FastQCFastQC Report
Fri 10 Feb 2017
SRR2568621_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568621_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2284321
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23700.043.105181
GTATCAA42900.040.6488381
TCAACGC47100.035.5984844
CAACGCA48400.034.7404635
ATCAACG48850.034.5259863
AACGCAG51100.032.9941986
TATCAAC52850.032.183952
ACGCAGA60900.027.6068047
CGCAGAG61000.027.1722588
GTACATG79250.022.543841
GCAGAGT73000.022.3152339
TACATGG78200.022.236972
ACATGGG76950.021.9180563
CATGGGG49500.019.575044
AGAGTAC64700.019.2321210-11
GTATAAT15050.018.6275121
CAGAGTA70750.017.72179210-11
GTATAAG12000.017.0265461
GAGTACT29850.016.14923312-13
GTATAGG9150.016.0982971
Produced by FastQC (version 0.11.5)