FastQCFastQC Report
Fri 10 Feb 2017
SRR2568617_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568617_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21965
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN360.16389710903710447No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.11837013430457546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.10471204188481677No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACAC156.530825E-495.03
TAACACT156.530825E-495.04
GTACAGT200.002046931871.256
CATAGGT250.004955927457.04
GGTATCA250.004955927457.01
TATCAAC500.001581441338.01
GTACATG550.002524988534.5454561
CAACGCA550.002524988534.5454564
AACGCAG600.003867640631.6666685
ACATGGG600.003867640631.6666683
CGCAGAG650.005721388429.230777
ACGCAGA700.0082161927.1428556
TACATGG700.0082161927.1428552
CAGAGTA650.00745053421.9230779
AGAGTAC650.00745053418.26923210-11
Produced by FastQC (version 0.11.5)