FastQCFastQC Report
Fri 10 Feb 2017
SRR2568629_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568629_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3932763
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39850.033.4306531
GGTATCA26800.032.6661641
GTACATG90450.032.0875361
TACATGG90300.030.4485592
TCAACGC42450.029.6610414
CAACGCA42900.029.3499095
ATCAACG43400.029.2307363
ACATGGG91300.029.0393
AACGCAG46000.027.2686886
TATCAAC54600.023.9588892
CATGGGG56350.023.6092554
ACGCAGA54500.023.0143137
CGCAGAG56850.022.05688
TATACTG22700.020.7218025
GTATAGA19200.020.0723821
GTATATA30300.019.4712661
ATAATAC22100.019.3494423
GTATAAT24400.017.744621
AGAGTAC64950.017.47784210-11
GTATTAT21500.017.2612111
Produced by FastQC (version 0.11.5)