FastQCFastQC Report
Fri 10 Feb 2017
SRR2568625_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568625_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1735255
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23170.133525043869633No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18460.10638205912099374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20000.048.2400861
GTATCAA38050.043.3427851
TCAACGC43050.037.074074
CAACGCA43700.036.5226255
ATCAACG44200.036.11783
AACGCAG45550.035.0352336
TATCAAC46400.034.8219342
ACGCAGA54500.029.1074457
CGCAGAG56000.028.158158
GCAGAGT65600.023.4582319
GTACATG58700.022.913441
TACATGG57950.022.551282
TACATCG1805.17017E-621.1116245
ACATGGG59350.020.894123
AGAGTAC57250.019.74492810-11
CAGAGTA62500.019.15025710-11
GAGTACT31700.018.0543512-13
GTGGTAT14000.017.313471
CATGGGG40400.016.9311054
GTCCTAT4651.2732926E-1116.3534361
Produced by FastQC (version 0.11.5)