##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568632_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4095668 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06095928673906 34.0 31.0 34.0 30.0 34.0 2 32.2075722446253 34.0 31.0 34.0 30.0 34.0 3 32.24726955407518 34.0 31.0 34.0 30.0 34.0 4 35.59581562763388 37.0 35.0 37.0 33.0 37.0 5 35.63952522518915 37.0 35.0 37.0 33.0 37.0 6 35.75045584749545 37.0 36.0 37.0 35.0 37.0 7 35.733670795582064 37.0 36.0 37.0 35.0 37.0 8 35.75298022202971 37.0 36.0 37.0 35.0 37.0 9 37.5493247987874 39.0 38.0 39.0 35.0 39.0 10-11 37.52398631920361 39.0 38.0 39.0 35.0 39.0 12-13 37.50110934284713 39.0 38.0 39.0 35.0 39.0 14-15 38.921907122354646 41.0 39.0 41.0 36.0 41.0 16-17 38.89856416584547 41.0 39.0 41.0 35.5 41.0 18-19 38.88406323461765 41.0 39.0 41.0 35.0 41.0 20-21 38.89131162486803 41.0 39.0 41.0 35.0 41.0 22-23 38.829268265884835 41.0 39.0 41.0 35.0 41.0 24-25 38.78052151688076 41.0 39.0 41.0 35.0 41.0 26-27 38.703595970181176 41.0 39.0 41.0 34.0 41.0 28-29 38.593110818552674 40.5 39.0 41.0 34.0 41.0 30-31 38.526962512586465 40.0 38.0 41.0 34.0 41.0 32-33 38.44288465275994 40.0 38.0 41.0 34.0 41.0 34-35 38.316568261880604 40.0 38.0 41.0 34.0 41.0 36-37 38.229906818619085 40.0 38.0 41.0 33.0 41.0 38-39 38.15101468185409 40.0 38.0 41.0 33.0 41.0 40-41 38.04800657670495 40.0 38.0 41.0 33.0 41.0 42-43 37.91878675224652 40.0 38.0 41.0 33.0 41.0 44-45 37.76910262257585 40.0 38.0 41.0 32.0 41.0 46-47 37.854522998446164 40.0 38.0 41.0 32.5 41.0 48-49 38.04821911834651 40.0 38.0 41.0 33.0 41.0 50-51 36.98588984751694 39.0 36.5 40.0 31.5 40.5 52-53 37.14377483721825 39.5 37.0 40.0 31.5 40.5 54-55 37.780685104359044 40.0 38.0 41.0 32.5 41.0 56-57 37.79012739802152 40.0 37.5 41.0 32.0 41.0 58-59 37.67642238091564 40.0 37.0 41.0 32.0 41.0 60-61 37.50708626773459 40.0 37.0 41.0 32.0 41.0 62-63 37.28758983882483 40.0 36.0 41.0 31.0 41.0 64-65 37.163279469917974 39.0 36.0 41.0 31.0 41.0 66-67 37.082845338049864 39.0 36.0 41.0 31.0 41.0 68-69 36.830308999655244 39.0 35.0 41.0 31.0 41.0 70-71 36.49889602868201 38.5 35.0 41.0 31.0 41.0 72-73 36.16233591199287 37.5 35.0 40.0 30.5 41.0 74-75 35.80824397876 37.0 35.0 39.5 30.0 41.0 76-77 35.42834465586566 36.5 35.0 39.0 30.0 41.0 78-79 35.030220955409476 36.0 35.0 39.0 30.0 40.0 80-81 34.618408767507525 36.0 34.0 37.5 29.0 39.0 82-83 34.24937568181796 35.0 34.0 37.0 29.0 39.0 84-85 33.89403096149395 35.0 34.0 37.0 29.0 38.5 86-87 33.60143961864097 35.0 34.0 36.0 29.0 37.0 88-89 33.350316846971 35.0 34.0 36.0 29.0 37.0 90-91 33.13587649194222 35.0 34.0 36.0 28.0 36.5 92-93 32.95497291284352 35.0 34.0 35.0 27.5 36.0 94-95 32.7922829682484 35.0 34.0 35.0 27.0 36.0 96-97 32.679303840057344 35.0 34.0 35.0 27.0 36.0 98-99 32.54068408376851 35.0 33.0 35.0 26.5 36.0 100-101 31.275576169748135 34.5 31.0 35.0 22.