##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568629_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3932763 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.71926556469332 33.0 31.0 34.0 30.0 34.0 2 31.84704977136939 34.0 31.0 34.0 30.0 34.0 3 31.879711795498483 34.0 31.0 34.0 30.0 34.0 4 35.25593049975297 37.0 35.0 37.0 32.0 37.0 5 35.29930967108875 37.0 35.0 37.0 32.0 37.0 6 35.40703139243326 37.0 35.0 37.0 33.0 37.0 7 35.37226677529259 37.0 35.0 37.0 33.0 37.0 8 35.38664928448523 37.0 35.0 37.0 33.0 37.0 9 37.14766056332405 39.0 37.0 39.0 33.0 39.0 10-11 37.100296915934166 39.0 37.0 39.0 33.0 39.0 12-13 37.06607708117677 39.0 37.0 39.0 33.0 39.0 14-15 38.42066048729608 40.5 38.0 41.0 33.0 41.0 16-17 38.37540184852227 40.5 38.0 41.0 33.0 41.0 18-19 38.34707519878518 40.0 38.0 41.0 33.0 41.0 20-21 38.341212145252584 40.0 38.0 41.0 33.0 41.0 22-23 38.27356326836883 40.0 38.0 41.0 33.0 41.0 24-25 38.213619788428645 40.0 38.0 41.0 33.0 41.0 26-27 38.110532467885804 40.0 38.0 41.0 32.5 41.0 28-29 37.98153461065414 40.0 38.0 41.0 32.0 41.0 30-31 37.90381787562586 40.0 38.0 41.0 32.0 41.0 32-33 37.79316755166787 40.0 38.0 41.0 32.0 41.0 34-35 37.642378144831 40.0 38.0 41.0 31.0 41.0 36-37 37.5284558210093 40.0 38.0 41.0 31.0 41.0 38-39 37.42804003190632 40.0 37.0 41.0 31.0 41.0 40-41 37.30371395377753 40.0 37.0 41.0 30.0 41.0 42-43 37.154220200912185 40.0 37.0 41.0 30.0 41.0 44-45 36.97385171697354 40.0 37.0 41.0 30.0 41.0 46-47 37.05089322697553 40.0 36.5 41.0 30.0 41.0 48-49 37.25317645634888 40.0 37.0 41.0 30.0 41.0 50-51 36.18387581453548 39.0 35.5 40.0 29.0 40.5 52-53 36.34302778987699 39.0 35.5 40.0 29.5 40.5 54-55 36.95913471012619 40.0 36.0 41.0 30.0 41.0 56-57 36.9498920987611 40.0 36.0 41.0 30.0 41.0 58-59 36.80297287174437 40.0 36.0 41.0 29.0 41.0 60-61 36.5941842923156 39.0 35.5 41.0 29.0 41.0 62-63 36.333459580452725 39.0 35.0 41.0 28.5 41.0 64-65 36.1647312589139 39.0 35.0 41.0 28.5 41.0 66-67 36.03999554511675 38.5 35.0 41.0 29.0 41.0 68-69 35.746012536224534 37.5 35.0 40.0 28.0 41.0 70-71 35.383121993367006 37.0 35.0 40.0 28.0 41.0 72-73 35.01606631266618 36.5 34.0 39.0 28.0 41.0 74-75 34.64612525595872 36.0 34.0 39.0 27.5 41.0 76-77 34.27051146992585 35.5 34.0 38.5 27.0 40.0 78-79 33.90252756141166 35.0 34.0 37.0 26.0 39.0 80-81 33.531140320431206 35.0 34.0 37.0 26.0 39.0 82-83 33.21169226317477 35.0 33.0 36.0 26.0 38.5 84-85 32.9160677874563 35.0 33.0 36.0 26.0 37.0 86-87 32.67421759205932 35.0 33.0 36.0 26.0 37.0 88-89 32.45914208407677 35.0 33.0 35.0 25.0 36.5 90-91 32.28248155304553 35.0 33.0 35.0 25.0 36.0 92-93 32.133335520091094 35.0 33.0 35.0 25.0 36.0 94-95 31.993989848867066 35.0 32.0 35.0 25.0 36.0 96-97 31.89563075629017 35.0 32.0 35.0 24.0 35.5 98-99 31.759736729622404 35.0 32.0 35.0 24.0 35.0 100-101 30.450305675678905 34.0 29.5 35.0 20.