FastQCFastQC Report
Fri 10 Feb 2017
SRR2568626_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568626_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1603342
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25930.16172469753801746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19670.12268124954002328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20450.051.8196221
GTATCAA37950.043.07541
TCAACGC47050.034.5421334
ATCAACG47300.034.3595623
CAACGCA48600.033.4394345
AACGCAG49050.033.132656
TATCAAC50950.031.7115462
ACGCAGA57150.028.0209437
CGCAGAG58550.027.4320898
GTACATG59700.026.6657091
TATACCG1351.3389574E-524.6395845
TACATGG62200.024.6007392
ACATGGG59950.024.4142953
GCAGAGT67850.022.8301989
GTATAGG7150.022.5972481
AGAGTAC62000.020.34767310-11
CATGGGA21750.019.8822174
TATAGCG1456.1294856E-419.663732
CATGGGG41800.019.440284
CAGAGTA66700.018.80702210-11
Produced by FastQC (version 0.11.5)