FastQCFastQC Report
Fri 10 Feb 2017
SRR2568618_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568618_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4388553
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39750.044.945131
GTATCAA68150.036.4642871
TCAACGC70050.034.446024
ATCAACG71900.033.565073
CAACGCA75400.032.0019045
GTACATG142150.031.587621
AACGCAG77350.031.0687776
TACATGG152050.028.7460982
ACATGGG146150.028.6696573
TATCAAC87350.028.2825092
ACGCAGA89600.026.7680857
CGCAGAG91050.026.0288248
CATGGGG79100.025.5208954
CATGGGA63850.022.9869334
TATACTG31100.022.7567025
GCAGAGT113600.020.7365889
GTACCGT7050.018.8626586
ATGGGGG40700.018.0892565
CGTATAC4500.017.9468883
ATAGCCC15150.017.873723
Produced by FastQC (version 0.11.5)