FastQCFastQC Report
Fri 10 Feb 2017
SRR2568623_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568623_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2560489
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24750.043.5936551
GTATCAA42100.037.3696061
TCAACGC45700.034.4251564
ATCAACG46800.033.6166763
CAACGCA47350.033.0234955
AACGCAG49200.031.779896
GTACATG90400.029.706581
TATCAAC53950.028.897152
ACGCAGA56900.027.3957487
ACATGGG94000.026.6979983
TACATGG97150.026.5662142
CGCAGAG58450.026.4248148
CATGGGG58450.023.5818354
GCAGAGT73500.020.5614079
GTATAGG10350.019.2877481
CATGGGA38350.018.9622634
AGAGTAC63250.018.25823610-11
TATACCG2352.5158097E-618.202115
TAATACT18400.017.8235974
CAGAGTA67750.017.711910-11
Produced by FastQC (version 0.11.5)