FastQCFastQC Report
Fri 10 Feb 2017
SRR2568632_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568632_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4095668
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC38750.032.246994
GTATCAA43550.031.6755681
GGTATCA24400.030.9971561
CAACGCA40550.030.8155575
ATCAACG42250.029.6880993
AACGCAG43700.028.7030226
GTACATG80900.024.9893511
TATCAAC53900.024.0905112
ACATGGG75500.023.9134253
CGCAGAG51850.023.5427368
ACGCAGA53400.023.0434637
TACATGG82550.022.9317972
AGTCGCG600.004767282419.79070718-19
CATGGGG40650.018.5841064
CCTATAC18600.018.1363773
AGAGTAC59100.017.27919810-11
GTATTAA32000.016.3514881
GTATTAT25750.015.8867761
GTATAGA23550.015.5530081
GTATATA48400.015.2335421
Produced by FastQC (version 0.11.5)