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4206154491517644 1101 2 0.38191663918407315 1101 3 0.41162191067853016 1101 4 0.31012667683584993 1101 5 0.3486174003264466 1101 6 0.27614205615700627 1101 7 0.3324402256214398 1101 8 0.34787294470356045 1101 9 0.4515077823095055 1101 10-11 0.3998647516769296 1101 12-13 0.4025437456220473 1101 14-15 0.5403557496475955 1101 16-17 0.5097511500763687 1101 18-19 0.5442376575353194 1101 20-21 0.35367445689860944 1101 22-23 0.38613936233175394 1101 24-25 0.38381133612188734 1101 26-27 0.501111604594513 1101 28-29 0.5726013730389354 1101 30-31 0.5271180901911308 1101 32-33 0.5310906524525194 1101 34-35 0.28506703459720484 1101 36-37 0.4057750775021063 1101 38-39 0.4465982094021399 1101 40-41 0.46353563577483214 1101 42-43 0.4102496546164076 1101 44-45 0.5933382190615006 1101 46-47 0.4907737624339319 1101 48-49 0.5194124229333923 1101 50-51 0.38737273990117416 1101 52-53 0.3829522731860209 1101 54-55 0.4981802899993042 1101 56-57 0.4572907022608632 1101 58-59 0.3767224892637415 1101 60-61 0.46525729620427825 1101 62-63 0.4795144265284321 1101 64-65 0.5391569905609117 1101 66-67 0.3895630071064389 1101 68-69 0.357011453795387 1101 70-71 0.49870788205027594 1101 72-73 0.3861415535100434 1101 74-75 0.34945896931644427 1101 76-77 0.23575573935959682 1101 78-79 0.24737506975629486 1101 80-81 0.15546718127713177 1101 82-83 0.19214062159314693 1101 84-85 0.11750724695929193 1101 86-87 0.18676537882733157 1101 88-89 0.2684836341639283 1101 90-91 0.3485748716982897 1101 92-93 0.26699545933820445 1101 94-95 0.21896382493487465 1101 96-97 0.26797950145117255 1101 98-99 0.30564920942662255 1101 100-101 0.051885010056242464 1102 1 0.29102476126311316 1102 2 0.21168001105342782 1102 3 0.38186109266288426 1102 4 0.2750967695787736 1102 5 0.35123863230547414 1102 6 0.3128311532664796 1102 7 0.32518266241084604 1102 8 0.33495859552328966 1102 9 0.38819639038315756 1102 10-11 0.4330480053517647 1102 12-13 0.38965126721026166 1102 14-15 0.44338293569202847 1102 16-17 0.4102443155291411 1102 18-19 0.48145619025398645 1102 20-21 0.42766408173206827 1102 22-23 0.3428956909985956 1102 24-25 0.31153265743145653 1102 26-27 0.413254010737667 1102 28-29 0.6183775706876418 1102 30-31 0.551863981746898 1102 32-33 0.5127617258752721 1102 34-35 0.438080228783555 1102 36-37 0.3358110325021215 1102 38-39 0.3060153547805342 1102 40-41 0.32694779624812753 1102 42-43 0.14881056348404798 1102 44-45 0.30384966358636234 1102 46-47 0.3339672804271956 1102 48-49 0.3831860623814052 1102 50-51 0.41925352818343953 1102 52-53 0.4332164612018232 1102 54-55 0.42422829375509963 1102 56-57 0.4957881451469106 1102 58-59 0.461538416182222 1102 60-61 0.45897196336650126 1102 62-63 0.5013832932042348 1102 64-65 0.47187028081562943 1102 66-67 0.4435536480476472 1102 68-69 0.4420356284659377 1102 70-71 0.46644129884571583 1102 72-73 0.45537041466516825 1102 74-75 0.4296612508315363 1102 76-77 0.4896062172019313 1102 78-79 0.46243631334177593 1102 80-81 0.4394713359080882 1102 82-83 0.4518284211275798 1102 84-85 0.45748820716481475 1102 86-87 0.3713568383058288 1102 88-89 0.3769612874005901 1102 90-91 0.28386660329804414 1102 92-93 0.40720759764187875 1102 94-95 0.4132242017315306 1102 96-97 0.35868260454644485 1102 98-99 0.3823733595367038 1102 100-101 0.19962626229222025 1103 1 0.2741595903530403 1103 2 0.25283975934670266 1103 3 0.3601976341183537 1103 4 0.2605004884102087 1103 5 0.2955091798015985 1103 6 0.3112276318330416 1103 7 0.31599796253620127 1103 8 