71 2.7548467981061115 1105 72-73 4.03946788949467 1105 74-75 3.3294503295661393 1105 76-77 3.789106792148882 1105 78-79 3.8179224003421766 1105 80-81 3.130233668405115 1105 82-83 3.0501949213314568 1105 84-85 3.268046985521533 1105 86-87 3.3870883186847145 1105 88-89 3.942296332048606 1105 90-91 5.387622719309338 1105 92-93 4.830170680059869 1105 94-95 3.152526452221732 1105 96-97 3.6022812587790796 1105 98-99 3.642645553668032 1105 100-101 3.6268558856020405 1106 1 0.38705513781139445 1106 2 0.3993449300811278 1106 3 0.35922757881086653 1106 4 -0.10891863485311148 1106 5 0.04493608250506753 1106 6 -0.7493431796971279 1106 7 -0.6875024728492249 1106 8 -0.21269249855627237 1106 9 -0.13918530678328978 1106 10-11 -8.603966169005162E-4 1106 12-13 -0.1292506568234515 1106 14-15 0.33805536178258677 1106 16-17 -0.8284774209387464 1106 18-19 -0.629786597039903 1106 20-21 -0.06950175141831494 1106 22-23 0.04394466869793945 1106 24-25 -0.6434996019334989 1106 26-27 -1.0922938801494908 1106 28-29 -1.2250235179458073 1106 30-31 -0.8720514432034747 1106 32-33 -1.9138523973744128 1106 34-35 -2.246534531338561 1106 36-37 -0.5554992155186795 1106 38-39 -1.782644662024019 1106 40-41 -3.6874734939170963 1106 42-43 -4.908963559219643 1106 44-45 -5.625773761077568 1106 46-47 -4.154975014923295 1106 48-49 -2.995343065338659 1106 50-51 -2.7990785087872254 1106 52-53 -2.3254558180272937 1106 54-55 -2.933331218316873 1106 56-57 -2.928359992819331 1106 58-59 -2.1035350963066186 1106 60-61 -1.8673811569249494 1106 62-63 -1.3679526660682448 1106 64-65 -2.750938820411651 1106 66-67 -2.6979534514652848 1106 68-69 -2.5634993140285225 1106 70-71 -1.3093243248885393 1106 72-73 -3.293419811040092 1106 74-75 -2.2333838950327944 1106 76-77 -1.125331710524911 1106 78-79 -1.3277995247915122 1106 80-81 -1.4392850481724224 1106 82-83 -1.098200800593677 1106 84-85 -1.1236642444249867 1106 86-87 0.2493877839253571 1106 88-89 -1.279628801641234 1106 90-91 -1.7621099010115238 1106 92-93 -1.1337330632556402 1106 94-95 -1.344799750986823 1106 96-97 -1.1958470834669086 1106 98-99 -1.0004025746742151 1106 100-101 -0.24480186840865414 1107 1 0.8800621308183878 1107 2 0.8014428321790348 1107 3 0.7613254809087735 1107 4 0.5694030434685615 1107 5 0.1882927258617073 1107 6 0.3835239531700054 1107 7 0.09921081386406172 1107 8 0.20339141752764078 1107 9 0.22095455335657022 1107 10-11 0.4414472956907929 1107 12-13 0.2308892033164085 1107 14-15 0.24539801912524695 1107 16-17 0.15753656507524028 1107 18-19 0.1307029134496105 1107 20-21 0.341337409420845 1107 22-23 0.3970915218447928 1107 24-25 0.4981087896748946 1107 26-27 0.01260122474561598 1107 28-29 0.6263750834527926 1107 30-31 0.9601163889643516 1107 32-33 1.2644692809472673 1107 34-35 0.9667521819481593 1107 36-37 0.24345183343235988 1107 38-39 1.4708518414724807 1107 40-41 1.4768621704185705 1107 42-43 1.641735741479657 1107 44-45 1.9616388263350188 1107 46-47 2.642227782279498 1107 48-49 2.1969646269690344 1107 50-51 2.515606805898095 1107 52-53 2.758460265888793 1107 54-55 2.989745704760047 1107 56-57 2.711500147040802 1107 58-59 2.0520593092877846 1107 60-61 2.491010451466657 1107 62-63 3.081348033232455 1107 64-65 3.168641599168769 1107 66-67 2.8509975974857618 1107 68-69 3.1987384482092374 1107 70-71 3.8340323184681075 1107 72-73 3.9670696994494214 1107 74-75 3.9029797413308422 1107 76-77 3.6963466111534125 1107 78-79 3.6459766989847076 1107 80-81 3.943582084694711 1107 82-83 3.620330667937793 1107 84-85 3.1175944968337532 1107 86-87 3.488898273435847 1107 88-89 3.5735180515056193 1107 90-91 3.021106882205263 1107 92-93 4.02360959408702 1107 94-95 3.0065988504117804 1107 96-97 3.902055014435188 