FastQCFastQC Report
Fri 10 Feb 2017
SRR2568621_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568621_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2284321
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22600.044.1596071
GTATCAA43050.043.0532341
TCAACGC45150.038.734234
ATCAACG46450.037.8556183
CAACGCA47650.036.9006585
AACGCAG49500.035.5176436
TATCAAC52700.033.9071732
CGCAGAG57700.030.3060388
ACGCAGA57900.029.9558077
GTACATG78450.025.3219811
TACATGG77000.024.502712
GCAGAGT70900.024.1951149
ACATGGG77400.023.455073
CATGGGG44750.022.0889534
AGAGTAC61650.019.53941510-11
CAGTGCG3851.2732926E-1118.5138729
GTATATA20650.018.4112911
CAGAGTA69850.018.33407810-11
GTATAGG8700.016.9338931
GAGTACT28150.016.79621512-13
Produced by FastQC (version 0.11.5)