0.3835513928169192 1103 9 0.40129775139322277 1103 10-11 0.3885884646555624 1103 12-13 0.4136894511487199 1103 14-15 0.4220706437545374 1103 16-17 0.4651353122271189 1103 18-19 0.4265517027143986 1103 20-21 0.2983275856058256 1103 22-23 0.4011222308297704 1103 24-25 0.4304504464012382 1103 26-27 0.47315510128334637 1103 28-29 0.2587775687449607 1103 30-31 0.34517531691390246 1103 32-33 0.3133598018654027 1103 34-35 0.3528724104630925 1103 36-37 0.3564164717936009 1103 38-39 0.24880710367388303 1103 40-41 0.2934702053454856 1103 42-43 0.3225431933509384 1103 44-45 0.28380632021044505 1103 46-47 0.31042421037572865 1103 48-49 0.37416460169021803 1103 50-51 0.33441252584628955 1103 52-53 0.32057946845071683 1103 54-55 0.3742777107079007 1103 56-57 0.3628342147521053 1103 58-59 0.4318584971920316 1103 60-61 0.34270067279481253 1103 62-63 0.3658240114782174 1103 64-65 0.30284723629962684 1103 66-67 0.261912012288505 1103 68-69 0.2407639412635163 1103 70-71 0.3361942383652021 1103 72-73 0.28067170563078037 1103 74-75 0.2927760891923725 1103 76-77 0.22929982130185778 1103 78-79 0.22656365285607194 1103 80-81 0.3085431902890221 1103 82-83 0.35489652181675524 1103 84-85 0.3372728690492508 1103 86-87 0.2884784161514915 1103 88-89 0.29911434207788545 1103 90-91 0.2970221300371634 1103 92-93 0.1615748528869858 1103 94-95 0.1629806245863179 1103 96-97 0.2108862525779429 1103 98-99 0.2828900048069052 1103 100-101 0.18220675270050535 1104 1 0.3101023207789062 1104 2 0.27890348559853706 1104 3 0.35152690135514675 1104 4 0.328086395473008 1104 5 0.33123591449835743 1104 6 0.21888181410470509 1104 7 0.34193352780783215 1104 8 0.32348804109825124 1104 9 0.3466670414920898 1104 10-11 0.3197726404842527 1104 12-13 0.2930116225502175 1104 14-15 0.3720996891838766 1104 16-17 0.4490568089154223 1104 18-19 0.44667492043664225 1104 20-21 0.3858442053504163 1104 22-23 0.35054447190604066 1104 24-25 0.3925978208538865 1104 26-27 0.43813203296583225 1104 28-29 0.4920018235761745 1104 30-31 0.5087763663447689 1104 32-33 0.508881237827147 1104 34-35 0.3985387045733759 1104 36-37 0.343746819931539 1104 38-39 0.2672065726854811 1104 40-41 0.44732657253453567 1104 42-43 0.37437732523589773 1104 44-45 0.5399205214618021 1104 46-47 0.4358910425117273 1104 48-49 0.5710558116656088 1104 50-51 0.5027748051112368 1104 52-53 0.47311445969294397 1104 54-55 0.5535550883642486 1104 56-57 0.5006448931117902 1104 58-59 0.4587346783980806 1104 60-61 0.4411911670718496 1104 62-63 0.5305577535824852 1104 64-65 0.5377903308476419 1104 66-67 0.3810956617676311 1104 68-69 0.46827873973844447 1104 70-71 0.5516388423135012 1104 72-73 0.4719379993720594 1104 74-75 0.4454002659804601 1104 76-77 0.4465907340755564 1104 78-79 0.4132588496724594 1104 80-81 0.4554720446695484 1104 82-83 0.3657233242114941 1104 84-85 0.45617126974518385 1104 86-87 0.45892161461202363 1104 88-89 0.3137723670703991 1104 90-91 0.3698875776128645 1104 92-93 0.3362353383067429 1104 94-95 0.3661632208391623 1104 96-97 0.3021128197099827 1104 98-99 0.2001424890543042 1104 100-101 0.20123315623455795 1105 1 0.20021895334329187 1105 2 0.2024042578739902 1105 3 0.3044491009659147 1105 4 0.1868492925655687 