1107 98-99 4.670926096654455 1107 100-101 3.564638691031906 1108 1 0.48974874050499295 1108 2 0.37409240482860895 1108 3 0.2969380165213096 1108 4 0.2446167186822379 1108 5 0.504532042101026 1108 6 0.4661450357910937 1108 7 0.4539116685649205 1108 8 0.3344455485817761 1108 9 0.5443163767183989 1108 10-11 0.036176640420137574 1108 12-13 0.14684361927083245 1108 14-15 0.555227078954303 1108 16-17 0.8213542288929006 1108 18-19 0.41987670262339805 1108 20-21 0.3463231644065985 1108 22-23 0.3856955104487767 1108 24-25 0.5963993879654907 1108 26-27 0.2561909683353605 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88-89 0.7824924104799784 2311 90-91 1.2287991898975683 2311 92-93 1.7389942094716346 2311 94-95 1.4976244914374206 2311 96-97 2.3071832195633952 2311 98-99 1.4799004556288153 2311 100-101 3.2248951012883182 2312 1 -0.47321422245796896 2312 2 -0.4036853729491696 2312 3 -0.036395316812026124 2312 4 0.35572782979335216 2312 5 0.3563838939528736 2312 6 0.4661450357910937 2312 7 0.009467224120477624 2312 8 0.5196307337669595 2312 9 0.3220941544961775 2312 10-11 -0.38974928550578625 2312 12-13 -0.18648971406251036 2312 14-15 0.1293011530283792 2312 16-17 0.32135422889290055 2312 18-19 0.3643211470678409 2312 20-21 -0.46849165040821106 2312 22-23 0.16347328822656237 2312 24-25 0.5963993879654907 2312 26-27 -0.1512164390720443 2312 28-29 -0.4640807570030461 2312 30-31 -0.09259016374220153 2312 32-33 -0.6158725993946206 2312 34-35 -0.11017089497492094 2312 36-37 -0.4410211010405689 2312 38-39 0.49934186996251384 2312 40-41 1.2973749909313952 2312 42-43 1.434470784214696 2312 44-45 1.544259908956107 2312 46-47 1.832398722450442 2312 48-49 2.2268791568835624 2312 50-51 1.329709370000657 2312 52-53 1.2662950237235506 2312 54-55 1.5759280409423866 2312 56-57 1.3788790644197206 2312 58-59 1.2735692807977657 2312 60-61 0.8556828161390229 2312 62-63 1.2608352127196305 2312 64-65 1.698556129083304 2312 66-67 0.8054135519017187 2312 68-69 1.1317868812576712 2312 70-71 1.8169383013740834 2312 72-73 1.6080953404750638 2312 74-75 1.6344612228123232 2312 76-77 2.3729847877915837 2312 78-79 2.5804496334576434 2312 80-81 2.6465735376861623 2312 82-83 2.7898463374534614 2312 84-85 1.777850907090162 2312 86-87 2.273798558336132 2312 88-89 1.7287887067762746 2312 90-91 1.3102806713790507 2312 92-93 1.7038090242864499 2312 94-95 2.138365232178163 2312 96-97 2.9127387751189495 2312 98-99 2.6873078630362244 2312 100-101 2.0397099161031313 2313 1 -0.6928821662638427 2313 2 -1.564604913179057 2313 3 -0.6736877816906954 2313 4 -0.751551863693237 2313 5 -0.756004356366411 2313 6 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0.6256741898975697 2314 92-93 0.5921192094716332 2314 94-95 0.845541158104087 2314 96-97 1.471766552896728 2314 98-99 0.4569837889621482 2314 100-101 1.5321867679549825 2315 1 0.0916005923568477 2315 2 0.3567312937174947 2315 3 0.3791139424472334 2315 4 0.05017227423779502 2315 5 0.2892542643232474 2315 6 0.17679318393923893 2315 7 0.2698838907871419 2315 8 0.4918529559891809 2315 9 0.7017237841258037 2315 10-11 0.5616396033830995 2315 12-13 -0.257091565914358 2315 14-15 -0.014217365490139855 2315 16-17 -0.3568865118478399 2315 18-19 0.1258952211419171 2315 20-21 0.3509527940362318 2315 22-23 -0.2074757858475138 2315 24-25 0.10507994352104788 2315 26-27 -1.5354756983313038 2315 28-29 -0.0034326088549008205 2315 30-31 0.7089144658874318 2315 32-33 0.1827385117164937 2315 34-35 -0.9226708949749209 2315 36-37 -0.6123173973368665 2315 38-39 -0.9630423892967457 2315 40-41 -2.134916675735276 2315 42-43 -1.3294181046741897 2315 44-45 -1.6044669428957476 2315 46-47 -3.379406833105115 2315 48-49 -1.932843065338659 2315 50-51 -2.8473739633326787 2315 52-53 -1.670626272572747 2315 54-55 -1.745831218316873 2315 56-57 -2.1286440837284246 2315 58-59 -2.279671459942982 2315 60-61 -2.037835702379496 2315 62-63 -1.916248120613698 2315 64-65 -1.8674160931389245 2315 66-67 -1.2547716332834646 2315 68-69 -1.1714538594830692 2315 70-71 -3.0337561430703595 2315 72-73 -2.600237992858272 2315 74-75 -2.4095202586691578 2315 76-77 -2.737547619615821 2315 78-79 -2.873254070246059 2315 80-81 -2.544398684536059 2315 82-83 -3.8496212551391302 2315 84-85 -3.41485742624317 2315 86-87 -3.4750440342564595 2315 88-89 -3.4060492561866873 2315 90-91 -4.06182581010243 2315 92-93 -3.204755790528367 2315 94-95 -2.216958841895913 2315 96-97 -2.918858447103272 2315 98-99 -3.574266211037852 2315 100-101 -1.9834382320450175 2316 1 -0.9404506896944334 2316 2 -1.1985571678209652 2316 3 -0.5207258011425111 2316 4 -0.3280328539673363 2316 5 -1.2988867613177817 2316 6 -0.5651939955479435 2316 7 -0.24694303228977788 2316 8 -0.8222496081133812 2316 9 -0.20212236972034958 2316 10-11 -0.257270653027156 2316 12-13 0.2308892033164156 2316 14-15 -1.1584481347209135 2316 16-17 -0.6245147169760443 2316 18-19 -0.0872458044991049 2316 20-21 0.06569638377982301 2316 22-23 0.1919633167165884 2316 24-25 -0.5275322359661345 2316 26-27 -1.051501339356939 2316 28-29 0.19688790396561018 2316 30-31 -0.0014220725741012075 2316 32-33 0.136264152742136 2316 34-35 -0.02042730523132974 2316 36-37 0.4421697821503159 2316 38-39 0.4708518414724807 2316 40-41 -0.266727573171174 2316 42-43 0.36609471583862785 2316 44-45 -0.37169450699830975 2316 46-47 0.18709957715129377 2316 48-49 -0.14918921918481942 2316 50-51 -0.06772652743524077 2316 52-53 0.4315371889657129 2316 54-55 0.43205339706774026 2316 56-57 0.20508989063055338 2316 58-59 -0.3517868445583687 2316 60-61 -0.7397587793025764 2316 62-63 -0.7007032488188258 2316 64-65 -0.4595635290363589 2316 66-67 -0.26438701789885144 2316 68-69 -0.9807487312779379 2316 70-71 -0.25571127127549076 2316 72-73 -0.1483149159351953 2316 74-75 -0.052148463797362155 2316 76-77 0.47839789320468995 2316 78-79 0.33828439129240095 2316 80-81 0.8474282385408607 2316 82-83 0.812638360245483 2316 84-85 -0.5811234518841957 2316 86-87 -0.9213581368205617 2316 88-89 -0.1187896408020741 2316 90-91 -0.6968418357434558 2316 92-93 0.08130190177932661 2316 94-95 0.32070141451434253 2316 96-97 0.5879524503326259 2316 98-99 0.1004132761416372 2316 100-101 0.40438228077549354 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 0.0 9 3.0 10 1.0 11 0.0 12 1.0 13 2.0 14 8.0 15 29.0 16 120.0 17 324.0 18 499.0 19 601.0 20 762.0 21 816.0 22 773.0 23 515.0 24 409.0 25 437.0 26 508.0 27 552.0 28 604.0 29 542.0 30 334.0 31 314.0 32 306.0 33 400.0 34 542.0 35 728.0 36 1449.0 37 3604.0 38 7152.0 39 1914.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.9042904290429 21.959570957095707 12.566006600660065 22.57013201320132 2 12.83479839874541 23.469935206966284 42.03293301968552 21.66233337460278 3 16.766318914683932 34.72434126427776 31.153354500845325 17.355985320192982 4 10.040823058842935 20.246587769576514 38.761288194301265 30.95130097727929 5 8.374912374747433 42.24980413178838 36.89332398663973 12.481959506824461 6 22.881530658529545 42.96317677621541 18.976537050018553 15.178755515236485 7 22.058156320890905 36.40750670241287 23.798721385852755 17.735615590843473 8 25.520597088779844 33.2975959754237 23.796956826522617 17.384850109273845 9 26.926724671147582 13.653045235248031 22.283617170425963 37.136612923178426 10-11 21.147581543029155 29.097769164158176 29.65857078058637 20.0960785122263 12-13 23.972207331656424 27.36588181930642 29.68743556966723 