FastQCFastQC Report
Fri 10 Feb 2017
SRR2568614_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568614_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2461751
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33600.044.266421
GGTATCA18850.043.86391
TCAACGC41150.035.3226474
CAACGCA42150.034.5973245
ATCAACG43250.033.5018163
AACGCAG45650.032.0442356
TATCAAC47500.030.7068082
GTACATG89000.028.3513551
TACATGG86500.028.3415452
ACATGGG88150.027.000393
ACGCAGA55250.026.5623287
ATAACGC1109.471887E-525.9127313
CGCAGAG56650.025.8220528
CATGGGG59300.023.630294
TAACGCA1656.325566E-520.151894
GCAGAGT73650.019.6038239
AGAGTAC59750.019.03739510-11
GGGCGTA2001.2870789E-518.9976529
GTATAGA10850.018.8325021
GACGTAT2302.0518473E-618.5944791
Produced by FastQC (version 0.11.5)