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32936152576128563 1101 2 0.31745543150414335 1101 3 0.4340403276077893 1101 4 0.3307688404271758 1101 5 0.19739129740997186 1101 6 0.21016308405994266 1101 7 0.3005855116249805 1101 8 0.2611462527609305 1101 9 0.3298544744054155 1101 10-11 0.2992460783351518 1101 12-13 0.35285150383158026 1101 14-15 0.42980036599685434 1101 16-17 0.4249733339746484 1101 18-19 0.4368629404516966 1101 20-21 0.30830478389689375 1101 22-23 0.2401311283259986 1101 24-25 0.3043757312231179 1101 26-27 0.39178838086912293 1101 28-29 0.39976406762239947 1101 30-31 0.33678807154958434 1101 32-33 0.3251573360455353 1101 34-35 0.2737936242627512 1101 36-37 0.31479727957074743 1101 38-39 0.36384148883937684 1101 40-41 0.39228789583503243 1101 42-43 0.41802944013833354 1101 44-45 0.46380586472508156 1101 46-47 0.39487994989804776 1101 48-49 0.44717608405235865 1101 50-51 0.24827961766570894 1101 52-53 0.3539988007853907 1101 54-55 0.37941867659635875 1101 56-57 0.4169057041319135 1101 58-59 0.4213336353344843 1101 60-61 0.44941280568636444 1101 62-63 0.4511324283571625 1101 64-65 0.4655764725411302 1101 66-67 0.4238424869617461 1101 68-69 0.4047578643284524 1101 70-71 0.4489178030778618 1101 72-73 0.37551513190483377 1101 74-75 0.3361107711762443 1101 76-77 0.3121353677311802 1101 78-79 0.23521308156261966 1101 80-81 0.19385069153155854 1101 82-83 0.24910510216194126 1101 84-85 0.29199825234596943 1101 86-87 0.29855397817475904 1101 88-89 0.3422627273222574 1101 90-91 0.26492566274796303 1101 92-93 0.21874018258821337 1101 94-95 0.21427293087445065 1101 96-97 0.18527553252479834 1101 98-99 0.21384364803869005 1101 100-101 0.036425034028152226 1102 1 0.2580850303924578 1102 2 0.15216212959861508 1102 3 0.32948667406729726 1102 4 0.281333387697309 1102 5 0.3310153787029151 1102 6 0.3220165964781785 1102 7 0.3352155039777074 1102 8 0.3416558304949504 1102 9 0.4220640074672204 1102 10-11 0.3599987585223019 1102 12-13 0.35733656193749397 1102 14-15 0.44914468624827464 1102 16-17 0.3407855790532821 1102 18-19 0.37496615364764807 1102 20-21 0.3430482040643881 1102 22-23 0.310064253965578 1102 24-25 0.3143557772465684 1102 26-27 0.40695283518378034 1102 28-29 0.5035240176733922 1102 30-31 0.41561319527180984 1102 32-33 0.3962086502647537 1102 34-35 0.38358643430800043 1102 36-37 0.31694825825330497 1102 38-39 0.3134560235581887 1102 40-41 0.2728482612735377 1102 42-43 0.22602403346103728 1102 44-45 0.24293817543167506 1102 46-47 0.2989155047857466 1102 48-49 0.24908474364718813 1102 50-51 0.3374663504262756 1102 52-53 0.16862003924821778 1102 54-55 0.16762164511438016 1102 56-57 0.2882551194617591 1102 58-59 0.1829438239770198 1102 60-61 0.1694257821778251 1102 62-63 0.3147508426275749 1102 64-65 0.2540057142608987 1102 66-67 0.19604791099539653 1102 68-69 0.1286859205164319 1102 70-71 0.15533531295437797 1102 72-73 0.1169528607625594 1102 74-75 0.1325840688999378 1102 76-77 0.022748250684735183 1102 78-79 0.0770630382591122 1102 80-81 0.054737592206066665 1102 82-83 0.09355188602473419 1102 84-85 0.1030591824482272 1102 86-87 0.10035590787455817 1102 88-89 0.11856742384533447 1102 90-91 0.14550765081867922 1102 92-93 0.06299731339742465 1102 94-95 -0.031228833590581928 1102 96-97 0.03588149041177502 1102 98-99 0.0014962950856940438 1102 100-101 -0.25819133825038065 1103 1 0.23530219357877513 1103 2 0.22568370628192014 1103 3 0.31294445360547485 1103 4 0.29162431913336206 1103 5 0.23004827895186963 1103 6 0.1977572694446934 1103 7 0.2666296502323533 1103 8 0.257124224466736 1103 9 0.3345805236067818 1103 10-11 0.3260360837607905 