FastQCFastQC Report
Fri 10 Feb 2017
SRR2568628_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568628_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860262
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT39510.4592786848657734No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA27960.3250172621829164No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA24860.2889817288221495No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC23670.2751487337578552No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA23220.26991776923774385No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG22450.26096700772555337No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC21810.25352741374139504No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA21470.24957512943731097No Hit
TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA15460.17971269217982427No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG15250.17727157540377234No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT15220.17692284443576492No Hit
GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT15120.17576040787574018No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT14580.16948325045160662No Hit
GTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATT14060.16343858033947797No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG13780.1601837579714087No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT13290.1544878188272875No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT12240.14228223494702777No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG11830.13751624505092636No Hit
CCATGTAATTATTGGATCAACATTCCTTATTGTTTGCCTACTACGACAAC11470.13333147343483728No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA10970.12751929063471362No Hit
TTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACATCATTCTCCA10790.1254269048266691No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA10750.1249619302026592No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10280.11949847837054292No Hit
CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG10170.1182197981545157No Hit
CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC10060.11694111793848852No Hit
TGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAA9990.11612741234647118No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC9910.1151974630984514No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT9850.11450000116243655No Hit
GTGATGTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAA9480.11019898589034503No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT9450.1098502549223376No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT8910.10357309749820404No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC8720.10136446803415704No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCGC502.826806E-547.4988378
GTTCGCA554.9629827E-543.180769
CTATAGT3500.042.0728452
GTATCAA37950.040.805061
CTATTAA4700.039.416221
GGTCGAT250.00161692637.99575440-41
TATAGTC3950.037.2797363
GGTATCA20000.036.8137361
AACCCTT5000.036.0991176
ACCCTTG5200.034.710697
AACGCAG46200.033.722126
CTGATTA4400.033.4650889
TCAACGC45500.033.3013844
CAACGCA46000.033.2491875
ATCAACG45500.033.094523
CCTTGGC5250.031.6658949
CTTCGGC902.4126653E-531.6658929
ACCGTTT600.00395087831.665898
TATCAAC48850.030.824992
ATTAACC6800.030.7363263
Produced by FastQC (version 0.11.5)