FastQCFastQC Report
Fri 10 Feb 2017
SRR2568632_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568632_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4095668
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42750.032.4457361
TCAACGC39650.032.344444
CAACGCA42700.030.1453635
ATCAACG42800.029.9639533
GGTATCA24200.029.0507431
AACGCAG46650.027.6950196
GTACATG93700.024.7902071
ACATGGG86700.024.1053093
CATGGGG49450.024.0133534
TATCAAC54950.023.6861132
TACATGG95100.023.5761242
CGCAGAG54700.023.1850668
ACGCAGA56800.022.4951237
AGAGTAC60050.017.71797410-11
GACCCGT3007.695962E-817.4162986
GTATTAT23750.017.2006861
GAGTACA64150.016.8089311
GTATTAC13450.016.5991741
TATAAGA29750.016.1266922
GCAGAGT78850.016.0237589
Produced by FastQC (version 0.11.5)