1103 12-13 0.3896429578659806 1103 14-15 0.39071617881536014 1103 16-17 0.43617910457108167 1103 18-19 0.34998506407083596 1103 20-21 0.3238806471836355 1103 22-23 0.3679922412646732 1103 24-25 0.35457762184932307 1103 26-27 0.30138379860392916 1103 28-29 0.29519147948960267 1103 30-31 0.3556231201517406 1103 32-33 0.27693197766237887 1103 34-35 0.29106131359016274 1103 36-37 0.2754171380791419 1103 38-39 0.20583843436961757 1103 40-41 0.204524524158316 1103 42-43 0.23099431889922073 1103 44-45 0.31577648894563737 1103 46-47 0.26432858462116826 1103 48-49 0.29793187575835134 1103 50-51 0.31460074737992016 1103 52-53 0.2537898146315527 1103 54-55 0.2823511578566382 1103 56-57 0.3067334760565714 1103 58-59 0.2910655379773459 1103 60-61 0.32850951404011397 1103 62-63 0.30707330269728317 1103 64-65 0.26714299834952016 1103 66-67 0.11819743416718609 1103 68-69 0.20912826680270769 1103 70-71 0.17908049275783355 1103 72-73 0.15350934868102684 1103 74-75 0.13132967631715076 1103 76-77 0.11362942622251637 1103 78-79 0.123137784295416 1103 80-81 0.1805262568651429 1103 82-83 0.13048668842689182 1103 84-85 0.12365645355152566 1103 86-87 0.0014181004432387567 1103 88-89 -0.03736087190110737 1103 90-91 -0.010888228200414574 1103 92-93 -0.04128365159889569 1103 94-95 0.04247788924686802 1103 96-97 0.08981796835305289 1103 98-99 0.07258393668062268 1103 100-101 -0.04645725633146114 1104 1 0.2661973033260665 1104 2 0.2583946923065028 1104 3 0.30901054805955397 1104 4 0.344395130643413 1104 5 0.3346043749254477 1104 6 0.1425338685224986 1104 7 0.2795506238454024 1104 8 0.27704991661492784 1104 9 0.3247642492997329 1104 10-11 0.3272236176303025 1104 12-13 0.2569369808306945 1104 14-15 0.40662536752045497 1104 16-17 0.31922518861721016 1104 18-19 0.31820141341186314 1104 20-21 0.3022501169569054 1104 22-23 0.37489341827630085 1104 24-25 0.41299974988312727 1104 26-27 0.40077515597951674 1104 28-29 0.49974495330771873 1104 30-31 0.45951886997389835 1104 32-33 0.37328260960184423 1104 34-35 0.3024572012435769 1104 36-37 0.3069090235164964 1104 38-39 0.27305957485933163 1104 40-41 0.3351813875493903 1104 42-43 0.28434960213908056 1104 44-45 0.36764756495721684 1104 46-47 0.30669038021944317 1104 48-49 0.38025797642492165 1104 50-51 0.32240922380001535 1104 52-53 0.33373277938844126 1104 54-55 0.42630345883167564 1104 56-57 0.41942934770811036 1104 58-59 0.35119905947835406 1104 60-61 0.3151610618514269 1104 62-63 0.32934958913347856 1104 64-65 0.2977461411709328 1104 66-67 0.19664724947331536 1104 68-69 0.22946311605566905 1104 70-71 0.2229220580905178 1104 72-73 0.1588452333492114 1104 74-75 0.18201698360552854 1104 76-77 0.17551538800896793 1104 78-79 0.20623093267042236 1104 80-81 0.1775172326162604 1104 82-83 0.12990241840135042 1104 84-85 0.1639166923962776 1104 86-87 0.17408031437616245 1104 88-89 0.1283283919186644 1104 90-91 0.19135203385376798 1104 92-93 0.12955666854611536 1104 94-95 0.17915854970731715 1104 96-97 0.1416026477037846 1104 98-99 0.1454790854240784 1104 100-101 0.06407278214026846 1105 1 0.20417516006623515 1105 2 0.14076249764779902 1105 3 0.30045865355993584 1105 4 0.08914471355655706 1105 5 0.13488683202969298 1105 6 0.11493426617160907 1105 7 0.132215102389992 