FastQCFastQC Report
Fri 10 Feb 2017
SRR2568619_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568619_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1482453
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28750.044.144181
GGTATCA14500.042.2884521
TCAACGC30900.039.8393254
ATCAACG31750.039.0748063
CAACGCA31400.039.052255
TATCAAC34300.036.7241552
AACGCAG33450.036.6551976
CGCAGAG37850.031.8918558
ACGCAGA38650.031.4776577
GCAGAGT46800.025.0820529
TATACGA950.001222408525.015115
GTATATA15150.021.9626871
GTACATG43500.021.7452241
GTATAGC8900.021.3633841
TACATGG42150.021.3139672
AGAGTAC42400.020.95982710-11
ACATGGG40800.020.854173
GTCTAAG5350.020.4349751
GTATAAT12800.020.4245621
CAGAGTA46950.018.47307610-11
Produced by FastQC (version 0.11.5)