FastQCFastQC Report
Fri 10 Feb 2017
SRR2568617_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568617_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21965
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.15934441156385157No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.10926473935806967No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGT250.004956078757.02
GTATCAA405.2728044E-447.5000041
CAACGCA554.8053516E-543.181824
ATCAACG554.8053516E-543.181822
TCAACGC554.8053516E-543.181823
AACGCAG608.027848E-539.5833365
TATCAAC651.286219E-436.5384641
ACGCAGA651.286219E-436.5384646
CAGAGTA651.286219E-436.5384649
GTACTTT300.003910054531.66666814-15
CGCAGAG752.9818827E-431.6666687
GCAGAGT752.9818827E-431.6666688
GAGTACT350.00830548227.14285512-13
ACTTTTT500.001628531123.7516-17
AGAGTAC701.7294602E-523.74999810-11
Produced by FastQC (version 0.11.5)