1105 8 0.23147519745819523 1105 9 0.2231499832793702 1105 10-11 0.25270332131572815 1105 12-13 0.2149822385207969 1105 14-15 0.35766743914033583 1105 16-17 0.3570394382840476 1105 18-19 0.3390682009608028 1105 20-21 0.4049627595303136 1105 22-23 0.3614514582621382 1105 24-25 0.3810097614023249 1105 26-27 0.39379646434836246 1105 28-29 0.4765736442933317 1105 30-31 0.38658988735024735 1105 32-33 0.32530994582621986 1105 34-35 0.46678110451671984 1105 36-37 0.4651034354849841 1105 38-39 0.39872555104341956 1105 40-41 0.46104145436936506 1105 42-43 0.4046052480534925 1105 44-45 0.47701268917953144 1105 46-47 0.45980020568132574 1105 48-49 0.5287593686271492 1105 50-51 0.5580003610449111 1105 52-53 0.5008954913209251 1105 54-55 0.5166933184925284 1105 56-57 0.5115603567355507 1105 58-59 0.4655813836025402 1105 60-61 0.5161772045116209 1105 62-63 0.5576823032320917 1105 64-65 0.486739817804164 1105 66-67 0.5005629527398554 1105 68-69 0.5154767913404612 1105 70-71 0.4369523149988055 1105 72-73 0.4470814472477116 1105 74-75 0.43972541586101244 1105 76-77 0.46271688220561913 1105 78-79 0.37947913198844674 1105 80-81 0.48692367103156897 1105 82-83 0.3787020832973411 1105 84-85 0.35963271183116063 1105 86-87 0.39092459860059847 1105 88-89 0.3612442917145344 1105 90-91 0.3366834583183689 1105 92-93 0.3976199725052538 1105 94-95 0.34995809410357737 1105 96-97 0.38859173243660905 1105 98-99 0.3584744433154512 1105 100-101 0.3195660635630837 1106 1 0.1460942781600778 1106 2 0.16350584041652638 1106 3 0.2087640848196557 1106 4 0.23097108269281819 1106 5 0.1910124191626963 1106 6 0.1912932275667174 1106 7 0.13240147448414064 1106 8 0.15975031620322966 1106 9 0.29973952729613984 1106 10-11 0.19691973879839253 1106 12-13 0.18521264630665968 1106 14-15 0.26804668091473616 1106 16-17 0.28002391114551983 1106 18-19 0.3017378203461405 1106 20-21 0.3338638835811878 1106 22-23 0.2990614471758235 1106 24-25 0.3153408983967836 1106 26-27 0.2359385482420393 1106 28-29 0.30795429822507714 1106 30-31 0.18763094137508318 1106 32-33 0.1528956597925628 1106 34-35 0.2697624776254841 1106 36-37 0.21756836944291535 1106 38-39 0.17267121236412208 1106 40-41 0.26193475781437314 1106 42-43 0.2911024930999062 1106 44-45 0.30181930215727704 1106 46-47 0.1893689588555958 1106 48-49 0.19162908901598286 1106 50-51 0.1262508465725105 1106 52-53 0.19525550345051812 1106 54-55 0.31910077876467824 1106 56-57 0.29854568743889587 1106 58-59 0.2284853402799527 1106 60-61 0.22276572674213924 1106 62-63 0.278336365384213 1106 64-65 0.1851454872991667 1106 66-67 0.18915345520073856 1106 68-69 0.22118149711759827 1106 70-71 0.30521454461954534 1106 72-73 0.20339251796505664 1106 74-75 0.16502842636148074 1106 76-77 0.23497294184239337 1106 78-79 0.24158642499146765 1106 80-81 0.22071628510762054 1106 82-83 0.2095463710965575 1106 84-85 0.13757501917070414 1106 86-87 0.19383521336911969 1106 88-89 0.19281031458420017 1106 90-91 0.22473720771504446 1106 92-93 0.16595351220215093 1106 94-95 0.13722549083868074 1106 96-97 0.27289073977053846 1106 98-99 0.1963287150985522 1106 100-101 0.05967680627701455 1107 1 0.16338852504510015 1107 2 0.11699196571922954 1107 3 0.2776058545079181 1107 4 0.14128475115978745 1107 5 