1105 5 0.2814271556269077 1105 6 0.11398006290419005 1105 7 0.21848883688922172 1105 8 0.2306817244495818 1105 9 0.247540004262774 1105 10-11 0.23495375272748475 1105 12-13 0.35745937388215765 1105 14-15 0.38300316354424524 1105 16-17 0.5049937979018253 1105 18-19 0.4322677494264511 1105 20-21 0.42894911357966237 1105 22-23 0.43172408679554763 1105 24-25 0.38402412893302795 1105 26-27 0.4540439611742926 1105 28-29 0.46887196562117595 1105 30-31 0.44673983572629794 1105 32-33 0.3397917825103889 1105 34-35 0.38227649924342444 1105 36-37 0.4376220139258322 1105 38-39 0.36395015224928784 1105 40-41 0.4376654945694156 1105 42-43 0.436415244006767 1105 44-45 0.4910080463212907 1105 46-47 0.5152942850506363 1105 48-49 0.4814875918414927 1105 50-51 0.4979657824908301 1105 52-53 0.4777680092448975 1105 54-55 0.5352175952008977 1105 56-57 0.4549418263129823 1105 58-59 0.5454002715602257 1105 60-61 0.4485872430426596 1105 62-63 0.5374180027280815 1105 64-65 0.5068650717489902 1105 66-67 0.47228987076600504 1105 68-69 0.3870722872359309 1105 70-71 0.35634430876509526 1105 72-73 0.41486503900750904 1105 74-75 0.40961184631792946 1105 76-77 0.2808610702768064 1105 78-79 0.23832897450437684 1105 80-81 0.2854918582314312 1105 82-83 0.27605423197987733 1105 84-85 0.26403941007443166 1105 86-87 0.3042416438927944 1105 88-89 0.23616122927592187 1105 90-91 0.22101933826207443 1105 92-93 0.25504739450285996 1105 94-95 0.21850359950862952 1105 96-97 0.24026415238255083 1105 98-99 0.26529033191174634 1105 100-101 0.0705640575248907 1106 1 0.31868296620559633 1106 2 0.2031687732487093 1106 3 0.3480074324580009 1106 4 0.34338778918667856 1106 5 0.2529202832222168 1106 6 0.25518658243469616 1106 7 0.2671040817467798 1106 8 0.2817540435373118 1106 9 0.42312071877177004 1106 10-11 0.28638069296626867 1106 12-13 0.35944304713294883 1106 14-15 0.41590721650673146 1106 16-17 0.4209719258028599 1106 18-19 0.4405490088907129 1106 20-21 0.44908376222119983 1106 22-23 0.4103726071883784 1106 24-25 0.42941839276227967 1106 26-27 0.405632231483672 1106 28-29 0.36956053779886133 1106 30-31 0.29170813891026626 1106 32-33 0.2752733577471318 1106 34-35 0.4357240569110701 1106 36-37 0.38120564072433893 1106 38-39 0.4597173016866236 1106 40-41 0.4546401630347958 1106 42-43 0.3655391987406986 1106 44-45 0.48043245316318206 1106 46-47 0.4558919487752817 1106 48-49 0.2663443880452334 1106 50-51 0.32648089573833516 1106 52-53 0.4365330441292201 1106 54-55 0.4796606395214553 1106 56-57 0.5066503227149965 1106 58-59 0.46359827202103077 1106 60-61 0.40743422097309434 1106 62-63 0.4183032067136381 1106 64-65 0.4467844231810574 1106 66-67 0.42432437090052844 1106 68-69 0.4075169161466974 1106 70-71 0.4840192713090019 1106 72-73 0.30784573825197725 1106 74-75 0.2916145400926453 1106 76-77 0.393044644465661 1106 78-79 0.3306116650543629 1106 80-81 0.37658463837963296 1106 82-83 0.2272202933395775 1106 84-85 0.3106569128226937 1106 86-87 0.2523135945410928 1106 88-89 0.2598256578604605 1106 90-91 0.15552149899531287 1106 92-93 0.18554125511075625 1106 94-95 0.23714401366145665 1106 96-97 0.29308458978729846 1106 98-99 0.2589234037671737 1106 100-101 0.21375321214615894 