FastQCFastQC Report
Fri 10 Feb 2017
SRR2568618_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568618_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4388553
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39600.042.6071741
GTATCAA70200.036.0860751
TCAACGC69500.034.8070874
ATCAACG71250.034.086753
CAACGCA74950.032.2119375
AACGCAG76200.031.8677186
GTACATG145850.031.2182541
ACATGGG149300.028.714173
TACATGG157050.028.2353552
TATCAAC87000.028.0251332
ACGCAGA86800.027.8117967
CGCAGAG88700.027.0553288
TAGTCCG1257.3731335E-626.611129
CATGGGG80150.024.370984
CATGGGA69400.022.804444
GCAGAGT108950.021.5464069
TATACTG29050.019.3045925
CTTAATA33800.017.998772
GTATATA27300.017.583621
GTCCGTA1901.8587935E-417.5103151
Produced by FastQC (version 0.11.5)