18.974475279369923 14-15 21.65271535194425 27.34320234217146 28.081316234382086 22.922766071502206 16-17 18.793039462290213 31.559523318626038 29.091583852212278 20.555853366871467 18-19 18.316770442455983 28.15760174838151 33.893447692878645 19.632180116283866 20-21 17.943589955053397 27.46897035173807 34.610943878602946 19.97649581460558 22-23 17.399282503814277 30.077110222258874 34.15529256525504 18.368314708671807 24-25 18.727062801533958 31.70590903467898 33.7532472887716 15.813780875015462 26-27 17.595150715434414 31.606944043544594 34.526411282008986 16.271493959012 28-29 16.230258546039337 30.223495938311824 35.63976743227084 17.906478083378005 30-31 19.333223372232073 30.64203537998433 34.6954764751969 15.329264772586699 32-33 15.560183085233598 32.268772421755806 35.334625376273145 16.836419116737456 34-35 16.766318914683932 32.08321306337883 35.59234670735228 15.558121314584966 36-37 16.127170013607685 32.52237021153767 33.6913941693126 17.65906560554204 38-39 15.636468599233023 30.110098552637005 35.65213805616263 18.60129479196734 40-41 15.805533792420931 29.435899550534 36.67271452723599 18.08585212980908 42-43 17.325058760463484 28.429755474001073 35.94903302956579 18.29615273596965 44-45 15.248855717290008 30.219372397014553 35.34699600016494 19.184775885530495 46-47 18.630159581048204 27.5205146179539 32.39454043132242 21.454785369675477 48-49 17.650818522947507 28.029771968166262 32.229598779431775 22.089810729454456 50-51 16.393138427281347 27.871015628221517 33.34089315904499 22.394952785452148 52-53 15.562244855882232 29.25858727475156 35.22122799059832 19.957939878767885 54-55 16.14984949074265 29.29982268772422 32.37804626613335 22.172281555399778 56-57 17.162178879221475 29.967836377881323 30.68945610490289 22.18052863799431 58-59 14.387035586161396 29.765782854315287 34.45424930930683 21.392932250216486 60-61 16.5024122716589 33.30790482866686 29.097769164158176 21.09191373551606 62-63 15.321333580750911 33.55807096760891 30.8199830931321 20.300612358508072 64-65 14.974948970124327 32.93746520690295 30.929259190532154 21.15832663244057 66-67 15.294214671559935 33.24398993855923 29.947218671394992 21.514576718485838 68-69 15.48595934188281 32.173930971918686 30.536885076904046 21.80322460929446 70-71 15.774607232691434 31.244072409385183 30.382252278256566 22.599068079666818 72-73 16.19314667436394 31.435817079708052 29.05653375118552 23.314502494742484 74-75 16.362211867551853 30.839965362253103 28.852418456970845 23.9454043132242 76-77 16.37458249144365 30.69770318749742 29.38435528431817 23.543359036740753 78-79 16.242629169931135 29.96371283658406 29.250340192157026 24.54331780132778 80-81 16.66529215290091 30.782235784091377 27.815347820708425 24.737124242299284 82-83 17.640509669704343 30.04206012123211 27.47309389303534 24.844336316028205 84-85 16.545709455280193 30.52657622366088 26.50612345882644 26.421590862232485 86-87 17.838439651973115 30.18638406663643 26.996824873201103 24.978351408189354 88-89 18.205434827429798 30.355449259824336 26.328811183044 25.110304729701866 90-91 18.03843140489052 30.03381303863758 26.44427033936745 25.48348521710445 92-93 18.520885736670653 30.753370995010513 25.792750814399408 24.932992453919425 94-95 18.283782112077855 29.79258587274752 26.326749412395365 25.59688260277927 96-97 18.747680508020288 29.967836377881323 25.80924497958847 25.475238134509915 98-99 18.18106843157873 29.62000783489 26.15409991546566 26.044823818065606 100-101 18.346391752577322 30.107216494845364 26.22680412371134 25.319587628865982 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1.0 1 2.5 2 4.5 3 5.0 4 3.0 5 4.0 6 10.5 7 19.5 8 32.0 9 33.5 10 