FastQCFastQC Report
Fri 10 Feb 2017
SRR2568613_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568613_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809606
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16750.047.9506761
GTATCAA28900.045.2228281
TCAACGC34000.037.8624844
CAACGCA34300.037.669825
ATCAACG34650.037.156333
AACGCAG37050.035.1244136
TATCAAC38300.033.8708952
GTACATG67450.030.9318161
ACGCAGA42650.030.5125337
TACATGG66600.030.3233722
CGCAGAG43750.029.6368038
ACATGGG66100.029.1085343
TATTACG908.929207E-426.3991722
CATGGGG44550.024.7376824
GCAGAGT56700.022.2815469
GTATAAT12250.020.949831
AGAGTAC46050.020.36967810-11
TAGTACG1200.00476294319.792814
GTATAAG7950.019.727391
CATGGGA24650.019.2708914
Produced by FastQC (version 0.11.5)