##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568628_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 860262 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.65767986962111 34.0 31.0 34.0 31.0 34.0 2 32.769438845375014 34.0 31.0 34.0 31.0 34.0 3 32.84591322178592 34.0 31.0 34.0 31.0 34.0 4 36.2415147943301 37.0 37.0 37.0 35.0 37.0 5 36.308442079273526 37.0 37.0 37.0 35.0 37.0 6 36.31997461238553 37.0 37.0 37.0 35.0 37.0 7 36.26628283011455 37.0 37.0 37.0 35.0 37.0 8 36.25454919547766 37.0 37.0 37.0 35.0 37.0 9 38.08744661510098 39.0 39.0 39.0 37.0 39.0 10-11 38.00118161676326 39.0 39.0 39.0 35.0 39.0 12-13 37.99616628422504 39.0 39.0 39.0 35.0 39.0 14-15 39.39176727555093 41.0 39.0 41.0 36.0 41.0 16-17 39.36407338694491 41.0 39.0 41.0 36.0 41.0 18-19 39.48270468764167 41.0 39.5 41.0 36.5 41.0 20-21 39.48332019780021 41.0 39.0 41.0 37.0 41.0 22-23 39.43589046127808 41.0 39.0 41.0 36.0 41.0 24-25 39.38608296077241 41.0 39.0 41.0 36.0 41.0 26-27 39.28526541913975 41.0 39.0 41.0 36.0 41.0 28-29 39.18385154755179 41.0 39.0 41.0 36.0 41.0 30-31 39.15250528327417 41.0 39.0 41.0 36.0 41.0 32-33 38.99278766236333 40.5 39.0 41.0 35.0 41.0 34-35 38.881515166309796 40.0 38.0 41.0 35.0 41.0 36-37 38.80393938125827 40.0 38.0 41.0 35.0 41.0 38-39 38.57403093476174 40.0 38.0 41.0 34.5 41.0 40-41 38.557503411751306 40.0 38.0 41.0 34.0 41.0 42-43 38.47695876372547 40.0 38.0 41.0 34.0 41.0 44-45 38.42032311086622 40.0 38.0 41.0 34.0 41.0 46-47 38.25191860154232 40.0 38.0 41.0 33.5 41.0 48-49 38.153556125924425 40.0 38.0 41.0 33.0 41.0 50-51 38.08544954909085 40.0 38.0 41.0 33.5 41.0 52-53 38.28791461206005 40.0 38.0 41.0 34.0 41.0 54-55 38.211386763567376 40.0 38.0 41.0 34.0 41.0 56-57 38.0445776984221 40.0 37.5 41.0 33.5 41.0 58-59 37.83371403130674 40.0 37.0 41.0 33.0 41.0 60-61 37.699977448730735 40.0 37.0 41.0 33.0 41.0 62-63 37.410388928024254 40.0 36.5 41.0 32.0 41.0 64-65 37.019900332689346 39.0 36.0 41.0 31.0 41.0 66-67 36.5347609216727 39.0 35.5 41.0 30.5 41.0 68-69 35.920347522033985 38.5 35.0 41.0 28.5 41.0 70-71 35.38852872729471 37.0 35.0 40.0 27.5 41.0 72-73 34.95086555026259 37.0 35.0 39.5 26.5 41.0 74-75 34.57311551597071 36.5 35.0 39.0 26.5 41.0 76-77 32.861950196568024 34.5 32.0 36.5 25.0 39.0 78-79 33.74850045683757 35.0 34.0 37.0 26.0 39.0 80-81 33.61460927019908 35.0 34.0 37.0 26.0 39.0 82-83 33.3618665011357 35.0 34.0 37.0 26.0 39.0 84-85 33.02275295200764 35.0 34.0 36.0 26.0 38.0 86-87 32.79765001825025 35.0 34.0 36.0 25.5 37.0 88-89 32.60926671176921 35.0 34.0 35.5 25.0 37.0 90-91 32.468252113890884 35.0 34.0 35.0 25.0 36.5 92-93 32.326683033773435 35.0 34.0 35.0 25.0 36.0 94-95 32.20776519246462 35.0 34.0 35.0 25.0 36.0 96-97 32.103512650797086 35.0 33.5 35.0 25.0 36.0 98-99 32.03306550794991 35.0 33.0 35.0 24.5 35.5 100-101 30.824928335786076 34.5 31.0 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3212831325381913 1101 2 0.20835898374714645 1101 3 0.2535177880362198 1101 4 0.19885014854923355 1101 5 0.13867406242031421 1101 6 0.11864110106439796 1101 7 0.07656926586447099 1101 8 0.20569001797226605 1101 9 0.14800187517939634 1101 10-11 0.14639194387930132 1101 12-13 0.20999693232228367 1101 14-15 0.304025230419704 1101 16-17 0.26439212913977883 1101 18-19 0.40625582092910406 1101 20-21 0.40703904885057085 1101 22-23 0.28682438929428145 1101 24-25 0.35839410401105454 1101 26-27 0.2838129527896385 1101 28-29 0.24649776851369865 1101 30-31 0.2164494983943399 1101 32-33 0.04522764670929291 1101 34-35 0.13698847904255018 1101 36-37 0.1140700411215434 1101 38-39 0.2799127507286485 1101 40-41 