27.5 11 29.5 12 31.0 13 33.0 14 31.0 15 26.0 16 31.0 17 36.5 18 46.0 19 52.5 20 50.5 21 51.5 22 60.0 23 83.5 24 115.0 25 145.0 26 179.0 27 237.0 28 278.5 29 348.0 30 448.0 31 495.0 32 593.5 33 755.5 34 937.0 35 1075.5 36 1207.0 37 1368.0 38 1419.0 39 1439.5 40 1380.5 41 1262.5 42 1163.5 43 1185.0 44 1259.5 45 1252.0 46 1116.0 47 852.0 48 701.5 49 632.5 50 518.5 51 356.5 52 228.5 53 151.0 54 111.0 55 79.0 56 58.0 57 53.0 58 43.0 59 30.5 60 20.0 61 13.5 62 7.5 63 6.5 64 4.0 65 4.0 66 4.5 67 2.0 68 1.5 69 2.0 70 1.0 71 0.5 72 0.5 73 1.0 74 0.5 75 0.5 76 0.5 77 0.0 78 0.5 79 1.0 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04535895426992701 2 0.08247082594532185 3 0.0 4 0.0 5 0.0 6 0.0 7 0.024741247783596553 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0020617706486330462 64-65 0.0020617706486330462 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0020617706486330462 100-101 0.0041235412972660925 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 24251.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.80833780050307 #Duplication Level Percentage of deduplicated Percentage of total 1 75.04743833017078 35.878932827512266 2 10.117302052785924 9.673827883386252 3 4.605830602035535 6.60591315822028 4 2.647921338623426 5.063708713042761 5 1.906158357771261 4.556513133479031 6 1.3282732447817838 3.810152158673869 7 0.8366396411937208 2.7998845408436766 8 0.724512678971882 2.771019751762814 9 0.53475935828877 2.3009360438744793 >10 2.1735380369156463 17.265267411653127 >50 0.017250301880282905 0.6350253597789782 >100 0.051750905640848714 5.641004494660014 >500 0.008625150940141452 2.997814523112449 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 727 2.997814523112449 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 319 1.3154096738278835 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 274 1.1298503154509092 RNA PCR Primer, Index 17 (95% over 21bp) ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT 231 0.9525380396684673 RNA PCR Primer, Index 17 (95% over 23bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 199 0.8205847181559524 No Hit TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG 176 0.7257432683188322 RNA PCR Primer, Index 17 (96% over 28bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 169 0.6968784792379695 No Hit CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT 92 0.37936579934848047 RNA PCR Primer, Index 17 (96% over 26bp) CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG 62 0.2556595604304977 TruSeq Adapter, Index 12 (95% over 23bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 49 0.20205352356603853 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 48 0.19792998226877243 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 48 0.19792998226877243 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 47 0.19380644097150632 No Hit CCCTAGATGACACATGAGCAAAAGCCCACTTCGCCATCATATTCGTAGGA 46 0.18968289967424024 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 46 0.18968289967424024 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 45 0.18555935837697415 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 40 0.1649416518906437 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 38 0.1566945692961115 No Hit GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG 37 0.15257102799884542 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 36 0.1484474867015793 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 36 0.1484474867015793 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36 0.1484474867015793 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 35 0.14432394540431323 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 