0.2478179263732727 1107 6 0.15862621281619482 1107 7 0.19989254781754795 1107 8 0.21016142007819383 1107 9 0.21957263418782702 1107 10-11 0.20858639610796814 1107 12-13 0.2483208573427973 1107 14-15 0.24373999471045948 1107 16-17 0.28814984451553016 1107 18-19 0.2560871374654994 1107 20-21 0.20789811463401264 1107 22-23 0.25421851533663187 1107 24-25 0.3487678260514784 1107 26-27 0.287004063799138 1107 28-29 0.3877156783214133 1107 30-31 0.3277114170522566 1107 32-33 0.1128712798871021 1107 34-35 0.216727761875255 1107 36-37 0.18230815336892903 1107 38-39 0.20318901984754945 1107 40-41 0.11033593474951431 1107 42-43 0.19161296930107596 1107 44-45 0.4073110501370749 1107 46-47 0.2823739003363386 1107 48-49 0.29199981703524003 1107 50-51 0.15928992272802844 1107 52-53 0.2256035966621326 1107 54-55 0.2518050347447911 1107 56-57 0.2515963570324615 1107 58-59 0.2922443371247354 1107 60-61 0.28847532655799313 1107 62-63 0.3287761253560646 1107 64-65 0.40460112906473 1107 66-67 0.3033081167172682 1107 68-69 0.27372461460151953 1107 70-71 0.29749213587927414 1107 72-73 0.31102551968302805 1107 74-75 0.27888833945279856 1107 76-77 0.29167423443120555 1107 78-79 0.24054825553825765 1107 80-81 0.2599244925880626 1107 82-83 0.24931471754853618 1107 84-85 0.28606775525951633 1107 86-87 0.36032783810852465 1107 88-89 0.24677816642024908 1107 90-91 0.3169367374275609 1107 92-93 0.24020251192892061 1107 94-95 0.25609659501306936 1107 96-97 0.20614998832112974 1107 98-99 0.29555460280595014 1107 100-101 0.2714217356683939 1108 1 0.18035388205584724 1108 2 0.07907358486645677 1108 3 0.21342122917950235 1108 4 0.20524999822060863 1108 5 0.17419127292563275 1108 6 0.06604508927816966 1108 7 0.1865375958669162 1108 8 0.13508533660721156 1108 9 0.1520999427659504 1108 10-11 0.023768942193576947 1108 12-13 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pass #Quality Count 5 1.0 6 28.0 7 411.0 8 1025.0 9 1150.0 10 1415.0 11 2187.0 12 3664.0 13 5067.0 14 6138.0 15 7183.0 16 8058.0 17 9289.0 18 10269.0 19 12002.0 20 13872.0 21 15379.0 22 17044.0 23 18738.0 24 20311.0 25 22468.0 26 25635.0 27 29625.0 28 34371.0 29 41009.0 30 49122.0 31 60816.0 32 76773.0 33 101303.0 34 142025.0 35 213827.0 36 369723.0 37 765832.0 38 1395362.0 39 614361.0 40 185.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.40426168848658 21.11258945665877 13.511291518645589 23.97185733620907 2 13.650146532901166 21.496261836054078 42.91743807452691 21.936153556517844 3 16.557163105328762 28.457529473667503 31.084247626419053 23.901059794584683 4 10.789124661958583 18.28907827129844 42.88157294613898 28.040224120603995 5 11.632250882847812 39.49911037654744 37.49664575211211 11.371992988492641 6 27.48425036216659 41.22743768695866 19.943542788886607 11.344769161988147 7 24.762531003676735 33.37587614839963 25.959020208122023 15.902572639801615 8 23.123953886141305 38.43584994805293 22.432221588737924 16.00797457706784 9 25.19256421920566 16.29118322639594 21.926816684235213 36.589435870163186 10-11 22.535138358909684 27.58695542029598 31.70123680918978 18.176669411604557 12-13 23.426215412473127 25.045186420767894 31.267293992345564 20.261304174413418 14-15 20.912608150855977 26.847085750114513 27.894436267783423 