FastQCFastQC Report
Fri 10 Feb 2017
SRR2568616_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568616_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3216482
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT46610.14490987358237975No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC42170.13110597230141505No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28000.047.5150151
GTATCAA45400.043.0146941
TCAACGC54150.034.7343184
CAACGCA56250.033.437575
ATCAACG56650.033.3733063
GTACATG127700.032.780521
AACGCAG58100.032.6978266
ACATGGG130600.030.6256283
TACATGG138700.029.8664152
TATCAAC63750.029.8073162
ACGCAGA68400.027.3574247
TACCGTA2650.026.8831677
CGCAGAG71350.025.8934948
CATGGGG68000.025.2849274
CATGGGA53600.024.191344
TAATACT29600.021.98324
TTAATAC28350.021.1122043
GTACCGT3850.020.9711996
CATGGGC34350.020.1877614
GCAGAGT92350.019.9025699
Produced by FastQC (version 0.11.5)