##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568620_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 61080 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.70841519318926 34.0 31.0 34.0 31.0 34.0 2 32.725589390962675 34.0 31.0 34.0 31.0 34.0 3 32.76776358873608 34.0 31.0 34.0 31.0 34.0 4 36.169482645710545 37.0 35.0 37.0 35.0 37.0 5 36.18272757039948 37.0 35.0 37.0 35.0 37.0 6 36.3687459070072 37.0 37.0 37.0 35.0 37.0 7 36.297429600523905 37.0 37.0 37.0 35.0 37.0 8 36.32899476096922 37.0 37.0 37.0 35.0 37.0 9 38.06057629338572 39.0 39.0 39.0 37.0 39.0 10-11 38.08218729535036 39.0 39.0 39.0 36.0 39.0 12-13 38.04764243614932 39.0 39.0 39.0 35.0 39.0 14-15 39.20299607072691 41.0 39.0 41.0 36.0 41.0 16-17 39.20694990176817 40.5 38.5 41.0 36.0 41.0 18-19 39.4445317616241 41.0 39.0 41.0 36.0 41.0 20-21 39.513482318271116 41.0 39.0 41.0 37.0 41.0 22-23 39.45063032089064 41.0 39.0 41.0 36.0 41.0 24-25 39.28166339227243 41.0 39.0 41.0 35.0 41.0 26-27 38.99616895874263 41.0 38.5 41.0 35.0 41.0 28-29 38.834954158480684 40.0 38.0 41.0 35.0 41.0 30-31 38.5509986902423 40.0 37.5 41.0 35.0 41.0 32-33 37.877627701375246 40.0 35.5 41.0 34.0 41.0 34-35 37.48402914210871 40.0 35.0 41.0 33.0 41.0 36-37 37.27319089718402 40.0 35.0 41.0 33.0 41.0 38-39 36.59827275703995 39.0 35.0 41.0 32.0 41.0 40-41 36.44440078585462 39.0 35.0 41.0 31.0 41.0 42-43 35.9768909626719 39.5 35.0 41.0 28.5 41.0 44-45 35.242706286836935 39.0 35.0 41.0 23.5 41.0 46-47 34.44045514079895 38.5 35.0 41.0 16.5 41.0 48-49 33.62158644400786 38.0 33.5 41.0 9.0 41.0 50-51 32.48853143418468 38.0 32.0 40.0 7.5 41.0 52-53 32.5842665356909 38.0 32.5 40.0 7.0 41.0 54-55 31.836714145383105 37.0 31.0 40.0 7.0 41.0 56-57 30.997986247544205 36.5 28.5 39.5 7.0 41.0 58-59 30.441691224623447 36.0 24.5 39.0 7.0 41.0 60-61 29.28912082514735 35.0 19.5 39.0 7.0 41.0 62-63 28.326890962671904 35.0 15.0 39.0 7.0 41.0 64-65 27.289603798297314 35.0 11.0 38.5 7.0 40.0 66-67 25.691404715127703 32.0 10.0 37.0 7.0 39.0 68-69 23.92401768172888 27.0 8.0 37.0 7.0 39.0 70-71 22.77964145383104 24.0 7.5 35.5 7.0 38.0 72-73 22.13397184020956 23.5 7.0 35.0 7.0 37.0 74-75 21.81476751800917 23.0 7.0 35.0 7.0 37.0 76-77 20.75264407334643 20.0 7.0 33.5 7.0 35.0 78-79 21.43160609037328 23.0 7.0 35.0 7.0 36.0 80-81 21.39188768827767 23.0 7.0 35.0 7.0 35.5 82-83 21.241928618205634 21.5 7.0 35.0 7.0 35.0 84-85 21.032326457105434 20.0 7.0 35.0 7.0 35.0 86-87 21.049066797642435 20.0 7.0 35.0 7.0 35.0 88-89 20.9318107400131 20.0 7.0 35.0 7.0 35.0 90-91 20.78965291421087 20.0 7.0 35.0 7.0 35.0 92-93 20.769720039292732 19.5 7.0 35.0 7.0 35.0 94-95 20.7641781270465 20.0 7.0 35.0 7.0 35.0 96-97 20.721422724296005 20.0 7.0 35.0 7.0 35.0 98-99 20.801457105435496 20.0 7.0 35.0 7.0 35.0 100-101 20.21990013097577 19.5 7.0 34.5 6.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.44675126014510624 1101 2 0.2817806370060154 1101 3 0.33368785715163796 1101 4 0.23263527606965084 1101 5 0.03479620960488461 1101 6 0.21068099976219656 1101 7 0.30172640970440057 1101 8 0.4632485699332136 1101 9 0.7687171774816832 1101 10-11 0.5066105917489878 1101 12-13 0.5721527158498247 1101 14-15 0.7800254875749317 1101 16-17 0.6697635377723543 1101 18-19 0.17750716993460713 1101 20-21 0.784133524431752 1101 22-23 1.0357750963508465 1101 24-25 1.0145507859928458 1101 26-27 0.9125578545477637 1101 28-29 0.6483546300192486 1101 30-31 0.9701484032652985 1101 32-33 0.8242112023933714 1101 34-35 0.7809785110799154 1101 36-37 0.9390160887476497 1101 38-39 1.473511273750752 1101 40-41 1.890263814249515 1101 42-43 2.2897447326081846 1101 44-45 1.3642595754207179 1101 46-47 1.5693421237007428 1101 48-49 0.45417791822266196 1101 50-51 0.2690092314221175 1101 52-53 -0.1139851120435722 1101 54-55 0.6553828540484403 1101 56-57 1.3034769376960398 1101 58-59 1.0081250207795556 1101 60-61 2.234373583072639 1101 62-63 2.726826936567825 1101 64-65 2.4240086814853825 1101 66-67 3.2901416255954636 1101 68-69 2.6987550480126465 1101 70-71 2.172981093339306 1101 72-73 