0.20932486827274488 1101 42-43 0.146707953101064 1101 44-45 -0.08577485062765078 1101 46-47 -0.014995344901599594 1101 48-49 -0.10911631938517274 1101 50-51 -0.10821475485285958 1101 52-53 -0.14646601439878992 1101 54-55 -0.12271502610843044 1101 56-57 0.025999331291259864 1101 58-59 -0.04147691966358735 1101 60-61 0.057366421858269234 1101 62-63 0.14007212695083382 1101 64-65 -0.05285821197143292 1101 66-67 0.08008704941698852 1101 68-69 0.2841362840943944 1101 70-71 0.42098425948613993 1101 72-73 0.42568879610261234 1101 74-75 0.33901929898791394 1101 76-77 0.2071088927323288 1101 78-79 0.29554542566732067 1101 80-81 0.22647364759598076 1101 82-83 0.12489402757700674 1101 84-85 0.30608474083384607 1101 86-87 0.2947875084820524 1101 88-89 0.23695413038110047 1101 90-91 0.2324134675390468 1101 92-93 0.26032647419922483 1101 94-95 0.35304993892203385 1101 96-97 0.4020187988571564 1101 98-99 0.2867736879515661 1101 100-101 0.08967605817517565 1102 1 0.3140795816969657 1102 2 0.22121380511364208 1102 3 0.21164664347203654 1102 4 0.1909088603182667 1102 5 0.20859135498473336 1102 6 0.1454138941179437 1102 7 0.262747907457495 1102 8 0.23274779999006512 1102 9 0.21191822990778775 1102 10-11 0.2511668228095232 1102 12-13 0.2106780761588425 1102 14-15 0.2742489366506575 1102 16-17 0.35161694298741253 1102 18-19 0.34175938228055713 1102 20-21 0.309073587087596 1102 22-23 0.276491012842051 1102 24-25 0.3774984706079181 1102 26-27 0.305615619154473 1102 28-29 0.3436820371228677 1102 30-31 0.2621619303810121 1102 32-33 0.2847234789983375 1102 34-35 0.2896233131830215 1102 36-37 0.31183823392770194 1102 38-39 0.35790877301033674 1102 40-41 0.16263518689444822 1102 42-43 0.059898495509848715 1102 44-45 0.10782361002770102 1102 46-47 0.28663616638880995 1102 48-49 0.20430989474866124 1102 50-51 -0.010669702342127607 1102 52-53 0.22783667217802162 1102 54-55 0.28939114339734573 1102 56-57 0.03693199304775874 1102 58-59 -0.0018361674544138396 1102 60-61 0.05700968035336729 1102 62-63 0.10564808762639899 1102 64-65 -0.16252944385138335 1102 66-67 -0.14296936627074075 1102 68-69 -0.18386612517401346 1102 70-71 -0.01088619787061873 1102 72-73 -0.07894480106448043 1102 74-75 -0.18389704765568382 1102 76-77 -0.17212652836412445 1102 78-79 -0.03727923942012268 1102 80-81 -0.05775635668896939 1102 82-83 -0.13775159976253093 1102 84-85 -0.05615543412108792 1102 86-87 -0.10390902071618058 1102 88-89 -0.20640178647487772 1102 90-91 -0.1397507912066729 1102 92-93 -0.00920537584402581 1102 94-95 -0.028969682871334612 1102 96-97 -0.134084856277898 1102 98-99 0.08809292410029457 1102 100-101 -0.0497088103256651 1103 1 0.39502993362071237 1103 2 0.18075835581906574 1103 3 0.262407303763041 1103 4 0.2483608061567324 1103 5 0.24020136666291592 1103 6 0.1750749110670995 1103 7 0.18166341419612309 1103 8 0.138478002306762 1103 9 0.18766231979826387 1103 10-11 0.28166425045210985 1103 12-13 0.250352349428411 1103 14-15 0.2960626853486943 1103 16-17 0.37960119321623864 1103 18-19 0.2366201909161063 1103 20-21 0.34337037085176547 1103 22-23 0.3348030413747054 1103 24-25 0.24800079046399048 1103 26-27 0.38035996154628293 1103 28-29 0.3216631310849323 1103 30-31 0.3334501606622382 1103 32-33 0.4483995829026455 1103 34-35 0.28127498758254177 1103 36-37 0.18289684580157228 1103 38-39 0.3643120492152434 1103 40-41 0.211821659135083 1103 42-43 0.2476884775191266 1103 44-45 0.2891392998298059 1103 46-47 0.32262944354059897 1103 48-49 0.4215912443683365 1103 50-51 0.40550379764209055 1103 52-53 0.4407663189917699 1103 54-55 0.24308476458220696 1103 56-57 0.2774158487432459 