24.345869831246088 16-17 21.012933665521714 29.701613998009602 28.442429903986362 20.843022432482318 18-19 20.85545633404319 29.46799565003167 29.371393440181343 20.3051545757438 20-21 21.650172599273272 28.713899796221032 29.03606150384289 20.599866100662805 22-23 21.31131570449922 28.66321219486737 28.694989754392303 21.330482346241112 24-25 21.191484208291573 28.809547382938504 28.779576300455034 21.21939210831488 26-27 21.243848325913252 28.891060903816758 28.69691027892228 21.168180491347705 28-29 21.383532164822167 28.678699960823366 28.722405897029546 21.21536197732492 30-31 21.538061263272816 28.530707272640477 28.777309472085953 21.15392199200075 32-33 21.22096096566822 28.613680706681304 28.839639900958357 21.325718426692116 34-35 21.350192447239376 28.852399657394106 28.718196885099083 21.079211010267436 36-37 21.48770883961025 28.6992204200195 28.603131065098797 21.209939675271453 38-39 21.31997040769986 28.776607433234204 28.695338480244942 21.208083678820998 40-41 21.48236868808702 28.530486357780955 28.710566872119518 21.276578082012506 42-43 21.32820464802163 28.554518671182507 28.65386782314386 21.463408857652002 44-45 21.423405650161698 28.7475690582779 28.568097498922633 21.26092779263777 46-47 21.363443360695506 28.615521649436204 28.579299470133122 21.44173551973517 48-49 21.377549139321737 28.587803491058217 28.62289036219564 21.411757007424402 50-51 21.38586734581137 28.61086690936136 28.529058475389164 21.474207269438107 52-53 21.369786320570906 28.57481368118705 28.582773310727333 21.472626687514712 54-55 21.446367234844228 28.478712141706797 28.624036420920834 21.450884202528133 56-57 21.321357102186994 28.579831177722415 28.614819365241516 21.483992354849075 58-59 21.414540924703857 28.511331973197045 28.59349195296103 21.480635149138063 60-61 21.560707557350838 28.55036101559013 28.575106673685465 21.313824753373563 62-63 21.34361844962932 28.520712457457677 28.659677748974698 21.475991343938304 64-65 21.397325108507538 28.579491294003667 28.574205091943288 21.448978505545508 66-67 21.390233641448315 28.544537008347355 28.511379060536207 21.553850289668127 68-69 21.44151810459128 28.475009583993632 28.535614608692256 21.54785770272283 70-71 21.44051617780163 28.338968997053698 28.57574500511885 21.644769820025818 72-73 21.39307922419493 28.411201786863582 28.54343906781507 21.652279921126418 74-75 21.37308248617808 28.361698262652148 28.53209781652224 21.733121434647533 76-77 21.248780418725346 28.361551766402943 28.61539314221758 21.77427467265413 78-79 21.273928941506 28.260847803093412 28.65151667566805 21.813706579732536 80-81 21.365624458951366 28.387431481417718 28.512460266120836 21.73448379351008 82-83 21.380844013810783 28.402384202697522 28.580092012476545 21.636679771015153 84-85 21.435179696303656 28.260197214710654 28.590113476786268 21.71450961219943 86-87 21.136117747480903 28.275448872222775 28.803046884319517 21.78538649597681 88-89 21.28337838728819 28.130895780068325 28.72402071749339 21.861705115150095 90-91 21.41705078126908 28.218663036991238 28.55712587530756 21.807160306432124 92-93 21.459257388093658 27.992350233689272 28.633892337499546 21.91450004071752 94-95 21.276828017388755 27.951362770478188 28.779526693694134 