FastQCFastQC Report
Fri 10 Feb 2017
SRR2568623_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568623_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2560489
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23250.044.7603841
GTATCAA42700.040.842361
TCAACGC48200.035.083064
ATCAACG48850.034.6216553
CAACGCA49650.034.058485
AACGCAG50950.033.1849336
TATCAAC55650.030.4812852
GTACATG94400.028.390941
ACGCAGA59600.028.0499137
CGCAGAG60950.027.194868
ACATGGG96600.025.9668433
TACATGG101600.025.7216742
CATGGGG58450.024.1360634
GTATTAC5350.021.3171981
GCAGAGT77350.021.3061169
TACCGTA2401.4168836E-719.7890017
AGAGTAC66300.018.62494310-11
GTATATA13100.017.7745021
CAGAGTA72000.017.41432210-11
TACACCG2606.6377324E-616.4400947
Produced by FastQC (version 0.11.5)