1103 58-59 0.2421393083528045 1103 60-61 0.32226233950812144 1103 62-63 0.3842241378741633 1103 64-65 0.3851972550092029 1103 66-67 0.3688981967756817 1103 68-69 0.4178919154155736 1103 70-71 0.548909276042842 1103 72-73 0.5417113867965213 1103 74-75 0.32006801247119654 1103 76-77 0.2901284664280297 1103 78-79 0.28723232829758416 1103 80-81 0.3004483587735862 1103 82-83 0.328937949838199 1103 84-85 0.43387076289308624 1103 86-87 0.4324748444481017 1103 88-89 0.5548621405522312 1103 90-91 0.3997025433891608 1103 92-93 0.5116970008248529 1103 94-95 0.4294938464661655 1103 96-97 0.41956378810552764 1103 98-99 0.3553419219855982 1103 100-101 0.3391905994524542 1104 1 0.23507639654852852 1104 2 0.10187499303739855 1104 3 0.14452125791089543 1104 4 0.19344511564781897 1104 5 0.17204483240468704 1104 6 0.15635340505745177 1104 7 0.20095207331529252 1104 8 0.21285140718864426 1104 9 0.23437065040490523 1104 10-11 0.24181629111262026 1104 12-13 0.29249073478713683 1104 14-15 0.2384393840813246 1104 16-17 0.305110540335761 1104 18-19 0.15341501237194421 1104 20-21 0.2972155598664443 1104 22-23 0.18521368203958133 1104 24-25 0.17483531263749796 1104 26-27 0.2028873921245662 1104 28-29 0.1899581815833713 1104 30-31 0.19744248228167294 1104 32-33 0.11550797458730955 1104 34-35 0.17388127425662248 1104 36-37 0.14190912684775725 1104 38-39 0.18512166027908705 1104 40-41 0.07354809501406123 1104 42-43 0.14576179296532388 1104 44-45 0.11471517837178169 1104 46-47 0.1888276635523809 1104 48-49 0.19538986138729086 1104 50-51 0.12539832897730463 1104 52-53 0.2693415649399 1104 54-55 0.1826311511624894 1104 56-57 0.18091256579928228 1104 58-59 0.12343525320807203 1104 60-61 0.42336556137405523 1104 62-63 0.4922886188200053 1104 64-65 0.3798256606545536 1104 66-67 0.1981605705295877 1104 68-69 0.16479438689432158 1104 70-71 0.25303804161227816 1104 72-73 0.4556595359141653 1104 74-75 0.17940040782054467 1104 76-77 0.16114748787078526 1104 78-79 0.25889103300713856 1104 80-81 0.2593052180541733 1104 82-83 0.273945911795316 1104 84-85 0.3858890644876638 1104 86-87 0.1696839058096984 1104 88-89 0.2878121001971081 1104 90-91 0.24588134931372707 1104 92-93 0.15509130311745878 1104 94-95 0.19285739160937254 1104 96-97 0.1730294208084473 1104 98-99 0.248010922705447 1104 100-101 0.2806020472198334 1105 1 0.23858333455328307 1105 2 0.23328962663543962 1105 3 0.1671084115024115 1105 4 0.23359505972789663 1105 5 0.22296849468206403 1105 6 0.17525377766961725 1105 7 0.20255379980785193 1105 8 0.21445313368120367 1105 9 0.29670310138364187 1105 10-11 0.35873108488330985 1105 12-13 0.19331857447960488 1105 14-15 0.3303588144287062 1105 16-17 0.3958441310693459 1105 18-19 0.35063531439573836 1105 20-21 0.4416575689372735 1105 22-23 0.29873674971111797 1105 24-25 0.22204971356369185 1105 26-27 0.3276590758482101 1105 28-29 0.3695932127467856 1105 30-31 0.3362503481637731 1105 32-33 0.4069210345593106 1105 34-35 0.35814445196957934 1105 36-37 0.4525906755205682 1105 38-39 0.5087406629373916 1105 40-41 0.43690677496663 1105 42-43 0.4608635447482996 1105 44-45 0.45594474230876614 1105 46-47 0.5203541370002469 1105 48-49 0.437337673204091 1105 50-51 0.6387797703063356 1105 52-53 0.7019763207187637 1105 54-55 0.521694169264812 1105 56-57 0.5006537043177133 1105 58-59 0.7190161741164971 1105 60-61 0.6620256188114055 1105 62-63 0.608054103581118 1105 64-65 0.582635145123426 1105 66-67 0.4404849285744703 1105 68-69 0.29417173679568265 1105 70-71 0.37352440445672386 1105 72-73 0.39836830831725933 1105 74-75 0.46164709618076927 1105 76-77 0.42094471491691365 1105 78-79 