2.1209062722216494 1101 74-75 2.6254323900445833 1101 76-77 2.7499837671228455 1101 78-79 2.720756953095151 1101 80-81 1.490164580674982 1101 82-83 1.094647453036469 1101 84-85 1.3098499539667579 1101 86-87 0.9152272934307213 1101 88-89 0.6709503463496276 1101 90-91 0.06449796646330341 1101 92-93 -0.2831728079309457 1101 94-95 -0.04499490998657407 1101 96-97 1.1005727390347246 1101 98-99 0.09837576307132778 1101 100-101 0.8611904385838081 1102 1 0.463595231776317 1102 2 -0.09410588781668139 1102 3 0.3722516869388741 1102 4 0.18166009876468792 1102 5 0.19658698974673428 1102 6 0.38621291465581464 1102 7 0.2684817288533381 1102 8 0.23807126496867426 1102 9 0.5014299434391276 1102 10-11 0.25372938607522855 1102 12-13 0.38177151017605837 1102 14-15 0.4772772606245823 1102 16-17 0.6391784313893751 1102 18-19 0.4857961770267991 1102 20-21 0.22517962372253209 1102 22-23 0.38816871337212433 1102 24-25 0.7042670980495771 1102 26-27 0.16211459213641177 1102 28-29 0.7607216512958459 1102 30-31 0.3730739351801873 1102 32-33 0.650452337145154 1102 34-35 1.1047817025692765 1102 36-37 0.9197341738540388 1102 38-39 1.5096371602755667 1102 40-41 1.2127372185048344 1102 42-43 0.9883262928918839 1102 44-45 0.6499421995341947 1102 46-47 1.8556896414312476 1102 48-49 2.364417279924787 1102 50-51 2.915285827166798 1102 52-53 2.538275526254303 1102 54-55 2.7759502299349634 1102 56-57 2.4178386398236995 1102 58-59 2.2530275030490543 1102 60-61 2.3824232284627094 1102 62-63 2.032234737986265 1102 64-65 2.008450170847084 1102 66-67 1.1808774411983016 1102 68-69 2.732886253686406 1102 70-71 3.6038321571690908 1102 72-73 3.369133222576263 1102 74-75 3.839306503519758 1102 76-77 3.441029866413622 1102 78-79 4.10861156302423 1102 80-81 4.404836566490584 1102 82-83 4.135649226086112 1102 84-85 3.5891052731156954 1102 86-87 4.565714882083206 1102 88-89 4.8393900626616855 1102 90-91 4.3419802359668545 1102 92-93 4.1769335750477765 1102 94-95 3.4751735297297373 1102 96-97 3.4104131645666413 1102 98-99 4.136496330447219 1102 100-101 3.561766679718559 1103 1 0.2978150899323424 1103 2 0.19667425402729322 1103 3 0.16347509119419357 1103 4 0.1262522973462481 1103 5 0.4177749330091416 1103 6 0.2319575955068771 1103 7 0.23789662247035892 1103 8 0.4206953784438525 1103 9 -0.14617643953959458 1103 10-11 0.2512914428128141 1103 12-13 0.2530037796796094 1103 14-15 0.524706338638758 1103 16-17 0.5101890696872502 1103 18-19 0.15623057418991948 1103 20-21 0.2415803329423909 1103 22-23 -0.1982674568406395 1103 24-25 0.31242312641837344 1103 26-27 0.31681317369670126 1103 28-29 0.5738865449128667 1103 30-31 0.5765313819887012 1103 32-33 0.3348495002657188 1103 34-35 0.11076574512247106 1103 36-37 0.1730586419391429 1103 38-39 0.49478786949543263 1103 40-41 0.6562212610580289 1103 42-43 0.4067660092039347 1103 44-45 1.257876596697308 1103 46-47 1.303384676892236 1103 48-49 1.7414119607758565 1103 50-51 1.2690092314221175 1103 52-53 0.545589356041539 1103 54-55 1.761765832771843 1103 56-57 1.3566684270577412 1103 58-59 0.07195480801360077 1103 60-61 0.1386289022215763 1103 62-63 -0.060407105985365916 1103 64-65 -0.27811897808908626 1103 66-67 -0.8481562467449635 1103 68-69 -1.631032186029909 1103 70-71 -0.7951040130436766 1103 72-73 -0.5386681958634547 1103 74-75 -1.3000995248490312 1103 76-77 -1.2181013392601336 1103 78-79 -2.2686047490325087 1103 80-81 -1.1162183980484208 1103 82-83 -1.8415227597294894 1103 84-85 -1.8497245141183463 1103 86-87 -0.808176961888428 1103 88-89 -1.9779858238631398 1103 90-91 -1.4993318207707382 1103 92-93 -1.5384919568671194 1103 94-95 -1.4705268248801922 1103 96-97 -2.122831516284421 1103 98-99 -2.476092322035054 1103 100-101 -1.7771074337566155 1104 1 0.19403740864706265 1104 2 0.07596213939420693 1104 3 0.04189454408172821 1104 4 0.030724724615026844 1104 5 0.0456516155970732 1104 6 0.16682515955377397 1104 7 0.3998592798737448 1104 8 0.24657466632921654 1104 9 0.28586871894933097 1104 10-11 0.4805576173677437 1104 12-13 0.5869160679991907 1104 14-15 0.9521922266109684 