FastQCFastQC Report
Fri 10 Feb 2017
SRR2568613_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568613_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809606
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26750.045.1211361
GGTATCA15800.042.1057131
TCAACGC32850.036.01924
CAACGCA33550.035.4083375
ATCAACG33950.034.8521543
AACGCAG35950.033.3079346
TATCAAC38150.031.1397782
GTACATG65400.030.8076231
TACATGG66950.029.5265582
ACGCAGA40600.029.2590337
ACATGGG68100.028.3999353
CGCAGAG41900.027.6708058
TAGATCG1000.001650103523.7596514
CATGGGG46250.023.11754
GTATAAT13750.022.463671
GCAGAGT52050.021.6358349
AGAGTAC43450.020.45021210-11
GTATATA10250.020.398531
GTATAGG5400.020.2397021
CATGGGA25250.019.7605024
Produced by FastQC (version 0.11.5)