0.517100583793102 1105 80-81 0.5502602404502426 1105 82-83 0.45096197964107176 1105 84-85 0.5330399583634389 1105 86-87 0.47670115387499123 1105 88-89 0.657639507142413 1105 90-91 0.6180832455330929 1105 92-93 0.5193913486402124 1105 94-95 0.4037401396100222 1105 96-97 0.5223041478124273 1105 98-99 0.28954059960629763 1105 100-101 0.517227631006989 1106 1 0.16931305908708083 1106 2 0.040672011079699644 1106 3 0.12703261023357015 1106 4 0.17669467248228443 1106 5 0.14915563593059034 1106 6 0.13849565512423112 1106 7 0.10256203907620431 1106 8 0.12326640439609804 1106 9 0.10510715510367419 1106 10-11 0.03720736952256942 1106 12-13 0.13944199871953344 1106 14-15 0.11425104479864956 1106 16-17 0.11093847069199114 1106 18-19 0.007505490990723729 1106 20-21 0.14495602270124408 1106 22-23 -0.023588921102643212 1106 24-25 0.12680102498056556 1106 26-27 0.029499179113706475 1106 28-29 0.15123886927915464 1106 30-31 0.10527619200217941 1106 32-33 0.0067782205745317015 1106 34-35 0.10329289612106862 1106 36-37 -0.024490426344250693 1106 38-39 -0.006029598796693847 1106 40-41 -0.20585502778528308 1106 42-43 -0.056999189040936926 1106 44-45 -0.11243569217531757 1106 46-47 -0.3142562829277935 1106 48-49 -0.14466501829023315 1106 50-51 -0.21238608616560128 1106 52-53 -0.16895279410917396 1106 54-55 -0.09936540064537525 1106 56-57 -0.15610936178868684 1106 58-59 -0.11371294620428074 1106 60-61 -0.08215883094278809 1106 62-63 -0.10973173037016437 1106 64-65 -0.07887373955428245 1106 66-67 -0.07202228078534034 1106 68-69 0.01211960969123993 1106 70-71 0.09611373185778405 1106 72-73 0.10304546631896017 1106 74-75 0.10458192356809093 1106 76-77 0.2473158664919879 1106 78-79 0.20571116850651805 1106 80-81 0.1661263248752789 1106 82-83 0.20196854359152638 1106 84-85 0.12573996660271547 1106 86-87 0.24404344054659788 1106 88-89 0.16804570307221667 1106 90-91 0.13073601530047796 1106 92-93 0.16357579839440461 1106 94-95 0.05125325377881751 1106 96-97 0.17618621641806698 1106 98-99 0.06737422131402582 1106 100-101 0.36964310984579996 1107 1 0.07067236117675435 1107 2 0.11678391117212072 1107 3 0.1074703821503391 1107 4 0.10417151024518034 1107 5 0.04461786626804809 1107 6 -0.014084313767362744 1107 7 -0.01258865635446682 1107 8 -0.029363157606567825 1107 9 0.027025055962532463 1107 10-11 0.0543846198099871 1107 12-13 0.07316080577979278 1107 14-15 0.18535404067340266 1107 16-17 0.16585607850065287 1107 18-19 0.1066285946177814 1107 20-21 0.06226401846328855 1107 22-23 0.11871400263667198 1107 24-25 -0.030609763775323984 1107 26-27 0.050583014013732 1107 28-29 0.041683178469661186 1107 30-31 -0.04677177806161836 1107 32-33 0.07731540413666949 1107 34-35 0.08878465690194304 1107 36-37 0.08022993613630547 1107 38-39 -0.06324652357296401 1107 40-41 0.04656489061150637 1107 42-43 0.015156533327804311 1107 44-45 0.03890895740426714 1107 46-47 0.1505889640233562 1107 48-49 -0.029196476102278268 1107 50-51 0.010947472590963514 1107 52-53 0.10070749308002291 1107 54-55 -0.04192981533395823 1107 56-57 0.084009210831411 1107 58-59 0.021552264228304807 1107 60-61 0.07990920178865935 1107 62-63 0.15454995850069508 1107 64-65 0.001426524190904388 1107 66-67 0.15337615477742617 1107 68-69 -0.08102977828468738 1107 70-71 -0.07772590205488683 1107 72-73 0.1608439830340913 1107 74-75 0.0735433987377263 1107 76-77 -0.1815292902898662 1107 78-79 0.21059158148187862 1107 80-81 -0.060396972615762934 1107 82-83 0.056415016845065225 1107 84-85 0.2000327012119456 1107 86-87 0.0972658656497245 1107 88-89 -0.014116556570257899 1107 90-91 -0.16646648238894102 1107 92-93 -0.16193309455210425 1107 94-95 -0.03444364214327322 1107 96-97 0.03288466776046306 1107 98-99 0.004059598108959506 1107 100-101 -0.06871039886680919 1108 1 0.06925286438836764 1108 2 0.010404456022307329 1108 3 0.027221497046774346 1108 4 0.08784729717871187 1108 5 0.019741185051536547 1108 6 0.06125547827635813 1108 7 0.022058894422116282 1108 8 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-0.3327298884288794 2316 84-85 -0.3691343921487018 2316 86-87 -0.48785958237894533 2316 88-89 -0.42109727000258346 2316 90-91 -0.38962743135633815 2316 92-93 -0.3471609491347962 2316 94-95 -0.22952812755033847 2316 96-97 -0.3996550147792135 2316 98-99 -0.439403450192799 2316 100-101 -0.17986417937542853 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 0.0 12 3.0 13 9.0 14 34.0 15 133.0 16 296.0 17 673.0 18 1128.0 19 1539.0 20 2310.0 21 3282.0 22 3946.0 23 4029.0 24 4651.0 25 6127.0 26 8910.0 27 12253.0 28 16282.0 29 15991.0 30 12256.0 31 11931.0 32 14719.0 33 19105.0 34 26285.0 35 38840.0 36 69356.0 37 166451.0 38 301442.0 39 118240.0 40 40.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.17255190101227 21.176264057190224 12.87640368448649 25.774780357311016 2 11.942130919949403 23.65245037058415 43.89487489512044 20.51054381434601 3 16.24156361666562 31.649892707105508 29.9106551259965 22.19788855023237 4 9.537675731346962 21.535532198330277 41.712176057991634 27.214616012331128 5 12.351585912198841 38.86420648593103 34.862402384389874 13.921805217480257 6 23.048549264780245 42.82316479873148 21.486970178524306 12.64131575796396 7 26.25159297880059 31.093899705124507 23.777824804889168 18.876682511185734 8 23.187819524749436 37.11811052911788 24.925894669298422 14.768175276834267 9 26.945511948685404 14.47407882714801 19.068493086989776 39.51191613717681 10-11 21.63817534658046 28.432675161753046 30.84031376487628 19.088835726790208 12-13 24.19919745379896 25.92192843575562 28.891837602962816 20.987036507482603 14-15 22.675998707370546 26.149707879692464 25.98132894397288 25.192964468964107 16-17 20.988140822214625 30.381441932806517 26.93092336985709 21.699493875121764 18-19 21.251200215748227 28.676496230218234 27.91783200931809 22.15447154471545 20-21 22.075483980461765 27.667443174288763 27.258207383332056 22.99886546191742 22-23 21.80841418079608 29.197267809109317 27.455298502084247 21.53901950801035 24-25 21.757208850326993 31.238797017652765 26.3486588969407 20.655335235079544 26-27 21.42103219716784 31.18950970750771 27.231006367827476 20.158451727496974 28-29 20.826830851782944 28.97488342031401 28.462705834418777 21.735579893484267 30-31 22.786953277024907 29.298922886283478 27.51969748750962 20.394426349181995 32-33 21.633060625716354 28.770595469752237 28.716309682399082 20.880034222132327 34-35 21.234635494767872 28.712473641751004 28.776465774380362 21.276425089100762 36-37 20.72033952486513 29.66553173978595 27.674566207232452 21.939562528116465 38-39 20.617614168706744 27.324640632737466 29.68642111356773 22.37132408498806 40-41 20.178968732781406 26.986545959254272 31.32789777997866 21.50658752798566 42-43 21.704201743189866 26.2639753935429 30.13895766638536 21.892865196881882 44-45 19.715621519955548 27.809725409235792 29.89048685168007 22.58416621912859 46-47 21.92971443583466 26.40218910052984 28.792681764392707 22.87541469924279 48-49 20.947177108356005 26.43876309703503 28.991707753979455 23.622352040629508 50-51 19.506266695495096 26.881171085088027 29.921814516972738 23.69074770244414 52-53 19.509056543239154 27.01886169562296 31.781073672904302 21.691008088233584 54-55 19.76740806870465 27.41153276559932 