1104 16-17 0.9669845538383512 1104 18-19 0.5876244083193214 1104 20-21 0.5359789434504236 1104 22-23 0.5527095296986531 1104 24-25 0.284199070838703 1104 26-27 0.45250574859900183 1104 28-29 0.0011553247652287268 1104 30-31 0.37179842497610593 1104 32-33 0.5246019970090927 1104 34-35 0.4970010903243818 1104 36-37 0.8808311126295507 1104 38-39 0.47285994939121423 1104 40-41 0.19551783074972917 1104 42-43 -0.198748537040089 1104 44-45 -0.17807650794880203 1104 46-47 0.5487168523156001 1104 48-49 -0.405353128238481 1104 50-51 -0.07706111160871387 1104 52-53 0.1333010364583842 1104 54-55 0.23853526394857738 1104 56-57 -0.2071613601763005 1104 58-59 -0.8283925649781523 1104 60-61 -0.6694475198366057 1104 62-63 -1.154414921877681 1104 64-65 -1.5227998291529161 1104 66-67 -1.5055596336316341 1104 68-69 -1.6731511612795842 1104 70-71 -1.0951474346676413 1104 72-73 -1.137669498512178 1104 74-75 -0.802487714167313 1104 76-77 -1.1308238750829744 1104 78-79 -2.1009972805131873 1104 80-81 -2.4219066307883246 1104 82-83 -2.52910417527443 1104 84-85 -1.8369151350475725 1104 86-87 -0.5960623288011497 1104 88-89 -1.0602698012838943 1104 90-91 -1.2388020769583186 1104 92-93 -1.6776582616869185 1104 94-95 -2.974868987277066 1104 96-97 -2.4250460191068264 1104 98-99 -2.6738778192126453 1104 100-101 -2.113190803274634 1105 1 0.07007671325779796 1105 2 0.05404226033147097 1105 3 0.48104798323517173 1105 4 0.193234172838757 1105 5 -0.4029500472903038 1105 6 0.19176847021137178 1105 7 0.11338913626074287 1105 8 0.1362194131168195 1105 9 0.21670772121690618 1105 10-11 -0.0668724657766333 1105 12-13 0.035706695361241714 1105 14-15 -0.0018894060420890924 1105 16-17 0.20167843138937513 1105 18-19 -0.024620489639865184 1105 20-21 0.2599018459447535 1105 22-23 0.36733538003879573 1105 24-25 0.21352635730884373 1105 26-27 0.030170147691968907 1105 28-29 -0.21728760796341362 1105 30-31 -0.1431297685235151 1105 32-33 0.131933818626635 1105 34-35 0.07353170256927655 1105 36-37 -0.8661454557755945 1105 38-39 -0.7276313582429523 1105 40-41 -0.8439757444581275 1105 42-43 -0.6783403737747875 1105 44-45 -0.5618633560213624 1105 46-47 -0.7230140622724619 1105 48-49 -1.5950734608159536 1105 50-51 -2.330084543203572 1105 52-53 -2.8610300293012543 1105 54-55 -3.3536793996946628 1105 56-57 -2.346050249065186 1105 58-59 -1.6254447191731636 1105 60-61 -1.5296138085743252 1105 62-63 -1.7061911879396625 1105 64-65 -1.0320590884121756 1105 66-67 -1.4360207069498472 1105 68-69 -0.4129470796469299 1105 70-71 -0.9332048798679473 1105 72-73 -1.6679038144607752 1105 74-75 -1.3956472001839444 1105 76-77 -0.8205442076604506 1105 78-79 -1.680740288827625 1105 80-81 -1.7259504705464508 1105 82-83 -0.7983785516916626 1105 84-85 -1.4618206528102284 1105 86-87 -1.7965536364353127 1105 88-89 -1.642091418819799 1105 90-91 -0.9311679121812979 1105 92-93 -1.1610293879151854 1105 94-95 -1.2620949887887782 1105 96-97 -0.5085683169148396 1105 98-99 -1.7199851510342654 1105 100-101 -0.4278166536147694 1106 1 0.41212464354101996 1106 2 0.2588352886539127 1106 3 0.3023987457624031 1106 4 0.11916009876468792 1106 5 0.3497732642565339 1106 6 0.3224874244597373 1106 7 0.3530405523827511 1106 8 0.10571832379220325 1106 9 0.66442013951756 1106 10-11 0.4271362488203252 1106 12-13 0.5318940591956647 1106 14-15 0.3951694174873239 1106 16-17 0.34873725491878815 1106 18-19 0.3087128436934634 1106 20-21 0.4347384472519451 1106 22-23 0.33302165454859534 1106 24-25 0.2876004313829128 1106 26-27 0.4403008666462185 1106 28-29 0.4047167493350585 1106 30-31 0.01645628812136124 1106 32-33 0.3073150822431927 1106 34-35 0.07843366335358581 1106 36-37 0.3076018209128648 1106 38-39 0.07029892498145074 1106 40-41 0.06506564987738983 1106 42-43 -0.4136344914218455 1106 44-45 -0.8347391730148317 1106 46-47 -0.8428397703334625 1106 48-49 -0.9229601710556068 1106 50-51 -0.28814554538222126 1106 52-53 0.05727062429351548 1106 54-55 -0.5965987896728748 1106 56-57 -0.9816711640978681 1106 58-59 -0.305183281264668 1106 60-61 -0.03179245781179674 