35 0.14432394540431323 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 34 0.14020040410704712 No Hit CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA 33 0.13607686280978104 RNA PCR Primer, Index 17 (96% over 28bp) CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC 32 0.13195332151251496 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 30 0.12370623891798276 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 29 0.11958269762071666 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 29 0.11958269762071666 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 29 0.11958269762071666 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 28 0.11545915632345058 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 28 0.11545915632345058 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 27 0.11133561502618448 No Hit GCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAAT 27 0.11133561502618448 No Hit TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT 27 0.11133561502618448 No Hit TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC 27 0.11133561502618448 No Hit GTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGG 27 0.11133561502618448 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 27 0.11133561502618448 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26 0.10721207372891839 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 25 0.10308853243165231 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.006185311945899139 0.0 18-19 0.0 0.0 0.0 0.012370623891798277 0.0 20-21 0.0 0.0 0.0 0.012370623891798277 0.0 22-23 0.0 0.0 0.0 0.02061770648633046 0.0 24-25 0.0 0.0 0.0 0.026803018432229597 0.0 26-27 0.0 0.0 0.0 0.04123541297266092 0.0 28-29 0.0 0.0 0.0 0.05566780751309224 0.0 30-31 0.0 0.0 0.0 0.12370623891798277 0.0 32-33 0.0 0.0 0.0 0.270091954970929 0.0 34-35 0.0 0.0 0.0 0.447404230753371 0.0 36-37 0.0 0.0 0.0 0.6845078553461713 0.0 38-39 0.0 0.0 0.0 1.0205764710733578 0.0 40-41 0.0 0.0 0.0 1.4597336192321966 0.0 42-43 0.0 0.0 0.0 1.8659024370129067 0.0 44-45 0.0 0.0 0.0 2.214341676631891 0.0 46-47 0.0 0.0 0.0 2.6266958063585006 0.0 48-49 0.0 0.0 0.0 2.997814523112449 0.0 50-51 0.0 0.0 0.0 3.410168652839058 0.0 52-53 0.0 0.0 0.0 3.8287080945115664 0.0 54-55 0.0 0.0 0.0 4.216320976454579 0.0 56-57 0.0 0.0 0.0 4.643107500721619 0.0 58-59 0.0 0.0 0.0 5.117314749907221 0.0 60-61 0.0 0.0 0.0 5.591521999092821 0.0 62-63 0.0 0.0 0.0 6.117273514494247 0.0 64-65 0.0 0.0 0.0 6.59972784627438 0.0 66-67 0.0 0.0 0.0 7.111046967135376 0.0 68-69 0.0 0.0 0.0 7.632674941239537 0.0 70-71 0.0 0.0 0.0 8.269762071667149 0.0 72-73 0.0 0.0 0.0 8.865613789122097 0.0 74-75 0.0 0.0 0.0 9.418168322955754 0.0 76-77 0.0 0.0 0.0 10.001649416518907 0.0 78-79 0.0 0.0 0.0 10.67378664797328 0.0 80-81 0.0 0.0 0.0 11.312935549049524 0.0 82-83 0.0 0.0 0.0 11.952084450125767 0.0 84-85 0.0 0.0 0.0 12.667518865201435 0.0 86-87 0.0 0.0 0.0 13.337594326007174 0.0 88-89 0.0 0.0 0.0 13.995299162921118 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCAAGC 15 6.5394E-4 94.99999 7 CACCAAG 20 0.0020496037 71.25 6 ATCACCA 20 0.0020496037 71.25 4 CTATCAC 20 0.0020496037 71.25 2 AGATGGA 25 0.0049623605 57.0 6 TATCACC 25 0.0049623605 57.0 3 AGTAATG 45 9.43959E-4 42.222225 3 TGGGAAG 50 0.001584815 38.0 6 GGTATCA 165 0.0 37.424244 1 CATGGGA 115 2.2937456E-9 37.173912 4 GTACATG 220 0.0 36.704544 1 AGTACAT 140 4.0927262E-10 33.928574 2 TACATGG 240 0.0 33.64583 2 GTATCAA 270 0.0 33.425926 1 ACATGGG 235 0.0 32.340424 3 GAGTACA 165 6.730261E-11 31.666666 1 AACGCAG 290 0.0 29.482758 6 ATCAACG 325 0.0 27.76923 3 TCAACGC 320 0.0 26.718752 4 TATCAAC 340 0.0 26.544119 2 >>END_MODULE