21.99228251843892 96-97 21.258318662826266 27.82904129177462 28.951699835503923 21.960940209895185 98-99 21.243731703031774 27.8333691280445 28.66024527048897 22.262653898434756 100-101 21.39430305908841 27.75069183418934 28.71943674043993 22.135568366282325 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13.0 1 12.0 2 27.0 3 52.5 4 49.5 5 49.0 6 73.0 7 124.5 8 200.0 9 236.5 10 241.5 11 238.5 12 253.0 13 312.0 14 367.0 15 386.0 16 431.5 17 503.5 18 553.5 19 607.5 20 742.0 21 918.5 22 1116.5 23 1377.0 24 1740.5 25 2419.0 26 3791.0 27 6359.5 28 11207.0 29 20671.0 30 36973.5 31 59580.5 32 87463.5 33 121189.0 34 155922.5 35 183354.5 36 209367.0 37 226872.0 38 226488.0 39 218789.0 40 206879.5 41 191204.0 42 174198.5 43 161080.5 44 151265.0 45 142389.0 46 135948.5 47 131908.5 48 127854.0 49 122488.5 50 116635.5 51 109435.0 52 102439.0 53 95105.5 54 86839.5 55 79017.5 56 70489.0 57 60965.0 58 53017.0 59 45016.5 60 36192.0 61 28500.5 62 22140.5 63 16613.5 64 11866.0 65 8649.5 66 6321.0 67 4783.5 68 3730.0 69 2867.5 70 2226.0 71 1717.5 72 1304.0 73 992.5 74 736.0 75 542.5 76 369.0 77 230.5 78 166.0 79 119.5 80 85.0 81 65.0 82 45.0 83 27.0 84 22.5 85 22.0 86 18.5 87 14.5 88 12.0 89 10.0 90 8.0 91 7.5 92 6.0 93 3.5 94 2.5 95 1.5 96 3.5 97 3.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006079594342119528 2 0.008350286204838868 3 0.0015382106166808443 4 3.906566645538652E-4 5 0.002807844776480906 6 0.004321639351627134 7 0.0045413837254386835 8 0.0037356543547963357 9 0.006860907671227258 10-11 0.006116218404421452 12-13 0.0032839575864059296 14-15 0.0 16-17 0.0 18-19 1.4649624920769945E-4 20-21 0.0013184662428692951 22-23 2.5636843611347404E-4 24-25 0.0013062582221019868 26-27 0.003332789669475163 28-29 0.002795636755713598 30-31 0.00997395296689087 32-33 4.150727060884818E-4 34-35 0.0 36-37 2.4416041534616575E-5 38-39 1.953283322769326E-4 40-41 0.0 42-43 2.8078447764809064E-4 44-45 8.057293706423469E-4 46-47 0.0027101806103424398 48-49 0.0029055089426193725 50-51 0.003039797171059764 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0010010577029192797 64-65 0.0024416041534616574 66-67 0.0029055089426193725 68-69 0.007275980377315739 70-71 6.104010383654144E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.014918201377650729 82-83 0.011292419209760167 84-85 0.016834860638118128 86-87 0.014637416900002639 88-89 0.014112472007008381 90-91 0.013086998262554486 92-93 0.00926588776238699 94-95 0.0260763323589705 96-97 0.010584354005256286 98-99 0.013587527114014125 100-101 0.014051431903171839 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4095668.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.76007551628728 #Duplication Level Percentage of deduplicated Percentage of total 1 60.687868063673925 24.12954217134154 2 16.71249403336824 13.289800496644439 3 7.73701211500801 9.22872557889444 4 4.293403604913376 6.8282420621302355 5 2.6600661339039844 5.288221518117038 6 1.7379990033785935 4.146178297293894 7 1.2176676566098341 3.3890190586383295 8 0.8738135627747794 2.779431459446501 9 0.6753734564960504 2.416760966878104 >10 3.2347239584165104 21.587049540879896 >50 0.12174651747973264 3.2515941013073335 >100 0.04610425305995856 3.1274850558314027 >500 0.001542536545568776 0.43385810020402404 >1k 1.8510437126357398E-4 0.10409159239279363 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 2.4416041534616575E-5 0.0 6 0.0 0.0 0.0 2.4416041534616575E-5 0.0 7 0.0 0.0 0.0 2.4416041534616575E-5 0.0 8 0.0 0.0 0.0 2.4416041534616575E-5 0.0 9 0.0 0.0 0.0 2.4416041534616575E-5 0.0 10-11 0.0 0.0 0.0 2.4416041534616575E-5 0.0 12-13 0.0 0.0 0.0 7.324812460384973E-5 0.0 14-15 0.0 0.0 0.0 9.76641661384663E-5 0.0 16-17 0.0 0.0 0.0 1.953283322769326E-4 0.0 18-19 0.0 0.0 0.0 2.929924984153989E-4 0.0 20-21 0.0 0.0 0.0 3.540326022519404E-4 0.0 22-23 0.0 0.0 0.0 5.005288514596399E-4 0.0 24-25 0.0 0.0 0.0 7.446892668058055E-4 0.0 26-27 0.0 2.4416041534616575E-5 0.0 0.0010132657236865878 0.0 28-29 0.0 2.4416041534616575E-5 0.0 0.001562626658215461 0.0 30-31 0.0 2.4416041534616575E-5 0.0 0.003650198209425178 0.0 32-33 0.0 2.4416041534616575E-5 0.0 0.010108241195331262 0.0 34-35 0.0 2.4416041534616575E-5 0.0 0.018556191566308597 0.0 36-37 0.0 2.4416041534616575E-5 0.0 0.033144776383242 0.0 38-39 0.0 2.4416041534616575E-5 0.0 0.06543499131277242 0.0 40-41 0.0 2.4416041534616575E-5 0.0 0.10789448754147064 0.0 42-43 0.0 2.4416041534616575E-5 0.0 0.15104984095390545 0.0 44-45 0.0 2.4416041534616575E-5 0.0 0.19772110434732504 0.0 46-47 0.0 2.4416041534616575E-5 0.0 0.2530356464439989 0.0 48-49 0.0 2.4416041534616575E-5 0.0 0.31627319401865583 0.0 50-51 0.0 2.4416041534616575E-5 0.0 0.3892527421656248 0.0 52-53 0.0 2.4416041534616575E-5 0.0 0.4664684735188497 0.0 54-55 0.0 2.4416041534616575E-5 0.0 0.543464460498263 0.0 56-57 0.0 2.4416041534616575E-5 0.0 0.6324853479334751 0.0 58-59 0.0 2.4416041534616575E-5 0.0 0.7347031058181474 0.0 60-61 0.0 2.4416041534616575E-5 0.0 0.8535359799671263 0.0 62-63 0.0 2.4416041534616575E-5 0.0 0.9814272055254478 0.0 64-65 0.0 2.4416041534616575E-5 0.0 1.110099744412877 0.0 66-67 0.0 2.4416041534616575E-5 0.0 1.2529335873903842 0.0 68-69 0.0 2.4416041534616575E-5 0.0 1.4165088576515479 0.0 70-71 0.0 2.4416041534616575E-5 0.0 1.6017289487331494 0.0 72-73 0.0 2.4416041534616575E-5 0.0 1.8068114896031613 0.0 74-75 0.0 2.4416041534616575E-5 0.0 2.0151169479557423 0.0 76-77 0.0 2.4416041534616575E-5 0.0 2.2282567825321777 0.0 78-79 0.0 2.4416041534616575E-5 0.0 2.4747123057825977 0.0 80-81 0.0 2.4416041534616575E-5 0.0 2.7560583523859847 0.0 82-83 0.0 2.4416041534616575E-5 0.0 3.064518413113563 0.0 84-85 0.0 2.4416041534616575E-5 0.0 3.3834041235764225 0.0 86-87 0.0 2.4416041534616575E-5 0.0 3.7085037166098425 0.0 88-89 0.0 2.4416041534616575E-5 0.0 4.070764524858949 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4275 0.0 32.445736 1 TCAACGC 3965 0.0 32.34444 4 CAACGCA 4270 0.0 30.145363 5 ATCAACG 4280 0.0 29.963953 3 GGTATCA 2420 0.0 29.050743 1 AACGCAG 4665 0.0 27.695019 6 GTACATG 9370 0.0 24.790207 1 ACATGGG 8670 0.0 24.105309 3 CATGGGG 4945 0.0 24.013353 4 TATCAAC 5495 0.0 23.686113 2 TACATGG 9510 0.0 23.576124 2 CGCAGAG 5470 0.0 23.185066 8 ACGCAGA 5680 0.0 22.495123 7 AGAGTAC 6005 0.0 17.717974 10-11 GACCCGT 300 7.695962E-8 17.416298 6 GTATTAT 2375 0.0 17.200686 1 GAGTACA 6415 0.0 16.808931 1 GTATTAC 1345 0.0 16.599174 1 TATAAGA 2975 0.0 16.126692 2 GCAGAGT 7885 0.0 16.023758 9 >>END_MODULE