1107 1 0.025438004961781502 1107 2 0.01159097672728393 1107 3 0.10048678078469564 1107 4 0.029333948986817404 1107 5 0.09084863145579902 1107 6 0.08956995516656008 1107 7 0.1812833535040994 1107 8 0.08410468419789652 1107 9 0.07209566539973622 1107 10-11 0.057914804867913006 1107 12-13 0.10275464240522325 1107 14-15 0.18242926864216003 1107 16-17 0.08145517026108706 1107 18-19 0.020586691869702634 1107 20-21 0.02049377441355915 1107 22-23 0.03865952224393965 1107 24-25 0.10940158888156049 1107 26-27 0.1517342567609603 1107 28-29 0.22105426497238057 1107 30-31 0.24546265455930438 1107 32-33 0.05204763814184332 1107 34-35 0.07735041237872053 1107 36-37 0.11492641304882056 1107 38-39 0.15299489915621223 1107 40-41 0.008214379738625155 1107 42-43 0.17588681467897516 1107 44-45 0.25302178717607404 1107 46-47 0.14588017856713265 1107 48-49 0.15689084653610763 1107 50-51 0.02464679217263921 1107 52-53 0.10709686302308086 1107 54-55 0.12384283539897467 1107 56-57 0.1702294898508896 1107 58-59 0.20418409732749865 1107 60-61 0.18635236070941374 1107 62-63 0.1731074327629969 1107 64-65 0.1554392779400473 1107 66-67 0.17202975704909562 1107 68-69 0.19683075510746306 1107 70-71 0.1999004131357296 1107 72-73 0.21019691997587842 1107 74-75 0.27989394923248057 1107 76-77 0.2044757545599154 1107 78-79 0.19746805269923584 1107 80-81 0.15623817829529685 1107 82-83 0.2429355368293642 1107 84-85 0.15634330634077287 1107 86-87 0.16513961343169825 1107 88-89 0.07811474549333752 1107 90-91 0.12832102718810035 1107 92-93 0.11862411973064724 1107 94-95 0.022928900918728345 1107 96-97 0.02470166631636772 1107 98-99 0.0965879390407558 1107 100-101 0.14619108172659168 1108 1 0.23872672574594134 1108 2 0.20476426199391184 1108 3 0.2958869041396319 1108 4 0.2368839597947172 1108 5 0.1722323929287768 1108 6 0.1424880755258897 1108 7 0.23162151382124563 1108 8 0.19025015940270862 1108 9 0.27159060426744475 1108 10-11 0.15725720335901627 1108 12-13 0.19013183059583838 1108 14-15 0.2596439666261503 1108 16-17 0.22617789355771123 1108 18-19 0.23814577803752712 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-0.3609381073267741 2316 82-83 -0.45176841763186815 2316 84-85 -0.5039611463010871 2316 86-87 -0.4670570835603698 2316 88-89 -0.35393638794739246 2316 90-91 -0.36454570324180935 2316 92-93 -0.3458500700537499 2316 94-95 -0.24213160852422533 2316 96-97 -0.3120393383475708 2316 98-99 -0.37603293615679334 2316 100-101 -0.20594264342834379 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 19.0 7 320.0 8 827.0 9 859.0 10 1400.0 11 2951.0 12 5634.0 13 8735.0 14 10672.0 15 12212.0 16 13555.0 17 14893.0 18 16755.0 19 18826.0 20 20661.0 21 22848.0 22 24936.0 23 26323.0 24 27444.0 25 30119.0 26 32979.0 27 36778.0 28 42628.0 29 49508.0 30 58843.0 31 70697.0 32 88344.0 33 114298.0 34 159093.0 35 236512.0 36 410705.0 37 824851.0 38 1191664.0 39 355658.0 40 216.