FastQCFastQC Report
Fri 10 Feb 2017
SRR2568628_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568628_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860262
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT194032.2554756574159964No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT99071.1516259000165068RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT45000.5230964520111314RNA PCR Primer, Index 33 (95% over 24bp)
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT39170.4553264005616894No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA29270.3402451811192404No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA27990.3253659931509238No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC25160.2924690385022237No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC23770.2763111703178799No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG23160.26922030730172897No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA20670.24027563695711304No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA19440.22597766726880883No Hit
GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT17670.20540254015637094No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG16690.19401066186812854RNA PCR Primer, Index 33 (96% over 29bp)
TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA15100.17552792056373523No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT15060.17506294593972535No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT14840.17250558550767092No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG14560.16925076313960166No Hit
GTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATT14430.1677395956115695No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT14180.16483350421150766No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG13650.15867259044337656No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT13200.15344162592326524RNA PCR Primer, Index 33 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC12970.15076802183520832No Hit
CCATGTAATTATTGGATCAACATTCCTTATTGTTTGCCTACTACGACAAC12630.14681573753112426No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT11310.13147157493879771No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT11040.12833299622673092RNA PCR Primer, Index 33 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10970.12751929063471362No Hit
TTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACATCATTCTCCA10840.12600812310668144No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG10630.1235670063306295No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA10360.12042842761856272No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT10000.11624365600247366No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA9890.11496497578644645No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA9700.11275634632239946No Hit
CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC9620.11182639707437968No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT9500.11043147320234999No Hit
CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG9330.10845533105030793No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC9160.10647918889826588No Hit
GTGATGTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAA8930.10380558481020899No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC8840.10275939190618673No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT8770.10194568631416939No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC8680.10089949341014713No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC8670.10078324975414465No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC8670.10078324975414465No Hit
TGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAA8650.10055076244213973No Hit
CTTTGAAACATCATTCTCCATTTCAGATGGTATCTATGGTTCTACATTCT8650.10055076244213973No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG8610.10008578781812982No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATCG200.00207423671.247089
CCGTTTA250.004998595557.0642
GACATAT1151.8189894E-1245.493741
GGTATCA18250.043.2615431
GTATTGG3200.043.102731
GTATCAA34500.041.2200851
CATATAG1051.024091E-940.7244533
GTCATAA500.001605329838.049311
GTTCGCA500.001615915337.9984449
CGCAGTG500.001615915337.9984448
ATGGGCG801.07545075E-535.6338965
ATTAGCC1750.035.2945253
GTCCACG550.002576110434.554086
TGTTCGC550.002579785234.5440378
TTAGACG352.05425E-433.9271890-91
ATTATGC851.6289414E-533.5377853
TCAACGC43200.032.0045174
GGGATCG600.00394670231.6727317
CATCGTA300.00395228631.66536738-39
AACGCAG43700.031.6383326
Produced by FastQC (version 0.11.5)