FastQCFastQC Report
Fri 10 Feb 2017
SRR2568619_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568619_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1482453
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18680.12600736751856553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17100.052.5177151
GTATCAA30400.038.2941671
ATCAACG34550.032.725113
TCAACGC34550.032.5810134
CAACGCA34950.032.2081265
AACGCAG36650.030.7131296
TATCAAC37700.030.6229082
TATACCG1354.2290048E-728.146255
ACGCAGA41450.027.0418977
CGCAGAG41450.026.8127278
GCAGAGT51000.021.6056589
ACATGGG40350.021.1924513
GTACATG43400.020.8019471
TACATGG43200.019.9056512
CGTAGAC1200.004760422319.7943363
AGAGTAC44850.019.59110510-11
CAGAGTA48500.019.43875510-11
ATAGACG2251.6699141E-618.9980778
GAGTACT26600.018.74810412-13
GTATAGC8550.017.7837771
Produced by FastQC (version 0.11.5)