FastQCFastQC Report
Fri 10 Feb 2017
SRR2568629_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568629_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3932763
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40700.034.430881
GGTATCA24700.034.232891
TCAACGC40650.032.7203564
CAACGCA42900.031.0042535
GTACATG94600.030.9321481
ATCAACG44550.029.8551863
AACGCAG45000.029.6633266
TACATGG94050.029.6479242
ACATGGG94950.028.415993
TATCAAC53950.025.1819632
ACGCAGA54150.024.5632387
CGCAGAG56650.023.4792488
CATGGGG62350.021.8658314
GTACCGT4000.020.1890076
GTATATA32000.018.7042851
GTATAAG16650.018.5446931
ACCGTAT3357.3305273E-1018.4342148
AGAGTAC64700.018.2813110-11
GTATTAA31050.018.0526491
TATACTG24700.017.5010835
Produced by FastQC (version 0.11.5)