FastQCFastQC Report
Fri 10 Feb 2017
SRR2568615_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568615_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3088333
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46100.039.2782061
TCAACGC49200.034.675984
CAACGCA49200.034.674855
GGTATCA25150.034.2033121
ATCAACG51850.032.81423
AACGCAG54500.031.4756586
GTACATG117850.031.172921
TATCAAC58600.029.4399192
ACATGGG120800.028.4838833
TACATGG126500.028.2148022
CATGGGG77650.026.4999984
ACGCAGA67550.025.1151167
CGCAGAG67100.025.0706758
TAATACT20150.022.4051554
GTATAGG11600.021.3045791
GTATAGA7950.020.923221
CGTATAC1150.00371307120.6633013
ATGGGGA38300.019.85215
TACCGTA4350.019.6641677
GCAGAGT85300.019.3311949
Produced by FastQC (version 0.11.5)