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.47526709241421 18.876454723595522 11.282369078055014 25.36590910593526 2 14.871428037412649 20.362306918278016 41.06826579854326 23.69799924576608 3 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21.74496111849362 22-23 22.44566908871955 27.386534925161822 27.64898473130266 22.518811254815965 24-25 22.293831210110103 27.38045108958222 27.89742416151753 22.428293538790143 26-27 22.427448416104518 27.661837332237198 27.67383957452051 22.236874677137774 28-29 22.415990019905262 27.609234842451407 27.659862565185417 22.31491257245791 30-31 22.619395318953554 27.44847200922512 27.692323928627605 22.239808743193716 32-33 22.36497512235403 27.520225999762253 27.669358381131765 22.445440496751953 34-35 22.65431453662476 27.588110445506125 27.710289178371543 22.047285839497572 36-37 22.629815221512203 27.407397801494778 27.650382186773015 22.312404790220004 38-39 22.563615335139446 27.66156375989016 27.59200698441056 22.18281392055983 40-41 22.688349132658132 27.533746630549565 27.587449332695613 22.190454904096686 42-43 22.515250143029686 27.425694488589407 27.671934397050407 22.387120971330493 44-45 22.662516531166826 27.49563088984401 27.534153816579504 22.307698762409657 46-47 22.541507072978657 27.525661495335324 27.431958609006717 22.500872822679302 48-49 22.439294549687652 27.484560614162966 27.620232937848066 22.455911898301316 50-51 22.591339865811644 27.5493746958919 27.476687535353463 22.382597902942997 52-53 22.673804650826913 27.50158094957667 27.453357855533124 22.371256544063296 54-55 22.553977953921965 27.46519431758283 27.565645323656675 22.415182404838532 56-57 22.563932787203296 27.609266055442443 27.460579241617154 22.366221915737103 58-59 22.62330580307026 27.55733055869372 27.45832891531984 22.36103472291618 60-61 22.694019446379045 27.61310559522656 27.5218211725446 22.171053785849796 62-63 22.589191347799435 27.559643660523705 27.526307873411987 22.32485711826487 64-65 22.519688763505094 27.448392446136328 27.61031943837169 22.42159935198689 66-67 22.550315330211497 27.630397367050264 27.383056120170163 22.436231182568076 68-69 22.544520533140204 27.58353107098237 27.421149278086986 22.45079911779044 70-71 22.486092702602196 27.340065285313347 27.58896315861407 22.58487885347039 72-73 22.50940369404411 27.502000501937186 27.442373211912336 22.546222592106364 74-75 22.463214793263667 27.521299910520924 27.473254808387892 22.542230487827513 76-77 22.46538883731361 27.48420131088499 27.480705041214026 22.569704810587364 78-79 22.425467794525122 27.380991430198055 27.487532302353333 22.70600847292349 80-81 22.453326239742353 27.477742671510114 27.363633173185004 22.705297915562532 82-83 22.547545259689706 27.460789600535772 27.520678527556345 22.47098661221818 84-85 22.572544302252243 27.41292140546479 27.474351996907487 22.540182295375473 86-87 22.379071430533276 27.4467365994635 27.545766241217283 22.62842572878594 88-89 22.46752549869512 27.3800138448009 27.60050323460732 22.551957421896656 90-91 22.47238915959566 27.396074941829585 27.63377923763474 22.49775666094001 92-93 22.508222113664917 27.369612776781914 27.571500537968962 22.550664571584203 94-95 22.322120954317533 27.432648789426768 27.665785394277197 22.5794448619785 96-97 22.374886152638812 27.320365933995934 27.672992360671998 22.631755552693257 98-99 22.3214575221051 27.308067368728366 27.63541755013535 22.73505755903118 100-101 22.50957775237754 27.17949259179339 27.656495114198698 