1106 62-63 0.3569896399470487 1106 64-65 -0.7875057115058581 1106 66-67 -2.0623823627232696 1106 68-69 -1.643339236509675 1106 70-71 -0.9010698036152256 1106 72-73 -0.35635697350216944 1106 74-75 -0.794884672950829 1106 76-77 0.11627496445283825 1106 78-79 -1.0641825546228283 1106 80-81 -1.4193055903721579 1106 82-83 -1.652953715090355 1106 84-85 -1.129031981786266 1106 86-87 -0.32215276497561973 1106 88-89 -1.0674726824363567 1106 90-91 -0.9594903522684426 1106 92-93 -1.7764977975012428 1106 94-95 -1.3906352937996722 1106 96-97 -1.3898319334725748 1106 98-99 -1.4639938656312133 1106 100-101 -1.383699006555947 1107 1 0.2552618984429813 1107 2 0.3426757213787255 1107 3 0.3226827214216357 1107 4 0.06168883439686823 1107 5 0.33523641503408896 1107 6 -0.12240777499935973 1107 7 -0.15608723666390034 1107 8 -0.1383655166405262 1107 9 -0.23348384966431723 1107 10-11 0.1010713400982084 1107 12-13 -0.1695934323526771 1107 14-15 0.06707611119929169 1107 16-17 0.34823015552731107 1107 18-19 -0.08496531722607159 1107 20-21 0.10089801452713232 1107 22-23 -0.4458830107658116 1107 24-25 -0.21527313183548102 1107 26-27 -0.4276842584382976 1107 28-29 -0.03561455560070925 1107 30-31 -0.2842536510267095 1107 32-33 0.1561994635819275 1107 34-35 0.7114627370520381 1107 36-37 0.3841738290264516 1107 38-39 0.4575538269422381 1107 40-41 0.61036652884966 1107 42-43 -0.6007541668782324 1107 44-45 -0.26780205333937346 1107 46-47 0.07264366441975056 1107 48-49 -0.49226375455797466 1107 50-51 -0.6407486555918211 1107 52-53 -0.12443999098707792 1107 54-55 -0.32462448270871036 1107 56-57 -0.0778510153487133 1107 58-59 -0.22793514062909992 1107 60-61 -0.6420020588936097 1107 62-63 -0.9498054918987897 1107 64-65 -1.0400412084632613 1107 66-67 -1.4028150875373306 1107 68-69 -1.6026022520607235 1107 70-71 -1.9478919807619413 1107 72-73 -2.214200110757073 1107 74-75 -2.7206791286641483 1107 76-77 -2.3211827772645357 1107 78-79 -2.2225665978953124 1107 80-81 -1.4504076863829773 1107 82-83 -0.7131295095460679 1107 84-85 -0.9496878303325822 1107 86-87 -1.8355063822846134 1107 88-89 -1.588770856878547 1107 90-91 -1.26290482150441 1107 92-93 -1.1980664249522235 1107 94-95 -0.9389787691208333 1107 96-97 -1.3288109733643907 1107 98-99 -0.7959749339206006 1107 100-101 -1.3387361938446567 1108 1 -0.2685476253665442 1108 2 -0.14172493543572529 1108 3 -0.2755657733785881 1108 4 0.07154105114563691 1108 5 0.022975878635620006 1108 6 0.2870065654494667 1108 7 -0.04005001717840884 1108 8 -0.04367476677735738 1108 9 0.14527914978833678 1108 10-11 0.019106370202209177 1108 12-13 0.052902462557014474 1108 14-15 0.4623963082436262 1108 16-17 -0.012607282896340166 1108 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0.4880037871238656 2316 54-55 0.05674733138424415 2316 56-57 -0.17455266452412843 2316 58-59 -0.7256862650668836 2316 60-61 -0.5179390903778689 2316 62-63 -0.499468160564458 2316 64-65 -0.3054085248050882 2316 66-67 0.12698251366206748 2316 68-69 0.9348790073095934 2316 70-71 0.19078867890821982 2316 72-73 -0.46963489336576814 2316 74-75 0.4294333151139611 2316 76-77 0.9745443591672434 2316 78-79 -0.21113481378736765 2316 80-81 0.20963729112826712 2316 82-83 0.6225151681151004 2316 84-85 1.2358444035504768 2316 86-87 0.6929793748368276 2316 88-89 0.35388281628487306 2316 90-91 1.0249512504596048 2316 92-93 0.20410748809125323 2316 94-95 -0.2834315427340286 2316 96-97 -0.032521618042054 2316 98-99 0.672728214505188 2316 100-101 0.39645870870406696 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 0.0 13 2.0 14 23.0 15 53.0 16 188.0 17 542.0 18 929.0 19 1228.0 20 1798.0 21 2680.0 22 2782.0 23 1913.0 24 2107.0 25 2314.0 26 3044.0 27 3543.0 28 4203.0 29 4143.0 30 2369.0 31 1061.0 32 763.0 33 775.0 34 998.0 35 1541.0 36 3129.0 37 10180.0 38 8537.0 39 233.