FastQCFastQC Report
Fri 10 Feb 2017
SRR2568616_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568616_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3216482
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC36880.11465943226170705No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28550.045.10991
GTATCAA49500.042.2431371
TCAACGC57500.036.034114
ATCAACG59100.035.059663
CAACGCA60200.034.416895
AACGCAG61750.033.5514146
GTACATG129650.032.329931
TATCAAC66900.031.0430242
TACATGG139350.029.3291972
ACATGGG135450.029.2965283
ACGCAGA71850.028.5052817
CGCAGAG73900.027.5207758
CATGGGG70850.027.165044
ACCGCGC1050.002192148522.6277838
TATTAAC22300.021.737222
GCAGAGT91800.021.6886589
CTATTAA21900.020.3982281
CATGGGA56650.020.384574
GTATTAT12850.019.6011451
TAATACT28100.019.4485264
Produced by FastQC (version 0.11.5)