22.65443454163037 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 18.0 1 20.0 2 30.5 3 43.0 4 42.0 5 49.5 6 74.5 7 119.5 8 174.5 9 195.0 10 215.0 11 264.0 12 306.0 13 356.5 14 434.0 15 503.0 16 588.5 17 692.5 18 788.5 19 907.5 20 1084.0 21 1347.5 22 1665.0 23 2244.5 24 3326.5 25 5179.0 26 8688.0 27 13886.0 28 20802.0 29 29981.5 30 40663.0 31 51804.5 32 63995.0 33 76941.0 34 89868.0 35 100714.0 36 111838.0 37 122667.0 38 130826.5 39 137451.0 40 144195.5 41 151119.0 42 155405.5 43 159885.0 44 165241.5 45 169651.0 46 170691.5 47 168079.5 48 165873.0 49 162184.5 50 155543.5 51 147072.5 52 138976.5 53 129992.5 54 119193.5 55 108097.5 56 95374.0 57 82906.0 58 72266.0 59 61159.0 60 48930.0 61 37964.5 62 28846.0 63 21080.5 64 14751.5 65 10733.5 66 7778.0 67 5460.0 68 3849.5 69 2718.5 70 1942.5 71 1429.5 72 1045.0 73 713.5 74 519.0 75 385.5 76 260.5 77 172.5 78 117.0 79 75.5 80 54.5 81 44.0 82 35.5 83 27.0 84 16.0 85 16.0 86 18.0 87 18.0 88 17.0 89 14.5 90 10.0 91 6.0 92 5.5 93 4.0 94 2.0 95 2.5 96 3.5 97 2.5 98 1.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005822878215646354 2 0.007526515073499216 3 0.0010679514631316456 4 5.594031473546715E-4 5 0.003432701131494575 6 0.005619458889335564 7 0.005568604057757867 8 0.004882063831458952 9 0.00902673260504129 10-11 0.00785707147875425 12-13 0.00411924135779349 14-15 0.0 16-17 0.0 18-19 1.3985078683866788E-4 20-21 0.0011442337104981917 22-23 2.7970157367733576E-4 24-25 0.0016019271946974684 26-27 0.004182809897265612 28-29 0.003432701131494575 30-31 0.011658470139187131 32-33 3.9412494472715494E-4 34-35 0.0 36-37 0.0 38-39 1.1442337104981918E-4 40-41 0.0 42-43 3.3055640525503317E-4 44-45 0.0010170966315539481 46-47 0.0030512898946618446 48-49 0.0032038543893949368 50-51 0.003979390570954823 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0013349393289145569 64-65 0.0032165680972893614 66-67 0.0035471245025443946 68-69 0.009255579347140929 70-71 6.483991026156419E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.014620764078588006 82-83 0.012294155533908347 84-85 0.017366924983783668 86-87 0.015434441383831165 88-89 0.015281876889098071 90-91 0.014404631044382792 92-93 0.009662417999762508 94-95 0.024105190167828573 96-97 0.01053966384447779 98-99 0.014811469697004372 100-101 0.015345445428570194 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3932763.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.619707413773256 #Duplication Level Percentage of deduplicated Percentage of total 1 63.05106017239473 28.763709171928593 2 16.80145090563246 15.32954548883657 3 7.35467424699171 10.06554261814125 4 4.076288610872345 7.4383637504837035 5 2.390585106633196 5.452889655616517 6 1.5696761034047355 4.296489874302944 7 1.0648809415738663 3.4005689889572213 8 0.771383565846614 2.815223406217251 9 0.553513251053499 2.2726001306436343 >10 2.287017035159758 16.598663451735774 >50 0.05764475049201108 1.775072089080227 >100 0.02132109177670085 1.6115301832078024 >500 3.9216969811133626E-4 0.11953520949275298 >1k 1.1204847039533006E-4 0.060265981355673466 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 2.5427415788848707E-5 0.0 7 0.0 0.0 0.0 