FastQCFastQC Report
Fri 10 Feb 2017
SRR2568626_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568626_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1603342
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29620.18473912615025365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24580.15330478463110178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18700.053.8771
GTATCAA38700.044.3307761
TCAACGC45550.037.0184974
ATCAACG46700.036.113663
CAACGCA47300.035.849735
AACGCAG48750.034.8775986
TATCAAC50550.033.369412
ACGCAGA55650.030.2971217
CGCAGAG57050.029.553638
TACCGCG650.005848429.2270378
GTACATG58150.025.2532861
GCAGAGT67550.024.8894949
ACATGGG59850.023.2575263
TACATGG63100.022.8920962
GTATAGG7550.022.0307961
AGAGTAC62400.020.66442710-11
GAGTACT33100.020.15981112-13
GACCGGT1656.330927E-520.1489417
CATGGGG38000.019.2493444
CAGAGTA67050.019.08965710-11
Produced by FastQC (version 0.11.5)