29.95250284215739 22.868556323538645 56-57 20.92664792818932 27.83035865817623 28.091325665901785 23.151667747732667 58-59 18.603692828463885 28.27778049012975 30.598585082219138 22.519941599187224 60-61 20.709272291464693 30.340407922237645 27.680113732793032 21.27020605350463 62-63 19.178852904935717 31.38469764954781 27.653151372840767 21.783298072675702 64-65 18.982702312499892 31.326012674082648 27.29042806269504 22.400856950722428 66-67 19.302026591898745 31.86924448598218 26.854958140659473 21.9737707814596 68-69 19.850812891886424 30.33122467341345 27.461285050368378 22.35667738433175 70-71 20.107362640683885 30.1926041136305 26.880996719604028 22.81903652608159 72-73 19.73079131706387 30.38591731356261 26.7714951956497 23.11179617372382 74-75 20.117708326068104 29.768082281909464 26.343369810592588 23.770839581429843 76-77 19.814719236697655 29.683863753135675 27.578807386586877 22.922609623579792 78-79 20.352288023881098 28.86649648595428 26.976084030214054 23.805131459950573 80-81 19.78751822119308 30.183130255666295 26.359469556948927 23.669881966191696 82-83 20.342814165916895 29.75430740867317 26.213235037697817 23.689643387712113 84-85 20.727580667285082 28.503874401054563 26.17667640788504 24.591868523775315 86-87 19.51783293926734 29.174658418016836 27.371021851482457 23.93648679123337 88-89 20.286784723723702 28.371937851491758 27.45332235993221 23.88795506485234 90-91 20.69020833188029 28.123641402270472 26.975270324622038 24.210879941227205 92-93 20.524677365732767 28.219658662128516 26.701923367532217 24.553740604606503 94-95 20.188675078057617 27.429085557655693 27.16242261078602 25.21981675350068 96-97 20.600468229446378 27.21054748437104 27.20688580920696 24.98209847697562 98-99 20.202775787101697 26.989615354883785 27.16043570523115 25.64717315278337 100-101 20.071746460192163 27.799544668041847 27.582456990775373 24.546251880990617 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 24.0 1 15.0 2 28.5 3 48.5 4 34.0 5 28.0 6 47.5 7 116.0 8 181.5 9 177.5 10 139.5 11 110.5 12 115.0 13 146.5 14 165.0 15 155.5 16 157.5 17 177.0 18 203.5 19 233.0 20 245.0 21 271.0 22 311.5 23 334.5 24 386.5 25 465.0 26 561.5 27 661.5 28 763.0 29 902.0 30 1119.0 31 1553.5 32 2921.5 33 5910.0 34 10011.5 35 18042.5 36 36967.0 37 56674.5 38 64522.0 39 64304.0 40 57124.5 41 50436.5 42 48293.0 43 45713.5 44 43719.5 45 41619.5 46 39401.0 47 36935.5 48 34627.0 49 32318.5 50 28248.5 51 21871.0 52 15640.5 53 12644.5 54 11331.0 55 10511.0 56 9657.0 57 8657.5 58 8095.0 59 7250.5 60 5918.5 61 4716.0 62 4017.5 63 3489.5 64 2503.5 65 1881.5 66 1436.0 67 850.0 68 576.5 69 433.5 70 341.5 71 273.5 72 192.5 73 140.0 74 73.5 75 38.5 76 26.0 77 18.5 78 12.0 79 3.5 80 2.0 81 0.5 82 0.0 83 0.5 84 2.0 85 1.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013368020440284473 2 0.10961776761033266 3 0.0 4 0.0 5 0.0 6 0.0049984772081063675 7 0.027665990128588735 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0015692893560333946 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.1624365600247367E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 5.812182800123684E-5 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 2.3248731200494735E-4 64-65 2.9060914000618416E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.7436548400371048E-4 100-101 0.0012205583880259734 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 860262.