FastQCFastQC Report
Fri 10 Feb 2017
SRR2568614_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568614_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2461751
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21450.049.406191
GTATCAA35300.040.2531851
TCAACGC38950.034.1607864
CAACGCA41000.032.450765
ATCAACG41100.032.3757633
GTACATG85350.030.345671
AACGCAG44250.030.2815236
TACATGG84100.029.7795682
TATCAAC45600.029.7018682
ACATGGG83350.028.7361583
ACGCAGA52000.025.403427
CGCAGAG52450.025.0037698
CATGGGG56000.023.4206164
TAAGACG2005.3725125E-721.3827345
CATGGGA31750.020.205394
GTATTAG6050.019.63761
GCAGAGT67550.018.8517349
GTATATG14000.018.6697461
AGAGTAC55700.017.78659210-11
ATGGGGG29450.017.7483545
Produced by FastQC (version 0.11.5)