2.5427415788848707E-5 0.0 8 0.0 0.0 0.0 2.5427415788848707E-5 0.0 9 0.0 0.0 0.0 2.5427415788848707E-5 0.0 10-11 0.0 0.0 0.0 3.814112368327306E-5 0.0 12-13 0.0 0.0 0.0 8.899595526097047E-5 0.0 14-15 0.0 0.0 0.0 1.0170966315539483E-4 0.0 16-17 0.0 0.0 0.0 1.7799191052194092E-4 0.0 18-19 0.0 0.0 0.0 1.907056184163653E-4 0.0 20-21 0.0 0.0 0.0 2.0341932631078965E-4 2.5427415788848707E-5 22-23 0.0 0.0 0.0 2.669878657829114E-4 2.5427415788848707E-5 24-25 0.0 0.0 0.0 3.686975289383062E-4 2.5427415788848707E-5 26-27 0.0 0.0 0.0 4.1955236051600367E-4 2.5427415788848707E-5 28-29 0.0 0.0 0.0 5.212620236713985E-4 2.5427415788848707E-5 30-31 0.0 0.0 0.0 7.755361815598855E-4 2.5427415788848707E-5 32-33 0.0 0.0 0.0 0.0012713707894424352 2.5427415788848707E-5 34-35 0.0 0.0 0.0 0.002021479555213472 2.5427415788848707E-5 36-37 0.0 0.0 0.0 0.0036234067499109407 2.5427415788848707E-5 38-39 0.0 0.0 0.0 0.007246813499821881 2.5427415788848707E-5 40-41 0.0 0.0 0.0 0.013463816660195388 2.5427415788848707E-5 42-43 0.0 0.0 0.0 0.019642678696885622 2.5427415788848707E-5 44-45 0.0 0.0 0.0 0.02582154073357586 2.5427415788848707E-5 46-47 0.0 0.0 0.0 0.0339837412017963 2.5427415788848707E-5 48-49 0.0 0.0 0.0 0.04504466706994548 2.5427415788848707E-5 50-51 0.0 0.0 0.0 0.057821943503841955 2.5427415788848707E-5 52-53 0.0 0.0 0.0 0.07118405050088195 2.5427415788848707E-5 54-55 0.0 0.0 0.0 0.08553782671368704 2.5427415788848707E-5 56-57 0.0 0.0 0.0 0.10237077596590488 2.5427415788848707E-5 58-59 0.0 0.0 0.0 0.12458162365746422 2.5427415788848707E-5 60-61 0.0 0.0 0.0 0.15236107540678145 2.5427415788848707E-5 62-63 0.0 0.0 0.0 0.18457761121125277 2.5427415788848707E-5 64-65 0.0 0.0 0.0 0.2197437272472305 2.5427415788848707E-5 66-67 0.0 0.0 0.0 0.261673535883042 2.5427415788848707E-5 68-69 0.0 0.0 0.0 0.31160026678444647 2.5427415788848707E-5 70-71 0.0 0.0 0.0 0.3740881410855421 2.5427415788848707E-5 72-73 0.0 0.0 0.0 0.4427040225917504 3.814112368327306E-5 74-75 0.0 0.0 0.0 0.5124132829768792 5.085483157769741E-5 76-77 1.2713707894424353E-5 0.0 0.0 0.5886446755118475 5.085483157769741E-5 78-79 2.5427415788848707E-5 0.0 0.0 0.6813021786464122 5.085483157769741E-5 80-81 2.5427415788848707E-5 0.0 0.0 0.7924326993515755 5.085483157769741E-5 82-83 2.5427415788848707E-5 0.0 0.0 0.9180314196405936 5.085483157769741E-5 84-85 2.5427415788848707E-5 0.0 0.0 1.0486520545479094 5.085483157769741E-5 86-87 2.5427415788848707E-5 0.0 0.0 1.1879942930708003 5.085483157769741E-5 88-89 2.5427415788848707E-5 0.0 0.0 1.341944581964385 5.085483157769741E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4070 0.0 34.43088 1 GGTATCA 2470 0.0 34.23289 1 TCAACGC 4065 0.0 32.720356 4 CAACGCA 4290 0.0 31.004253 5 GTACATG 9460 0.0 30.932148 1 ATCAACG 4455 0.0 29.855186 3 AACGCAG 4500 0.0 29.663326 6 TACATGG 9405 0.0 29.647924 2 ACATGGG 9495 0.0 28.41599 3 TATCAAC 5395 0.0 25.181963 2 ACGCAGA 5415 0.0 24.563238 7 CGCAGAG 5665 0.0 23.479248 8 CATGGGG 6235 0.0 21.865831 4 GTACCGT 400 0.0 20.189007 6 GTATATA 3200 0.0 18.704285 1 GTATAAG 1665 0.0 18.544693 1 ACCGTAT 335 7.3305273E-10 18.434214 8 AGAGTAC 6470 0.0 18.28131 10-11 GTATTAA 3105 0.0 18.052649 1 TATACTG 2470 0.0 17.501083 5 >>END_MODULE