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.178087799446544 19.564113900214505 14.194953414877764 22.062844885461185 2 11.527188774090023 22.70233982283497 42.9584268007139 22.81204460236111 3 21.25499443243597 33.48726010349119 36.33981790790594 8.917927556166896 4 8.167976424361493 25.559921414538312 29.12901113294041 37.143091028159795 5 6.77200163733115 42.4887433483422 34.460908718788374 16.278346295538274 6 20.541912246234446 39.39259986902423 15.915193189259988 24.150294695481335 7 17.003929273084477 38.51342501637197 15.214472822527833 29.268172888015716 8 27.30517354289456 27.652259332023576 20.800589390962674 24.24197773411919 9 22.85527177472168 15.322527832351016 16.168958742632615 45.653241650294696 10-11 21.73870333988212 27.748035363457763 22.65635232481991 27.856908971840213 12-13 31.796823837590043 23.843320235756384 21.405533726260643 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16.944171578258022 24.769155206286836 35.550916830386385 22.73575638506876 40-41 14.43213727271983 26.83072590482272 36.699658619249945 22.037478203207506 42-43 19.21087098886706 25.77521283562541 35.486247544204325 19.527668631303207 44-45 15.400420763103822 27.139220196628983 36.47705040152588 20.983308638741313 46-47 22.18847721802911 24.401185350120336 30.93207157942992 22.478265852420638 48-49 19.561231172233136 23.13523248199083 33.69106090373281 23.61247544204322 50-51 13.17861820563196 26.177144728225276 38.48968565815324 22.154551407989523 52-53 13.770464963981663 30.25458415193189 37.07269155206287 18.902259332023576 54-55 12.315198349678285 28.619492788028616 31.204669362628728 27.86063949966437 56-57 20.048625550516544 29.71397698063164 23.621866762717136 26.615530706134678 58-59 16.40635232481991 32.35920104780616 30.985592665356908 20.248853962017026 60-61 18.24246889325475 38.923542894564505 23.959561231172234 18.87442698100851 62-63 10.958668620404556 44.18258171727011 23.803403760672566 21.055345901652764 64-65 10.807492059333965 46.099250139166315 22.312452929041555 20.78080487245817 66-67 13.073554091113749 40.400311080185006 22.397773320780974 24.128361507920264 68-69 12.968162877702552 38.48861673475068 24.719818588164024 23.82340179938274 70-71 15.861126119488514 37.04504150498551 24.531329305630596 22.562503069895378 72-73 15.191407145430134 32.316534040671975 29.40613026819923 23.08592854569866 74-75 13.636028719493726 35.21085249740067 27.308079608995715 23.845039174109882 76-77 14.491555257190106 30.598377364978262 28.49435516222257 26.415712215609062 78-79 15.485428945645054 30.764571054354946 29.69056974459725 24.05943025540275 80-81 16.964124899710185 29.74964386881273 28.85563178491314 24.430599446563946 82-83 15.167467640388724 28.930843355738766 30.443824042311064 25.457864961561448 84-85 14.237876517737988 30.64459345499799 28.501133953937725 26.616396073326293 86-87 14.737428155037744 30.408226759894546 25.535050516628733 29.31929456843898 88-89 15.048913265932626 29.28574352257378 23.669927551062177 31.99541566043142 90-91 16.97269862060497 25.549506774180347 24.405877778232572 33.07191682698211 92-93 17.91009341050684 26.820522476647373 24.14182678531957 31.12755732752622 94-95 16.83477150295548 25.670099716732437 23.829679236323745 33.66544954398834 96-97 14.200335666625733 25.636743215031316 25.996152114290393 34.16676900405256 98-99 17.522287622899153 26.5020097746269 20.99580034874297 34.97990225373098 100-101 16.015362693876213 24.962739735001723 21.740340992842754 37.281556578279314 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 45.0 1 25.5 2 21.5 3 45.0 4 32.0 5 13.5 6 24.5 7 48.0 8 63.0 9 57.0 10 41.0 11 31.0 12 35.0 13 37.0 14 36.0 15 39.5 16 45.5 17 53.0 18 71.5 19 88.5 20 104.0 21 140.5 22 200.5 23 244.0 24 282.5 25 387.5 26 491.5 27 594.0 28 722.0 29 832.0 30 951.5 31 1094.0 32 1270.5 33 1392.5 34 1430.5 35 1521.5 36 1654.5 37 1748.0 38 1828.0 39 1881.5 40 1871.0 41 1843.0 42 1840.5 43 1818.5 44 1721.5 45 1673.0 46 1742.0 47 3117.0 48 6164.5 49 6051.5 50 4180.5 51 3351.5 52 2114.5 53 1182.0 54 557.5 55 482.5 56 473.0 57 347.5 58 237.5 59 200.0 60 152.5 61 69.0 62 43.5 63 30.0 64 27.0 65 33.0 66 37.5 67 37.0 68 29.0 69 17.0 70 11.0 71 7.5 72 3.0 73 1.5 74 3.5 75 3.0 76 0.5 77 0.0 78 0.5 79 1.0 80 