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.500250848649866 #Duplication Level Percentage of deduplicated Percentage of total 1 64.80670381289691 13.933603886514373 2 13.03427629344512 5.604804198793606 3 5.701046144265698 3.6772176660432185 4 3.357614259553199 2.887581953335902 5 2.247668736534694 2.4162720830081916 6 1.6024062874002802 2.0671282284335857 7 1.2531331736168776 1.8859874305669302 8 0.9803511312324825 1.686223619300483 9 0.7131364603346061 1.3799351507861048 >10 5.264779436636138 22.180488878483622 >50 0.5698004747841848 8.561240730552287 >100 0.4073183081464487 17.564182142729283 >500 0.04562855364482076 6.741558344977216 >1k 0.015024035956221472 5.90720824918536 >5k 5.564457761563508E-4 1.1852461139962607 >10k+ 5.564457761563508E-4 2.3213213232935743 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 19403 2.2554756574159964 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 9907 1.1516259000165068 RNA PCR Primer, Index 33 (95% over 22bp) ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 4500 0.5230964520111314 RNA PCR Primer, Index 33 (95% over 24bp) CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 3917 0.4553264005616894 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 2927 0.3402451811192404 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 2799 0.3253659931509238 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 2516 0.2924690385022237 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 2377 0.2763111703178799 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 2316 0.26922030730172897 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 2067 0.24027563695711304 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1944 0.22597766726880883 No Hit GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT 1767 0.20540254015637094 No Hit TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG 1669 0.19401066186812854 RNA PCR Primer, Index 33 (96% over 29bp) TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA 1510 0.17552792056373523 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1506 0.17506294593972535 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 1484 0.17250558550767092 No Hit GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG 1456 0.16925076313960166 No Hit GTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATT 1443 0.1677395956115695 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 1418 0.16483350421150766 No Hit CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG 1365 0.15867259044337656 No Hit CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT 1320 0.15344162592326524 RNA PCR Primer, Index 33 (96% over 27bp) TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC 1297 0.15076802183520832 No Hit CCATGTAATTATTGGATCAACATTCCTTATTGTTTGCCTACTACGACAAC 1263 0.14681573753112426 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 1131 0.13147157493879771 No Hit TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT 1104 0.12833299622673092 RNA PCR Primer, Index 33 (95% over 21bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1097 0.12751929063471362 No Hit TTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACATCATTCTCCA 1084 0.12600812310668144 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1063 0.1235670063306295 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 1036 0.12042842761856272 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 1000 0.11624365600247366 No Hit GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA 989 0.11496497578644645 No Hit GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA 970 0.11275634632239946 No Hit CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC 962 0.11182639707437968 No Hit CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT 950 0.11043147320234999 