FastQCFastQC Report
Fri 10 Feb 2017
SRR2568625_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568625_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1735255
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18560.10695834329824724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37500.048.91791
GGTATCA18900.044.7579271
TCAACGC42250.042.2922064
CAACGCA42600.041.8307655
ATCAACG43350.041.110613
AACGCAG45050.039.659036
TATCAAC46850.038.3436972
ACGCAGA53300.033.2529877
CGCAGAG53500.032.8612758
GCAGAGT62550.027.1944089
GTACATG60050.024.2961311
TACATGG60200.022.9724982
ACATGGG60800.022.0423183
GTATTAT7200.021.7817731
AGAGTAC55600.020.93703810-11
TACCGTC1200.004754602419.7987527
CAGAGTA61100.019.63559710-11
CATGGGG41000.018.5453624
GAGTACT28450.017.87003712-13
ATAGGCG1600.001188029117.8199045
Produced by FastQC (version 0.11.5)