0.5 81 1.0 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014734774066797643 2 0.0114603798297315 3 0.019646365422396856 4 0.0 5 0.008185985592665358 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0016371971185330713 28-29 0.0032743942370661427 30-31 0.0016371971185330713 32-33 0.0032743942370661427 34-35 0.006548788474132285 36-37 8.185985592665357E-4 38-39 0.0 40-41 0.0073673870333988214 42-43 0.0 44-45 8.185985592665357E-4 46-47 0.0016371971185330713 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0016371971185330713 56-57 0.0016371971185330713 58-59 0.0 60-61 0.0 62-63 8.185985592665357E-4 64-65 0.0032743942370661427 66-67 0.004092992796332679 68-69 0.00573018991486575 70-71 0.004911591355599214 72-73 0.009823182711198428 74-75 0.010641781270464964 76-77 0.010641781270464964 78-79 0.0 80-81 0.0114603798297315 82-83 0.013916175507531107 84-85 0.01719056974459725 86-87 0.018009168303863787 88-89 0.004092992796332679 90-91 0.004092992796332679 92-93 0.009004584151931894 94-95 0.0114603798297315 96-97 0.012278978388998035 98-99 0.00573018991486575 100-101 0.03765553372626064 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 61080.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.410281597904387 #Duplication Level Percentage of deduplicated Percentage of total 1 80.8168403384852 18.919449901768175 2 8.692915588502693 4.070072036673215 3 3.2100146863417023 2.2544204322200394 4 1.6994195398279601 1.5913555992141455 5 0.9651024547171131 1.1296660117878194 6 0.6573886285754249 0.9233791748526522 7 0.3846422826771103 0.6303208906352326 8 0.3496748024337366 0.6548788474132285 9 0.3217008182390377 0.6777996070726915 >10 2.133016294845793 9.74950884086444 >50 0.33568781033638717 5.3388998035363455 >100 0.3217008182390377 16.756712508185988 >500 0.06294146443807258 10.11787819253438 >1k 0.041960976292048396 17.066142763588736 >5k 0.006993496048674733 10.119515389652914 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 6181 10.119515389652914 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 2270 3.7164374590700717 RNA PCR Primer, Index 26 (100% over 22bp) GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2113 3.45939751146038 No Hit ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT 1957 3.2039947609692208 RNA PCR Primer, Index 26 (100% over 24bp) TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG 1765 2.889652914210871 RNA PCR Primer, Index 26 (100% over 29bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1275 2.0874263261296657 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1044 1.7092337917485263 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 988 1.6175507531106743 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 775 1.2688277668631303 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 725 1.1869679109364768 No Hit CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT 696 1.1394891944990178 RNA PCR Primer, Index 26 (100% over 27bp) GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 675 1.1051080550098231 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 606 0.9921414538310411 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 604 0.9888670595939751 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 570 0.9332023575638507 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 541 0.8857236411263916 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 496 0.8120497707924035 No Hit CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG 485 0.7940406024885396 RNA PCR Primer, Index 26 (96% over 27bp) ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 459 0.7514734774066798 No Hit CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA 441 0.7220039292730845 RNA PCR Primer, Index 26 (100% over 29bp) GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 400 0.6548788474132285 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 372 0.6090373280943026 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 352 0.5762933857236412 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 