No Hit CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG 933 0.10845533105030793 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 916 0.10647918889826588 No Hit GTGATGTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAA 893 0.10380558481020899 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC 884 0.10275939190618673 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 877 0.10194568631416939 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 868 0.10089949341014713 No Hit CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC 867 0.10078324975414465 No Hit CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC 867 0.10078324975414465 No Hit TGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAA 865 0.10055076244213973 No Hit CTTTGAAACATCATTCTCCATTTCAGATGGTATCTATGGTTCTACATTCT 865 0.10055076244213973 No Hit CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG 861 0.10008578781812982 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.3248731200494732E-4 0.0 5 0.0 0.0 0.0 3.4873096800742097E-4 0.0 6 0.0 0.0 0.0 3.4873096800742097E-4 0.0 7 0.0 0.0 0.0 3.4873096800742097E-4 0.0 8 0.0 0.0 0.0 3.4873096800742097E-4 0.0 9 0.0 0.0 0.0 5.812182800123683E-4 0.0 10-11 0.0 0.0 0.0 0.0012205583880259734 0.0 12-13 0.0 0.0 0.0 0.0015692893560333944 0.0 14-15 0.0 0.0 0.0 0.0019761421520420523 0.0 16-17 0.0 0.0 0.0 0.0029642132280630782 0.0 18-19 0.0 0.0 0.0 0.0035454315080754467 0.0 20-21 0.0 0.0 0.0 0.0049984772081063675 0.0 22-23 0.0 0.0 0.0 0.011101269148236234 0.0 24-25 0.0 0.0 0.0 0.020633248940439074 0.0 26-27 0.0 0.0 0.0 0.04330076186092144 0.0 28-29 0.0 0.0 0.0 0.07567462005761036 0.0 30-31 0.0 0.0 0.0 0.1664609153955423 0.0 32-33 0.0 0.0 0.0 0.3390827445592157 0.0 34-35 0.0 0.0 0.0 0.5742436606522199 0.0 36-37 0.0 0.0 0.0 0.8873459480948828 0.0 38-39 0.0 0.0 0.0 1.4149759026901108 0.0 40-41 0.0 0.0 0.0 2.0883754019124403 0.0 42-43 0.0 0.0 0.0 2.667850027084772 0.0 44-45 0.0 0.0 0.0 3.241919322252988 0.0 46-47 0.0 0.0 0.0 3.8986959786669644 0.0 48-49 0.0 0.0 0.0 4.57750080789341 0.0 50-51 0.0 0.0 0.0 5.286122134884488 0.0 52-53 0.0 0.0 0.0 5.9879432079994235 0.0 54-55 0.0 0.0 0.0 6.650648290869526 0.0 56-57 0.0 0.0 0.0 7.336079008488111 0.0 58-59 0.0 0.0 0.0 8.086083077016072 0.0 60-61 0.0 0.0 0.0 8.856778516312472 0.0 62-63 0.0 0.0 0.0 9.588881061816052 0.0 64-65 0.0 0.0 0.0 10.350335130460255 0.0 66-67 0.0 0.0 0.0 11.151777016769309 0.0 68-69 0.0 0.0 0.0 11.976351390622856 0.0 70-71 0.0 0.0 0.0 12.902406476166563 0.0 72-73 0.0 0.0 0.0 13.828170952570265 0.0 74-75 0.0 0.0 0.0 14.741787966921706 0.0 76-77 0.0 0.0 0.0 15.619543813396383 0.0 78-79 0.0 0.0 0.0 16.588260320692996 0.0 80-81 0.0 0.0 0.0 17.640730382139395 0.0 82-83 0.0 0.0 0.0 18.691398666917756 0.0 84-85 0.0 0.0 0.0 19.780834210972934 0.0 86-87 0.0 0.0 0.0 20.855274323403798 0.0 88-89 0.0 0.0 0.0 21.968132964143482 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTATCG 20 0.002074236 71.24708 9 CCGTTTA 25 0.0049985955 57.064 2 GACATAT 115 1.8189894E-12 45.49374 1 GGTATCA 1825 0.0 43.261543 1 GTATTGG 320 0.0 43.10273 1 GTATCAA 3450 0.0 41.220085 1 CATATAG 105 1.024091E-9 40.724453 3 GTCATAA 50 0.0016053298 38.04931 1 GTTCGCA 50 0.0016159153 37.998444 9 CGCAGTG 50 0.0016159153 37.998444 8 ATGGGCG 80 1.07545075E-5 35.633896 5 ATTAGCC 175 0.0 35.294525 3 GTCCACG 55 0.0025761104 34.55408 6 TGTTCGC 55 0.0025797852 34.544037 8 TTAGACG 35 2.05425E-4 33.92718 90-91 ATTATGC 85 1.6289414E-5 33.537785 3 TCAACGC 4320 0.0 32.004517 4 GGGATCG 60 0.003946702 31.672731 7 CATCGTA 30 0.003952286 31.665367 38-39 AACGCAG 4370 0.0 31.638332 6 >>END_MODULE