FastQCFastQC Report
Fri 10 Feb 2017
SRR2568615_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568615_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3088333
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45950.035.679421
GGTATCA25600.035.640641
CAACGCA47500.033.502775
TCAACGC47850.033.2577134
GTACATG113400.031.8481921
ATCAACG52400.030.6468093
AACGCAG52150.030.5105236
ACATGGG116300.029.5773123
TACATGG121600.029.0719622
TATCAAC57200.028.5757352
CATGGGG76150.025.5142564
ACGCAGA62450.025.25027
CGCAGAG66750.023.410138
CATGGGA44500.021.3500794
GAGTACA80000.020.552771
GTACGCT3003.7125574E-919.0046483
GTATTAC6600.018.7203371
GTATATA8900.018.6879771
GCAGAGT84100.018.5805749
ATGGGGA37900.018.5503445
Produced by FastQC (version 0.11.5)