345 0.5648330058939097 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 343 0.5615586116568435 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 343 0.5615586116568435 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 292 0.4780615586116569 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 283 0.4633267845448592 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 249 0.40766208251473474 No Hit ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC 239 0.39129011132940406 RNA PCR Primer, Index 26 (96% over 28bp) GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 237 0.3880157170923379 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 233 0.38146692861820564 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 232 0.3798297314996726 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 231 0.3781925343811395 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 205 0.33562540929927964 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 205 0.33562540929927964 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC 204 0.3339882121807466 No Hit TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC 202 0.33071381794368043 RNA PCR Primer, Index 26 (95% over 22bp) TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT 188 0.30779305828421744 RNA PCR Primer, Index 26 (100% over 21bp) GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 183 0.29960707269155207 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 178 0.2914210870988867 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 172 0.2815979043876883 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 156 0.2554027504911591 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT 156 0.2554027504911591 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 152 0.24885396201702686 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 150 0.2455795677799607 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA 148 0.24230517354289455 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 147 0.24066797642436152 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 146 0.23903077930582844 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 137 0.2242960052390308 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 136 0.2226588081204977 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 131 0.21447282252783237 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 129 0.2111984282907662 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 129 0.2111984282907662 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 127 0.20792403405370005 No Hit GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 127 0.20792403405370005 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA 127 0.20792403405370005 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 121 0.19810085134250163 No Hit TAATAAGGCTGGGCAAGGTGGCTCATGCCTGTAATCCCAGCTCTTAGGGA 120 0.19646365422396855 No Hit CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC 115 0.1882776686313032 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA 109 0.1784544859201048 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 103 0.16863130320890635 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 94 0.15389652914210872 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 92 0.15062213490504256 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT 92 0.15062213490504256 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 91 0.1489849377865095 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 87 0.14243614931237722 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 84 0.137524557956778 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT 84 0.137524557956778 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 82 0.13425016371971185 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 81 0.13261296660117877 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 80 0.13097576948264572 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 80 0.13097576948264572 No Hit GTACATGGGAAGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA 80 0.13097576948264572 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 75 0.12278978388998035 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 73 0.11951538965291422 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 73 0.11951538965291422 No Hit GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCCTGAG 72 0.11787819253438114 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 71 0.11624099541584806 No Hit GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 70 0.11460379829731501 No Hit ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTT 69 0.11296660117878193 RNA PCR Primer, Index 26 (96% over 30bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 67 0.10969220694171578 No Hit TATCAACGCAGAGTACCCATGTACTCTGCGTTGATACCACTGCTTCCCAT 67 0.10969220694171578 No Hit GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 66 0.10805500982318272 No Hit GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCCTGA 64 0.10478061558611657 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 64 0.10478061558611657 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 63 0.10314341846758349 No Hit GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA 63 0.10314341846758349 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 62 0.10150622134905044 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0016371971185330713 0.0 5 0.0 0.0 0.0 0.0016371971185330713 0.0 6 0.0 0.0 0.0 0.0016371971185330713 0.0 7 0.0 0.0 0.0 0.0016371971185330713 0.0 8 0.0 0.0 0.0 0.0016371971185330713 0.0 9 0.0 0.0 0.0 0.0016371971185330713 0.0 10-11 0.0 0.0 0.0 0.0016371971185330713 0.0 12-13 0.0 0.0 0.0 0.0016371971185330713 0.0 14-15 0.0 0.0 0.0 0.008185985592665358 0.0 16-17 0.0 0.0 0.0 0.013916175507531107 0.0 18-19 0.0 0.0 0.0 0.020464963981663392 0.0 20-21 0.0 0.0 0.0 0.03028814669286182 0.0 22-23 0.0 0.0 0.0 0.04911591355599214 0.0 24-25 0.0 0.0 0.0 0.09659462999345121 0.0 26-27 0.0 0.0 0.0 0.14816633922724298 0.0 28-29 0.0 0.0 0.0 0.28487229862475444 0.0 30-31 0.0 0.0 0.0 0.5566470203012444 0.0 32-33 0.0 0.0 0.0 0.8218729535036018 0.0 34-35 0.0 0.0 0.0 1.2982973149967256 0.0 36-37 0.0 0.0 0.0 1.8361165684348395 0.0 38-39 0.0 0.0 0.0 2.4607072691552063 0.0 40-41 0.0 0.0 0.0 3.4954158480681077 0.0 42-43 0.0 0.0 0.0 4.1535690897184026 0.0 44-45 0.0 0.0 0.0 4.553045186640471 0.0 46-47 0.0 0.0 0.0 4.97871643745907 0.0 48-49 0.0 0.0 0.0 5.3740995415848065 0.0 50-51 0.0 0.0 0.0 5.777668631303209 0.0 52-53 0.0 0.0 0.0 6.236083824492469 0.0 54-55 0.0 0.0 0.0 6.614276358873608 0.0 56-57 0.0 0.0 0.0 6.94253438113949 0.0 58-59 0.0 0.0 0.0 7.185658153241651 0.0 60-61 0.0 0.0 0.0 7.405861165684348 0.0 62-63 0.0 0.0 0.0 7.6530779305828425 0.0 64-65 0.0 0.0 0.0 7.8896529142108704 0.0 66-67 0.0 0.0 0.0 8.233464309102816 0.0 68-69 0.0 0.0 0.0 8.7524557956778 0.0 70-71 0.0 0.0 0.0 9.25098231827112 0.0 72-73 0.0 0.0 0.0 9.63408644400786 0.0 74-75 0.0 0.0 0.0 10.033562540929928 0.0 76-77 0.0 0.0 0.0 10.34053700065488 0.0 78-79 0.0 0.0 0.0 10.704813359528487 0.0 80-81 0.0 0.0 0.0 11.129666011787819 0.0 82-83 0.0 0.0 0.0 11.50540275049116 0.0 84-85 0.0 0.0 0.0 11.878683693516699 0.0 86-87 0.0 0.0 0.0 12.252783235101507 0.0 88-89 0.0 0.0 0.0 12.661263916175507 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCTCGG 15 6.594465E-4 94.97543 1 ATAAGGC 35 3.3836113E-6 67.83959 3 TAATAAG 35 3.3836113E-6 67.83959 1 TAAGGCT 35 3.3836113E-6 67.83959 4 CTGGGCA 40 7.4720065E-6 59.359646 9 AAGGCTG 40 7.4720065E-6 59.359646 5 AATAAGG 40 7.4720065E-6 59.359646 2 TATATGG 25 0.005003712 56.985256 2 TCGGAGA 25 0.005003712 56.985256 4 GTATATG 25 0.005003712 56.985256 1 GCTCGGA 25 0.005003712 56.985256 2 CTCGGAG 25 0.005003712 56.985256 3 AAGTACT 35 2.7705694E-4 54.27167 4 GGCTGGG 45 1.5012678E-5 52.76413 7 AAAGTAC 40 5.3547684E-4 47.487717 3 GGTACTC 20 5.376016E-4 47.487717 50-51 GCTGGGC 50 2.799689E-5 47.487713 8 AGTACTC 45 9.565687E-4 42.211304 5 GAAGGCA 70 4.2509273E-6 40.703754 9 CCTGTAA 30 8.220023E-5 39.60553 28-29 >>END_MODULE